Stat1 (NM_001034164) Rat Tagged ORF Clone

CAT#: RR200448

Stat1 (Myc-DDK-tagged ORF) - Rat signal transducer and activator of transcription 1 (Stat1), transcript variant beta, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stat1
• Synonyms: DD6G4-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200448 representing NM_001034164
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCAGTGGTACGAGCTTCAGCAGCTGGACTCCAAGTTCCTGGAGCAGGTGCACCAGCTGTACGATG
  ACAGTTTCCCCATGGAAATCAGACAGTACCTGGCCCAGTGGCTAGAAAAGCAAGACTGGGAGCATGCTGC
  CTACGACGTCTCGTTCGCCACCATCCGCTTCCATGACCTGCTCTCCCAGCTGGATGACCAGTACAGCCGC
  TTTTCTTTGGAGAATAATTTCTTGTTGCAGCACAACATAAGGAAAAGCAAGCGTAATCTCCAGGATAACT
  TCCAAGAAGATCCCGTACAGATGTCCATGATCATCCACAACTGTCTGAAGGAGGAGAGGAAGATTTTGGA
  AAACGCCCAGCGATTTAATCAGGCCCAGGAGGGAAATATTCAGAACACCGTGATGTTAGATAAACAGAAG
  GAGCTGGACAGTAAAGTCAGAAATGTGAAGGACCAAGTCATGTGCATAGAGCATGAAATCAAGACCCTAG
  AAGACTTACAAGATGAATATGACTTTAAATGCAAAACCTCTCAGAACAGAGAAAGCGAAGCCAATGGTGT
  GGCGAAGAGCGACCAGAAACAGGAACAGCTGCTGCTGCATAAGATGTTCTTAATGCTTGACAACAAGAGA
  AAGGAGATAATTCACAAAATCAGAGAGCTGCTGAACTCCATCGAGCTCACTCAGAACACTCTGATTAATG
  ATGAGCTTGTGGAGTGGAAGCGAAGACAGCAGAGTGCCTGCATTGGGGGACCGCCCAATGCCTGCCTGGA
  TCAGCTGCAAAGCTGGTTCACCATTGTTGCAGAGAGCCTGCAGCAGGTCCGTCAGCAGCTTAAAAAGCTG
  GAGGAGTTGGAACAGAAATTCACCTATGAGCAGGACCCTATTACAAAAAACAAGCAGGTGTTATCCGATC
  GCACCTTCGTCCTCTTCCAGCAGCTCATTCAGAGCTCCTTTGTGGTGGAACGACAGCCGTGCATGCCAAC
  TCACCCTCAGAGGCCCCTGGTCTTGAAGACTGGGGTACAGTTCACTGTGAAGCTGAGACTGTTGGTGAAA
  TTGCAAGAACTGAACTATAACTTGAAAGTGAAAGTCTCATTTGACAAAGATGTGAACGAGAAAAACACAG
  TGAAAGGATTCCGGAAGTTCAACATCTTGGGTACTCACACAAAAGTGATGAACATGGAAGAATCCACCAA
  CGGAAGTTTGGCAGCTGAGTTCCGACACCTGCAACTGAAGGAACAGAAAAATGCTGGGAACAGAACCAAT
  GAGGGTCCTCTCATTGTCACCGAAGAACTTCACTCTCTTAGCTTTGAAACCCAGTTGTGCCAGCCAGGCT
  TGGTGATTGACCTTGAGACCACCTCTCTTCCCGTCGTGGTGATCTCCAATGTCAGCCAGCTCCCCAGTGG
  CTGGGCTTCTATCCTGTGGTACAACATGCTGGTGACAGAGCCCAGGAATCTCTCCTTCTTCCTGAACCCC
  CCGTGTGCGTGGTGGTCCCAGCTCTCAGAGGTGCTGAGTTGGCAGTTTTCGTCAGTCACCAAGAGAGGTC
  TCAACGCTGACCAGCTGAGCATGCTGGGGGAGAAGCTTCTGGGCCCTAATGCTGGCCCTGATGGTCTTAT
  TCCATGGACAAGGTTCTGTAAGGAAAATATTAACGATAAGAATTTCTCCTTCTGGCCTTGGATTGACACC
  ATCCTAGAACTCATTAAGAAGCACCTGCTTTGCCTCTGGAATGATGGGTGCATCATGGGCTTTATCAGCA
  AGGAGCGAGAACGTGCTCTGCTCAAGGACCAGCAGCCCGGGACGTTCCTGCTTAGGTTCAGTGAGAGCTC
  CCGGGAAGGGGCCATCACATTCACATGGGTGGAACGGTCCCAAAATGGAGGTGAACCCGACTTCCATGCT
  GTTGAGCCCTACACGAAGAAAGAACTTTCAGCTGTTACTTTCCCAGATATTATTCGCAATTACAAAGTCA
  TGGCTGCTGAGAATATACCAGAGAATCCCCTGAAGTATCTGTATCCCAATATTGACAAAGACCATGCCTT
  CGGGAAGTACTACTCCAGGCCAAAGGAAGCACCAGAACCGATGGAGCTCGATGACCCTAAGAGAACTGGA
  TACATCAAGACTGAGTTGATTTCTGTGTCTGAAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200448 representing NM_001034164
  Red=Cloning site Green=Tags(s)
  
  MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAAYDVSFATIRFHDLLSQLDDQYSR
  FSLENNFLLQHNIRKSKRNLQDNFQEDPVQMSMIIHNCLKEERKILENAQRFNQAQEGNIQNTVMLDKQK
  ELDSKVRNVKDQVMCIEHEIKTLEDLQDEYDFKCKTSQNRESEANGVAKSDQKQEQLLLHKMFLMLDNKR
  KEIIHKIRELLNSIELTQNTLINDELVEWKRRQQSACIGGPPNACLDQLQSWFTIVAESLQQVRQQLKKL
  EELEQKFTYEQDPITKNKQVLSDRTFVLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVK
  LQELNYNLKVKVSFDKDVNEKNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGNRTN
  EGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVTEPRNLSFFLNP
  PCAWWSQLSEVLSWQFSSVTKRGLNADQLSMLGEKLLGPNAGPDGLIPWTRFCKENINDKNFSFWPWIDT
  ILELIKKHLLCLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHA
  VEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDDPKRTG
  YIKTELISVSEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034164
• ORF Size: 2136 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034164.1, NP_001029336.1
• RefSeq Size: 2772 bp
• RefSeq ORF: 2139 bp
• Locus ID: 25124
• Cytogenetics: 9q22
• MW: 83.1 kDa
• Gene Summary: component of the IFN-gamma receptor signaling pathway and other signaling pathways; plays a role in development of cytokine resistance [RGD, Feb 2006]