Pparg (NM_001145366) Rat Tagged ORF Clone

CAT#: RR200399

Pparg (Myc-DDK-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pparg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200399 representing NM_001145366
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTTCGGAATCAGCTCTGTGGACCTCTCTGTGATGG
  ATGACCACTCCCATTCCTTTGACATCAAACCCTTTACCACGGTTGATTTCTCCAGCATTTCTGCTCCACA
  CTATGAAGACATCCCGTTCACAAGAGCTGACCCAATGGTTGCTGATTACAAATATGACCTGAAGCTCCAA
  GAATACCAAAGTGCGATCAAAGTAGAGCCTGCGTCCCCGCCTTATTATTCTGAAAAAACCCAACTCTACA
  ACAGGCCACATGAAGAGCCTTCAAACTCCCTCATGGCCATCGAGTGCCGAGTCTGTGGGGATAAAGCATC
  AGGCTTCCACTATGGAGTCCATGCTTGTGAAGGATGCAAGGGTTTTTTCCGAAGAACCATCCGATTGAAG
  CTTATTTATGATAGGTGTGATCTTAACTGTCGGATCCACAAAAAGAGTAGAAATAAATGTCAGTACTGTC
  GGTTTCAGAAGTGCCTTGCTGTGGGGATGTCTCACAATGCCATCAGGTTTGGGCGAATGCCACAGGCCGA
  GAAGGAGAAGCTGTTGGCGGAGATCTCCAGTGATATCGACCAGCTGAACCCAGAGTCTGCTGATCTGCGA
  GCCCTGGCAAAGCATTTGTATGACTCATACATAAAGTCCTTCCCGCTGACCAAAGCCAAGGCGAGGGCGA
  TCTTGACAGGAAAGACAACAGACAAATCACCATTTGTCATCTACGACATGAATTCCTTAATGATGGGAGA
  AGACAAAATCAAGTTCAAACATATCACCCCCCTGCAGGAGCAGAGCAAAGAGGTGGCCATCCGCATTTTT
  CAAGGGTGCCAGTTTCGATCCGTGGAAGCTGTGCAAGAGATCACAGAGTATGCCAAAAATATCCCTGGTT
  TCATTAACCTTGACTTGAATGACCAAGTGACTCTGCTCAAGTATGGTGTCCATGAGATCATCTACACCAT
  GCTGGCCTCCCTGATGAATAAAGATGGAGTCCTCATATCAGAGGGACAAGGATTCATGACCAGGGAGTTC
  CTCAAAAGCCTGCGGAAGCCCTTTGGTGACTTTATGGAGCCTAAGTTTGAGTTTGCTGTGAAGTTCAATG
  CACTGGAATTAGATGACAGTGACTTGGCCATATTTATAGCTGTCATTATTCTCAGTGGAGACCGCCCAGG
  CTTGCTGAACGTGAAGCCCATCGAGGACATCCAAGACAACCTGCTGCAGGCCCTGGAACTCCAGCTGAAG
  CTGAACCACCCGGAGTCCTCCCAGCTGTTCGCCAAGGTGCTCCAGAAGATGACAGACCTCAGGCAGATTG
  TCACAGAGCACGTGCAGCTACTGCATGTGATCAAGAAGACGGAGACAGATATGAGCCTTCACCCTCTGCT
  CCAGGAGATCTACAAGGACTTGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200399 representing NM_001145366
  Red=Cloning site Green=Tags(s)
  
  MVDTEMPFWPTNFGISSVDLSVMDDHSHSFDIKPFTTVDFSSISAPHYEDIPFTRADPMVADYKYDLKLQ
  EYQSAIKVEPASPPYYSEKTQLYNRPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLK
  LIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESADLR
  ALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIF
  QGCQFRSVEAVQEITEYAKNIPGFINLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREF
  LKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLK
  LNHPESSQLFAKVLQKMTDLRQIVTEHVQLLHVIKKTETDMSLHPLLQEIYKDLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145366
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145366.1, NP_001138838.1
• RefSeq Size: 1830
• RefSeq ORF: 1428
• Locus ID: 25664
• MW: 54.5 kDa
• Gene Summary: ligand-activated transcription factor; mediates expression of genes involved in lipid metabolism [RGD, Feb 2006]