Gria4 (NM_017263) Rat Tagged ORF Clone

CAT#: RR200371

Gria4 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, AMPA4 (Gria4), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gria4
• Synonyms: GluA4; GluR-D; GluR4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200371 representing NM_017263
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATTATTTGCAGGCAGATTGTCTTGTTGTTTTCTGGATTTTGGGGACTCGCCATGGGAGCCTTTC
  CAAGCAGCGTTCAAATAGGTGGTCTCTTCATCCGAAACACAGACCAGGAATACACTGCTTTTAGACTGGC
  AATCTTTCTTCATAACACCAGCCCCAATGCATCGGAAGCTCCTTTCAATTTGGTACCTCATGTGGACAAC
  ATTGAGACTGCCAACAGTTTTGCTGTGACAAACGCCTTCTGTTCCCAGTATTCTAGAGGGGTGTTTGCCA
  TTTTTGGACTCTATGACAAGAGATCCGTGCATACCTTGACCTCGTTCTGCAGTGCTCTGCACATCTCTCT
  CATCACACCAAGCTTTCCCACTGAAGGGGAGAGCCAGTTTGTGCTGCAGCTAAGACCTTCACTGAGAGGT
  GCACTCCTGAGCCTCCTGGATCACTATGAGTGGAACTGTTTCGTCTTCCTGTATGATACAGACAGGGGGT
  ATTCAATACTTCAAGCTATAATGGAAAAAGCAGGACAAAATGGATGGCATGTCAGTGCAATATGTGTGGA
  AAATTTTAATGATGTCAGCTACAGGCAACTGCTAGAAGAGCTTGACAGAAGACAAGAGAAGAAATTTGTG
  ATAGATTGTGAGATAGAGAGGCTTCAAAACATTTTAGAACAAATTGTGAGTGTTGGGAAGCATGTCAAAG
  GCTACCATTATATCATCGCAAATTTGGGTTTCAAGGATATTTCTCTTGAGAGATTTATACATGGAGGAGC
  AAATGTAACAGGATTCCAGTTGGTAGATTTTAATACACCCATGGTAACCAAACTAATGGATCGGTGGAAG
  AAACTAGATCAGAGAGAATATCCAGGTTCTGAAACACCTCCAAAGTACACCTCTGCTCTCACTTATGATG
  GAGTCCTGGTGATGGCTGAAACTTTCCGAAGTCTCAGAAGACAGAAAATTGATATTTCAAGGAGAGGAAA
  TGCTGGGGACTGTCTGGCAAACCCTGCTGCTCCCTGGGGCCAGGGAATTGACATGGAGAGGACACTGAAG
  CAGGTTCGAATTCAAGGGCTGACTGGGAATGTTCAATTTGACCATTATGGACGTAGAGTTAATTACACAA
  TGGATGTGTTTGAACTGAAAAGCACAGGACCTCGAAAGGTTGGCTACTGGAATGATATGGATAAATTAGT
  CTTGATTCAAGATATGCCTACTCTTGGCAATGACACAGCAGCTATTGAGAACAGAACAGTGGTTGTAACC
  ACAATTATGGAATCTCCCTATGTTATGTACAAGAAAAATCATGAAATGTTTGAAGGAAATGACAAGTACG
  AAGGCTACTGTGTAGATCTGGCATCGGAAATTGCAAAACATATTGGTATCAAATATAAAATTGCCATTGT
  TCCTGATGGAAAATATGGAGCAAGGGACGCAGACACTAAGATCTGGAATGGGATGGTAGGAGAGCTTGTG
  TATGGGAAAGCAGAGATTGCTATTGCCCCTCTGACAATCACATTGGTTCGAGAGGAAGTCATCGATTTTT
  CTAAGCCTTTTATGAGTTTAGGCATCTCTATCATGATCAAAAAACCTCAGAAATCTAAACCAGGAGTCTT
  TTCCTTCTTGGACCCTCTGGCCTATGAGATCTGGATGTGCATAGTGTTTGCATACATTGGTGTCAGTGTG
  GTCTTGTTCCTAGTCAGTAGGTTTAGCCCATATGAGTGGCACACAGAAGAACCTGAGGATGGGAAGGAAG
  GACCCAGTGACCAGCCTCCCAATGAATTTGGCATCTTTAACAGCCTTTGGTTTTCCCTGGGTGCCTTTAT
  GCAACAAGGATGTGACATTTCACCCAGATCCCTGTCAGGTCGGATTGTTGGAGGCGTGTGGTGGTTCTTC
  ACACTCATCATTATATCGTCCTACACTGCTAATCTGGCTGCATTCCTTACTGTGGAGAGAATGGTCTCCC
  CCATAGAAAGTGCAGAAGACCTGGCCAAACAAACAGAAATTGCCTATGGAACACTTGATTCTGGGTCAAC
  AAAAGAATTCTTCAGAAGATCAAAAATAGCAGTGTATGAAAAGATGTGGACCTACATGCGATCGGCAGAG
  CCGTCTGTGTTCACTAGAACTACAGCTGAGGGCGTGGCTCGTGTCCGCAAGTCCAAGGGCAAATTTGCCT
  TTCTCCTGGAGTCCACGATGAATGAATACATTGAGCAGCGAAAGCCCTGTGACACGATGAAAGTGGGAGG
  AAACCTGGATTCCAAAGGCTATGGTGTAGCAACGCCCAAGGGTTCCTCATTAAGAACTCCTGTAAACCTT
  GCCGTTTTGAAACTCAGTGAGGCAGGCGTCTTAGACAAGCTGAAAAACAAATGGTGGTACGATAAAGGTG
  AATGTGGACCCAAGGACTCGGGAAGCAAGGACAAGACGAGTGCCTTGAGCCTGAGCAACGTAGCAGGCGT
  CTTCTACATTCTGGTTGGCGGCCTGGGCTTGGCAATGCTGGTGGCTTTGATAGAGTTCTGTTACAAGTCC
  AGGGCAGAGGCGAAGAGAATGAAGCTGACTTTTTCCGAAGCCATAAGAAACAAAGCCAGGTTATCCATCA
  CTGGGAGTGTGGGAGAAAACGGCCGTGTGCTGACCCCTGACTGCCCCAAGGCCGTACACACAGGAACTGC
  AATTAGACAAAGTTCGGGATTGGCTGTCATTGCATCGGACCTACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200371 representing NM_017263
  Red=Cloning site Green=Tags(s)
  
  MRIICRQIVLLFSGFWGLAMGAFPSSVQIGGLFIRNTDQEYTAFRLAIFLHNTSPNASEAPFNLVPHVDN
  IETANSFAVTNAFCSQYSRGVFAIFGLYDKRSVHTLTSFCSALHISLITPSFPTEGESQFVLQLRPSLRG
  ALLSLLDHYEWNCFVFLYDTDRGYSILQAIMEKAGQNGWHVSAICVENFNDVSYRQLLEELDRRQEKKFV
  IDCEIERLQNILEQIVSVGKHVKGYHYIIANLGFKDISLERFIHGGANVTGFQLVDFNTPMVTKLMDRWK
  KLDQREYPGSETPPKYTSALTYDGVLVMAETFRSLRRQKIDISRRGNAGDCLANPAAPWGQGIDMERTLK
  QVRIQGLTGNVQFDHYGRRVNYTMDVFELKSTGPRKVGYWNDMDKLVLIQDMPTLGNDTAAIENRTVVVT
  TIMESPYVMYKKNHEMFEGNDKYEGYCVDLASEIAKHIGIKYKIAIVPDGKYGARDADTKIWNGMVGELV
  YGKAEIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSV
  VLFLVSRFSPYEWHTEEPEDGKEGPSDQPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFF
  TLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYEKMWTYMRSAE
  PSVFTRTTAEGVARVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPKGSSLRTPVNL
  AVLKLSEAGVLDKLKNKWWYDKGECGPKDSGSKDKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKS
  RAEAKRMKLTFSEAIRNKARLSITGSVGENGRVLTPDCPKAVHTGTAIRQSSGLAVIASDLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017263
• ORF Size: 2706 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017263.1, NM_017263.2, NP_058959.2
• RefSeq Size: 4141
• RefSeq ORF: 2709
• Locus ID: 29629
• MW: 100.9 kDa
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008]