Pde4d (NM_001113332) Rat Tagged ORF Clone

CAT#: RR200501

Pde4d (Myc-DDK-tagged ORF) - Rat phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (Pde4d), transcript variant 3, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde4d
• Synonyms: DPDE3; PDE3/IVd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200501 representing NM_001113332
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGCAGACGACAAGCCCGGACACCCTTACAGTCCCTGAAGTGGATAATCCGCATGTTCCAAACC
  CATGGCTCAATGAGGATCTTGTGAAATCCCTGCGGGAAAACCTGTTGCAGCACGAAAAGTCAAAGACAGC
  GAGGAAATCCGTTTCTCCCAAGCTCTCCCCGGTGATTTCTCCCAGGAATTCCCCCAGGCTCCTGCGCAGG
  ATGCTTCTGAGTAGCAACATCCCCAAGCAGCGTCGCTTCACAGTGGCACATACATGTTTCGATGTGGACA
  ATGGCACATCAGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGGTCTGGGCTAATTCTCCAAGC
  AAACTTTGTGCACAGTCAACGCCGGGAGTCCTTCCTGTATCGATCAGACAGCGATTATGACCTCTCTCCA
  AAGTCTATGTCCAGGAACTCCTCGATTGCCAGTGATATACATGGAGATGACTTGATTGTGACTCCATTTG
  CTCAGGTCTTGGCCAGCCTTCGAACTGTAAGAAACAACTTTGCTGCATTAACTAATTTGCAAGATCGCGC
  ACCCAGCAAAAGATCACCCATGTGCAACCAACCATCCATCAACAAAGCCACCATCACAGAGGAGGCCTAC
  CAGAAACTGGCCAGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTGCAGACCA
  GGCACTCCGTCAGTGAGATGGCCTCCAACAAGTTCAAGAGGATGCTTAATCGGGAGCTCACCCACCTCTC
  TGAAATGAGTCGGTCTGGCAACCAGGTGTCGGAGTACATATCAAACACGTTCTTAGATAAGCAACATGAA
  GTAGAAATTCCCTCTCCGACTCAGAAGGAAAAAGAGAAGAAGAAAAGGCCGATGTCACAGATCAGTGGGG
  TCAAGAAGTTGATGCACAGCTCCAGCCTGACCAATTCCTGCATTCCAAGATTTGGGGTTAAAACAGAGCA
  GGAAGATGTCCTGGCCAAGGAACTAGAAGACGTGAACAAGTGGGGCCTCCACGTTTTCCGAATAGCGGAG
  CTGTCTGGCAACCGGCCTCTGACTGTTATCATGCACACCATTTTTCAGGAACGAGATTTGTTAAAAACGT
  TTAAAATCCCAGTGGACACTTTGATTACGTATCTTATGACTCTAGAAGACCATTACCATGCTGACGTGGC
  CTATCACAACAACATCCATGCTGCAGATGTCGTCCAGTCAACTCATGTGCTGCTCTCTACACCCGCTTTG
  GAGGCTGTTTTCACTGACTTGGAGATTCTCGCGGCCATTTTTGCCAGTGCAATACATGATGTGGATCATC
  CTGGTGTGTCAAATCAATTTCTGATCAATACAAACTCGGAACTTGCCTTGATGTACAACGACTCCTCCGT
  CTTAGAGAATCATCATTTGGCTGTGGGCTTTAAGTTGCTCCAGGAAGAAAACTGTGACATTTTCCAGAAT
  CTGACCAAAAAGCAAAGACAATCTTTAAGGAAAATGGCCATTGACATTGTACTAGCGACAGACATGTCAA
  AGCACATGAATCTGCTGGCTGATCTGAAAACAATGGTTGAAACGAAGAAGGTGACGAGCTCTGGCGTCCT
  CCTCCTTGATAACTATTCTGACAGGATCCAGGTCCTCCAGAATATGGTGCACTGTGCAGACCTGAGCAAC
  CCCACAAAGCCACTCCAGCTCTACCGCCAGTGGACGGACCGGATAATGGAGGAGTTCTTCCGTCAGGGGG
  ACCGGGAGCGTGAGCGTGGCATGGAGATAAGTCCCATGTGTGACAAGCACAACGCCTCTGTGGAAAAATC
  ACAGGTGGGCTTCATAGACTACATTGTTCATCCACTCTGGGAGACATGGGCAGACCTCGTACATCCCGAC
  GCCCAGGACATTTTGGACACTTTGGAGGACAATCGTGAGTGGTACCAGAGCACAATCCCCCAGAGCCCCT
  CCCCTGCACCTGATGACCAAGAGGACGGCCGTCAGGGACAGACTGAAAAATTCCAGTTCGAACTAACCTT
  AGAGGAAGATGGCGAGTCAGACACTGAAAAGGACAGTGGAAGTCAAGTGGAGGAAGACACTAGCTGCAGT
  GACTCTAAGACTCTGTGCACCCAAGACTCAGAGTCCACTGAAATTCCCCTTGACGAGCAGGTTGAAGAGG
  AGGCTGTAGCAGAAGAGGAAAGCCAGCCCCAAACTGGCGTCGCAGACGATTGTTGTCCTGACACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200501 representing NM_001113332
  Red=Cloning site Green=Tags(s)
  
  MAQQTTSPDTLTVPEVDNPHVPNPWLNEDLVKSLRENLLQHEKSKTARKSVSPKLSPVISPRNSPRLLRR
  MLLSSNIPKQRRFTVAHTCFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSP
  KSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAY
  QKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHE
  VEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAE
  LSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPAL
  EAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQN
  LTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSN
  PTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPD
  AQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCS
  DSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113332
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001113332.1, NP_001106803.1
• RefSeq Size: 2322
• RefSeq ORF: 2238
• Locus ID: 24627
• MW: 84.3 kDa
• Gene Summary: may contain eight isoforms that display differential expression and activities; isorofms Pde4D6 and Pde4D7 exhibit high affinity for cyclic AMP and are inhibited by rolipram [RGD, Feb 2006]