Ndufaf3 (NM_001033971) Rat Tagged ORF Clone
CAT#: RR200350
- TrueORF®
Ndufaf3 (Myc-DDK-tagged ORF) - Rat nuclear protein E3-3 (RGD708545), transcript variant 2, (10 ug)
"NM_001033971" in other vectors (3)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ndufaf3 |
Synonyms | RGD708545 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200350 representing NM_001033971
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTGCTCTAGGATTTCGCTGCCTTTACCGAACGCGACCTGCGACGCTCGGTCGGAGGAGCCCGA GTCGGGGGCACCGGCTTTCTCCGGCTGACGATGAGTTATACCAGCGGACGCGTATCTCTTTGCTGCAAAA CGAGTTCCCTCAGGCCGTATACATCGACAGCTACAATAGCAGAGGTTTCACTATCAACGGAAACCGCGTG TTTGGACCTTGTGCGCTGCTCCCACAGACCGTGGTCCAGTGGAACGTGGGATCTCATCAGGATATCACTG AAGAGAGCTTCTCGATTTTCTGGATGCTGGAACCTAGGATAGAGATTGTTGTGGTGGGCACCGGAAACAA GACTGAGCGGCTGCACTCTCAAGTCCTGCAGGCCATGAGGCAGCGGGGAATTGCGGTGGAGATACAGGAC ACGCCTAACGCCTGTGCCACCTTCAACTTCCTGTGCCACGAAGGTCGAGTGACAGGAGCTGCCCTCATCC CCCCACCTGGAGAGACTGCACTTGCCTCTTCTGGTCAAACCACAGAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR200350 representing NM_001033971
Red=Cloning site Green=Tags(s) MATALGFRCLYRTRPATLGRRSPSRGHRLSPADDELYQRTRISLLQNEFPQAVYIDSYNSRGFTINGNRV FGPCALLPQTVVQWNVGSHQDITEESFSIFWMLEPRIEIVVVGTGNKTERLHSQVLQAMRQRGIAVEIQD TPNACATFNFLCHEGRVTGAALIPPPGETALASSGQTTE SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001033971 |
ORF Size | 537 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033971.1, NM_001033971.2, NP_001029143.1 |
RefSeq Size | 1447 bp |
RefSeq ORF | 540 bp |
Locus ID | 56769 |
Cytogenetics | 8q32 |
MW | 19.9 kDa |
Gene Summary | This gene encodes a protein that is required for assembly of mitochondrial NADH: ubiquinone oxidoreductase complex I, a multiprotein enzyme complex that is part of the oxidative phosphorylation pathway. In humans, mutations in this gene are associated with fatal neonatal mitochondrial disease and lactic acidosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200350 | Ndufaf3 (untagged ORF) - Rat nuclear protein E3-3 (RGD708545), transcript variant 2, (10 ug) |
USD 420.00 |
|
RR200350L3 | Lenti ORF clone of Ndufaf3 (Myc-DDK-tagged ORF) - Rat nuclear protein E3-3 (RGD708545), transcript variant 2, (10 ug) |
USD 640.00 |
|
RR200350L4 | Lenti ORF clone of Ndufaf3 (mGFP-tagged ORF) - Rat nuclear protein E3-3 (RGD708545), transcript variant 2, (10 ug) |
USD 700.00 |
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