Adarb1 (NM_012894) Rat Tagged ORF Clone

CAT#: RR200427

Adarb1 (Myc-DDK-tagged ORF) - Rat adenosine deaminase, RNA-specific, B1 (Adarb1), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adarb1
• Synonyms: Adar2; Red1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200427 representing NM_012894
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATATAGAAGACGAAGAGAATATGAGTTCCAGCAGCACTGATGTTAAAGAAAACCGCAATCTGGACA
  ACATGCCCCCCAAGGACAGCAGCACACCCGGGCCTGGCGAGGGTATTCCGCTCTCCAACGGGGGTGGTGG
  GAGCACCAGCAGGAAGCGGCCCCTGGAGGAGGGCAGCAATGGCCACTCCAAGTACCGCCTGAAGAAGCGA
  AGGAAAACGCCAGGGCCCGTTCTACCCAAGAACGCCCTGATGCAGCTGAACGAGATCAAACCGGGCTTAC
  AGTACATGCTGCTGTCCCAGACAGGACCCGTGCACGCACCTCTGTTTGTCATGTCTGTGGAGGTAAACGG
  GCAGGTCTTTGAAGGCTCCGGCCCTACAAAGAAGAAGGCAAAGCTGCATGCTGCTGAGAAGGCCCTGCGG
  TCTTTTGTCCAGTTTCCCAACGCCTCTGAGGCCCACCTGGCCATGGGAAGGACCCTCTCCGTGAACACAG
  ACTTCACGTCCGACCAGGCGGACTTCCCCGACACGCTCTTCAATGGCTTTGAGACTCCAGACAAGTCGGA
  GCCACCCTTCTACGTAGGCTCCAATGGGGATGACTCCTTCAGCTCAAGCGGAGACGTTAGCCTGTCAGCC
  TCCCCAGTGCCTGCCAGCCTTACCCAGCCTCCTCTGCCCATCCCACCACCATTCCCACCCCCAAGTGGGA
  AGAACCCCGTGATGATCTTGAATGAGCTGCGCCCAGGGCTGAAGTATGACTTCCTCTCCGAGAGTGGGGA
  GAGCCACGCCAAGAGCTTTGTCATGTCCGTGGTGGTAGATGGCCAGTTCTTTGAGGGCTCAGGGAGAAAC
  AAGAAGCTTGCCAAGGCCCGGGCTGCACAGTCTGCCTTGGCTACTGTCTTCAATTTGCACTTGGACCAAA
  CGCCATCTCGCCAGCCTGTCCTCAGTGAGGGTCTCCAGTTGCATTTGCCACAGGTATTGGCAGATGCTGT
  CTCACGCCTGGTCCTGGGTAAGTTCAGTGACCTGACAGACAACTTTTCCTCCCCTCACGCACGAAGAAAA
  GTGCTCTCTGGAGTAGTGATGACCACAGGTACAGATGTCAAAGATGCCAAGGTGATAAGTGTTTCGACAG
  GGACGAAGTGCATCAACGGCGAATACATGAGTGACCGTGGCCTGGCTCTCAATGACTGCCACGCAGAGAT
  AATCTCCCGAAGGTCCCTGCTCAGGTTTCTATACGCACAGCTCGAGCTTTACTTAAATAACAAAGAAGAC
  CAGAAAAAGTCCATATTTCAGAAGTCAGAGCGGGGTGGGTTCCGGCTGAAGGATACCGTGCAGTTCCACC
  TGTACATCAGCACCTCACCCTGCGGAGACGCCAGAATATTCTCTCCCCATGAGCCCGTGCTAGAGGGTAT
  GGCGCCAGACTCCCACCAGCTGACAGAACCGGCTGATAGACATCCGAATCGCAAAGCAAGGGGACAGCTG
  CGGACTAAAATAGAATCTGGCGAGGGGACAATCCCTGTGCGCTCAAATGCCAGCATCCAGACCTGGGATG
  GGGTGCTGCAGGGGGAACGGCTGCTCACCATGTCCTGCAGTGACAAGATAGCACGCTGGAACGTGGTGGG
  CATCCAGGGGGCCCTGCTCAGCATTTTCGTGGAGCCCATCTACTTCTCCAGCATCATCCTGGGCAGCCTG
  TACCACGGGGACCACCTCTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCGCTCT
  ACACCCTCAACAAGCCCCTGCTCAGCGGTATCAGCAATGCAGAGGCACGGCAGCCAGGGAAGGCACCCAA
  CTTCAGTGTCAACTGGACGGTGGGCGACACGGCCATTGAGGTCATCAATGCCACAACAGGGAAGGATGAG
  CTAGGCCGCCCCTCCCGCCTGTGTAAGCACGCGCTGTACTGTCGCTGGATGCGGGTACACGGAAAGGTGC
  CCCCCCACCTGCTGCGCACCAAGATCACCAAGCCCACCACCTACCACGAGTCCAAGCTGGCAGCGAAGGA
  GTACCAGGCTGCCAAGGCACGTCTGTTCACTGCCTTCATCAAGGCGGGGCTGGGCGCCTGGGTGGAGAAG
  CCCACAGAGCAGGACCAGTTCTCCTTCACTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200427 representing NM_012894
  Red=Cloning site Green=Tags(s)
  
  MDIEDEENMSSSSTDVKENRNLDNMPPKDSSTPGPGEGIPLSNGGGGSTSRKRPLEEGSNGHSKYRLKKR
  RKTPGPVLPKNALMQLNEIKPGLQYMLLSQTGPVHAPLFVMSVEVNGQVFEGSGPTKKKAKLHAAEKALR
  SFVQFPNASEAHLAMGRTLSVNTDFTSDQADFPDTLFNGFETPDKSEPPFYVGSNGDDSFSSSGDVSLSA
  SPVPASLTQPPLPIPPPFPPPSGKNPVMILNELRPGLKYDFLSESGESHAKSFVMSVVVDGQFFEGSGRN
  KKLAKARAAQSALATVFNLHLDQTPSRQPVLSEGLQLHLPQVLADAVSRLVLGKFSDLTDNFSSPHARRK
  VLSGVVMTTGTDVKDAKVISVSTGTKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYAQLELYLNNKED
  QKKSIFQKSERGGFRLKDTVQFHLYISTSPCGDARIFSPHEPVLEGMAPDSHQLTEPADRHPNRKARGQL
  RTKIESGEGTIPVRSNASIQTWDGVLQGERLLTMSCSDKIARWNVVGIQGALLSIFVEPIYFSSIILGSL
  YHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGKAPNFSVNWTVGDTAIEVINATTGKDE
  LGRPSRLCKHALYCRWMRVHGKVPPHLLRTKITKPTTYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEK
  PTEQDQFSFTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012894
• ORF Size: 2133 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012894.1, NM_012894.2, NP_037026.2
• RefSeq Size: 6412
• RefSeq ORF: 2136
• Locus ID: 25367
• MW: 77.9 kDa
• Gene Summary: This gene encodes a double-stranded-RNA-specific adenosine deaminase that is involved in editing pre-mRNAs by site-specific conversion of adenosine (A) to inosine (I). Substrates for this enzyme include ionotropic glutamate receptors (GluR2-6) and serotonin receptor (5HT2C). Studies in rodents have shown that this protein can modify its own pre-mRNA by A->I editing to create a novel acceptor splice site, alternative splicing to which results in down regulation of its protein expression. Additional splicing events result in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]