Oprk1 (NM_017167) Rat Tagged ORF Clone

CAT#: RR200637

Oprk1 (Myc-DDK-tagged ORF) - Rat opioid receptor, kappa 1 (Oprk1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Oprk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200637 representing NM_017167
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCCCCATCCAGATTTTCCGCGGAGAGCCAGGCCCTACCTGTGCTCCCAGTGCTTGCCTACTCC
  CCAACAGCAGCTCTTGGTTCCCCAACTGGGCCGAATCGGACAGCAATGGCAGTGTGGGCTCCGAGGACCA
  GCAGCTGGAGCCCGCGCACATCTCTCCAGCCATCCCTGTTATCATCACCGCTGTCTACTCTGTGGTGTTT
  GTGGTGGGCTTAGTGGGCAATTCCCTGGTCATGTTTGTCATCATCCGATACACAAAGATGAAGACCGCAA
  CCAACATCTACATATTTAACCTGGCTTTGGCAGATGCTTTGGTTACTACCACTATGCCCTTCCAGAGTGC
  TGTCTACTTGATGAATTCTTGGCCTTTTGGAGATGTTCTGTGCAAGATTGTCATTTCCATTGACTACTAC
  AACATGTTTACCAGCATATTCACCTTGACCATGATGAGTGTGGACCGCTACATTGCCGTGTGCCACCCTG
  TGAAAGCTTTGGATTTCCGAACACCTTTGAAAGCAAAGATCATCAACATCTGCATTTGGCTACTGGCATC
  ATCTGTTGGTATATCAGCGATAGTCCTTGGAGGCACCAAAGTCAGGGAAGATGTGGATGTCATTGAATGC
  TCCTTGCAGTTTCCTGATGATGAATATTCCTGGTGGGACCTCTTCATGAAGATCTGTGTCTTCGTCTTTG
  CCTTTGTTATCCCTGTCTTAATCATCATTGTCTGCTACACCCTGATGATCCTGCGCTTGAAGAGTGTCCG
  GCTCCTCTCGGGCTCTCGAGAGAAGGACCGAAATCTCCGCCGGATCACCAAGCTGGTGCTGGTAGTGGTT
  GCAGTCTTCATCATCTGTTGGACCCCCATCCACATCTTTATCCTGGTCGAGGCTCTAGGCAGCACCTCCC
  ACAGCACAGCTGTCCTCTCTAGCTATTACTTCTGCATTGCCTTGGGTTATACCAACAGCAGCTTGAATCC
  TGTTCTCTATGCCTTTCTTGATGAAAACTTCAAGCGGTGTTTTAGGGACTTCTGCTTCCCCATTAAGATG
  CGAATGGAGCGCCAGAGCACAAACAGAGTTAGAAACACAGTTCAGGATCCTGCTTCCATGAGGGATGTGG
  GTGGGATGAATAAGCCAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200637 representing NM_017167
  Red=Cloning site Green=Tags(s)
  
  MESPIQIFRGEPGPTCAPSACLLPNSSSWFPNWAESDSNGSVGSEDQQLEPAHISPAIPVIITAVYSVVF
  VVGLVGNSLVMFVIIRYTKMKTATNIYIFNLALADALVTTTMPFQSAVYLMNSWPFGDVLCKIVISIDYY
  NMFTSIFTLTMMSVDRYIAVCHPVKALDFRTPLKAKIINICIWLLASSVGISAIVLGGTKVREDVDVIEC
  SLQFPDDEYSWWDLFMKICVFVFAFVIPVLIIIVCYTLMILRLKSVRLLSGSREKDRNLRRITKLVLVVV
  AVFIICWTPIHIFILVEALGSTSHSTAVLSSYYFCIALGYTNSSLNPVLYAFLDENFKRCFRDFCFPIKM
  RMERQSTNRVRNTVQDPASMRDVGGMNKPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017167
• ORF Size: 1140 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017167.1, NM_017167.2, NM_017167.3, NP_058863.1
• RefSeq Size: 1358
• RefSeq ORF: 1143
• Locus ID: 29335
• MW: 42.7 kDa
• Gene Summary: This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]