Pfkfb2 (NM_080477) Rat Tagged ORF Clone

CAT#: RR200496

Pfkfb2 (Myc-DDK-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 3, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfkfb2
• Synonyms: PFK-2; RH2K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200496 representing NM_080477
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGAATAGTACATTTTCCACAGAAGACAGCAGCAGCAGCAGCTATAAACCCCACGCCTCAAACC
  TCCGAAGGGCAGGGAAGAAATGCTCATGGGCTTCCTACATGACCAACTCCCCAACACTCATTGTTATGAT
  TGGCTTGCCAGCCCGGGGTAAGACTTATGTGTCCAAGAAATTAACACGCTACCTGAACTGGATTGGAGTA
  CCTACCAAAGTGTTTAATCTTGGTGTATATCGACGGGAAGCAGTCAAGTCCTATAAGTCCTATGACTTCT
  TTCGACATGACAATGAAGAGGCCATGAAGATCCGCAAACAGTGTGCCCTGGTGGCACTGGAAGATGTGAA
  GGCCTACTTTACTGAAGAGAGTGGGCAGATCGCGGTGTTTGATGCCACCAATACCACTCGGGAGAGGAGG
  GACATGATTTTGAACTTTGCCAAGCAGAATGCCTTCAAGGTATTCTTTGTGGAATCTGTGTGTGATGATC
  CTGATGTCATTGCTGCCAATATTCTGGAGGTAAAAGTGTCAAGCCCTGACTACCCCGAAAGGAATAGGGA
  GAATGTGATGGAGGACTTCCTGAAGAGAATTGAGTGCTACAAGGTCACTTACCAGCCCCTTGACCCAGAC
  AACTATGATAAGGACCTCTCGTTCATAAAGGTGATGAATGTAGGCCAGAGGTTTCTGGTCAACAGAGTTC
  AGGACTACATCCAGAGTAAGATTGTCTACTACCTGATGAACATCCATGTCCATCCTCGCACCATCTATCT
  GTGCCGGCACGGAGAGAGCGAATTCAATCTTTTGGGAAAGATTGGGGGTGACTCTGGCCTTTCGTTGCGA
  GGAAAGCAGTTTGCTCAGGCTCTGAAGAAGTTTCTGGAGGAACAGGAGATCCAGGACCTCAAAGTGTGGA
  CAAGCCAGTTGAAGAGGACAATTCAGACTGCTGAGTCTCTTGGGGTGACCTATGAGCAATGGAAGATCCT
  AAATGAGATTGATGCTGGCGTGTGTGAGGAGATGACTTATTCGGAGATCGAACAACGGTATCCAGAGGAA
  TTTGCACTTCGAGATCAAGAGAAGTATCTGTATCGATATCCTGGTGGGGAGTCATACCAGGACCTGGTGC
  AGCGGCTGGAGCCTGTGATCATGGAGCTGGAGCGGCAAGGCAACGTCCTCGTTATCTCTCACCAGGCTGT
  CATGCGCTGCCTCCTGGCCTACTTCTTGGATAAAGGCGCAGATGAGTTGCCGTACTTGAGGTGCCCCCTC
  CACATCATCTTCAAACTTACTCCTGTGGCCTATGGTTGCAAAGTGGAAACAATTACACTGAATGTGGAAG
  CTGTGGACACACATCGTGACAAGCCAACTGAAGTGGAGAATGTGCTGGCTAAGCATAGACGCCCCTCAAT
  GGCATCCCTCACTTTGCTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200496 representing NM_080477
  Red=Cloning site Green=Tags(s)
  
  MSENSTFSTEDSSSSSYKPHASNLRRAGKKCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGV
  PTKVFNLGVYRREAVKSYKSYDFFRHDNEEAMKIRKQCALVALEDVKAYFTEESGQIAVFDATNTTRERR
  DMILNFAKQNAFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYQPLDPD
  NYDKDLSFIKVMNVGQRFLVNRVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSGLSLR
  GKQFAQALKKFLEEQEIQDLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGVCEEMTYSEIEQRYPEE
  FALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNVLVISHQAVMRCLLAYFLDKGADELPYLRCPL
  HIIFKLTPVAYGCKVETITLNVEAVDTHRDKPTEVENVLAKHRRPSMASLTLLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080477
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_080477.2, NM_080477.3, NP_536725.2
• RefSeq Size: 3575
• RefSeq ORF: 1425
• Locus ID: 24640
• MW: 54.8 kDa
• Gene Summary: catalyzes the synthesis and degradation of fructose 2,6-bisphosphate [RGD, Feb 2006]