Mmp13 (NM_133530) Rat Tagged ORF Clone

CAT#: RR200578

Mmp13 (Myc-DDK-tagged ORF) - Rat matrix metallopeptidase 13 (Mmp13), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mmp13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200578 representing NM_133530
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTCAGCTATTCTGGCCACCTTCTTCTTGTTGAGTTGGACTCACTGTTGGTCCCTGCCCCTTCCCT
  ATGGTGATGATGATGATGATGACTTGTCTGAGGAAGATCTTGAGTTTGCAGAGCACTACTTGAAATCATA
  CTACCATCCTGTGACTCTTGCGGGAATCCTGAAGAAGTCTACAGTGACCTCTACAGTTGATAGACTCCGA
  GAAATGCAGTCTTTCTTTGGCTTAGATGTGACTGGCAAACTTGATGATCCTACCTTAGACATCATGAGAA
  AACCAAGATGTGGAGTGCCTGATGTGGGTGAATACAATGTTTTCCCTCGAACACTCAAATGGTCCCAAAC
  AAACTTAACTTACAGAATTGTGAACTACACCCCTGATATATCCCATTCTGAAGTGGAGAAGGCCTTCAGA
  AAAGCCTTCAAGGTCTGGTCTGATGTGACACCTCTGAATTTTACCAGGATCCATGATGGCACTGCTGATA
  TCATGATATCTTTTGGAACTAAAGAACATGGTGACTTCTACCCATTTGATGGGCCTTCTGGTCTTCTGGC
  ACACGCTTTTCCTCCTGGACCAAACCTTGGCGGGGACGCCCATTTTGATGATGATGAAACCTGGACAAGC
  AGCTCCAAAGGCTACAACTTATTTATTGTTGCTGCCCATGAGCTTGGCCACTCCCTCGGTCTGGACCACT
  CCAAGGACCCTGGAGCCCTGATGTTTCCCATCTATACCTACACTGGCAAAAGCCATTTCATGCTCCCAGA
  TGATGACGTTCAAGGAATCCAGTCTCTCTATGGTCCAGGAGATGAAGACCCCAACCCTAAGCACCCCAAA
  ACACCAGAGAAGTGTGACCCAGCCCTATCCCTTGATGCCATTACTAGTCTCCGAGGAGAAACCATGATCT
  TTAAAGACAGATTCTTCTGGCGTCTGCACCCTCAGCAGGTTGAGCCTGAACTGTTTTTGACCAAGTCCTT
  TTGGCCAGAACTTCCCAACCATGTGGATGCTGCATACGAGCATCCATCCCGAGACCTCATGTTCATCTTT
  AGAGGGAGAAAATTCTGGGCCCTGAATGGGTATGACATTATGGAGGGTTATCCCAGAAAAATATCTGACC
  TGGGATTTCCAAAAGAGGTGAAAAGGCTCAGTGCTGCGGTTCACTTTGAGGACACGGGGAAGACCCTCTT
  CTTCTCAGGGAACCACGTGTGGAGTTATGATGATGCTAACCAGACTATGGACAAAGACTATCCCCGCCTC
  ATAGAAGAAGAATTCCCTGGAATTGGCGACAAAGTAGATGCTGTCTATGAGAAAAATGGCTATATCTACT
  TTTTCAATGGGCCCATACAGTTTGAATACAGTATCTGGAGTAATCGCATTGTGAGAGTCATGCCAACAAA
  TTCCTTATTGTGGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200578 representing NM_133530
  Red=Cloning site Green=Tags(s)
  
  MHSAILATFFLLSWTHCWSLPLPYGDDDDDDLSEEDLEFAEHYLKSYYHPVTLAGILKKSTVTSTVDRLR
  EMQSFFGLDVTGKLDDPTLDIMRKPRCGVPDVGEYNVFPRTLKWSQTNLTYRIVNYTPDISHSEVEKAFR
  KAFKVWSDVTPLNFTRIHDGTADIMISFGTKEHGDFYPFDGPSGLLAHAFPPGPNLGGDAHFDDDETWTS
  SSKGYNLFIVAAHELGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPK
  TPEKCDPALSLDAITSLRGETMIFKDRFFWRLHPQQVEPELFLTKSFWPELPNHVDAAYEHPSRDLMFIF
  RGRKFWALNGYDIMEGYPRKISDLGFPKEVKRLSAAVHFEDTGKTLFFSGNHVWSYDDANQTMDKDYPRL
  IEEEFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPTNSLLWC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133530
• ORF Size: 1416 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133530.1, NP_598214.1
• RefSeq Size: 2602
• RefSeq ORF: 1419
• Locus ID: 171052
• MW: 54.1 kDa
• Gene Summary: enzyme that degrades collagen type I [RGD, Feb 2006]