Ndufaf3 (NM_001033971) Rat Untagged Clone

CAT#: RN200350

Ndufaf3 (untagged ORF) - Rat nuclear protein E3-3 (RGD708545), transcript variant 2, (10 ug)


  "NM_001033971" in other vectors (3)

Reconstitution Protocol

USD 420.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Symbol Ndufaf3
Synonyms RGD708545
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200350 representing NM_001033971
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACTGCTCTAGGATTTCGCTGCCTTTACCGAACGCGACCTGCGACGCTCGGTCGGAGGAGCCCGA
GTCGGGGGCACCGGCTTTCTCCGGCTGACGATGAGTTATACCAGCGGACGCGTATCTCTTTGCTGCAAAA
CGAGTTCCCTCAGGCCGTATACATCGACAGCTACAATAGCAGAGGTTTCACTATCAACGGAAACCGCGTG
TTTGGACCTTGTGCGCTGCTCCCACAGACCGTGGTCCAGTGGAACGTGGGATCTCATCAGGATATCACTG
AAGAGAGCTTCTCGATTTTCTGGATGCTGGAACCTAGGATAGAGATTGTTGTGGTGGGCACCGGAAACAA
GACTGAGCGGCTGCACTCTCAAGTCCTGCAGGCCATGAGGCAGCGGGGAATTGCGGTGGAGATACAGGAC
ACGCCTAACGCCTGTGCCACCTTCAACTTCCTGTGCCACGAAGGTCGAGTGACAGGAGCTGCCCTCATCC
CCCCACCTGGAGAGACTGCACTTGCCTCTTCTGGTCAAACCACAGAGTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001033971
Insert Size 540 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001033971.2, NP_001029143.1
RefSeq Size 1447 bp
RefSeq ORF 540 bp
Locus ID 56769
Cytogenetics 8q32
Gene Summary This gene encodes a protein that is required for assembly of mitochondrial NADH: ubiquinone oxidoreductase complex I, a multiprotein enzyme complex that is part of the oxidative phosphorylation pathway. In humans, mutations in this gene are associated with fatal neonatal mitochondrial disease and lactic acidosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. It encodes isoform b, which is shorter than isoform a.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.