Cbfb (NM_001013191) Rat Tagged ORF Clone

CAT#: RR200748

Cbfb (Myc-DDK-tagged ORF) - Rat core-binding factor, beta subunit (Cbfb), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cbfb
• Synonyms: Pebp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200748 representing NM_001013191
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCGCGTCGTACCGGACCAGAGGAGCAAGTTCGAGAACGAGGAGTTCTTCAGGAAGCTGAGCCGCG
  AGTGTGAGATTAAGTACACGGGCTTCAGGGACCGGCCCCACGAGGAGCGCCAGACACGCTTCCAGAACGC
  TTGCCGCGACGGCCGCTCGGAGATCGCTTTTGTGGCTACAGGAACCAATCTGTCTCTCCAGTTTTTTCCG
  GCCAGCTGGCAGGGAGAGCAACGACAAACACCTAGCCGGGAATATGTCGACTTAGAAAGAGAAGCAGGCA
  AGGTATACTTGAAAGCCCCCATGATTCTGAATGGAGTATGTGTTATATGGAAGGGCTGGATTGATCTCCA
  CAGATTGGATGGTATGGGTTGCCTGGAGTTTGATGAGGAGCGAGCCCAGCAGGAAGATGCATTAGCACAA
  CAGGCCTTTGAAGAGGCTCGAAGAAGAACTCGAGAATTTGAGGATAGAGACAGGTCTCACCGGGAGGAAA
  TGGAGGTGAGAGTTTCACAGCTGCTGGCAGTAACTGGCAAGAAGACAGCAAGACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200748 representing NM_001013191
  Red=Cloning site Green=Tags(s)
  
  MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVATGTNLSLQFFP
  ASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLDGMGCLEFDEERAQQEDALAQ
  QAFEEARRRTREFEDRDRSHREEMEVRVSQLLAVTGKKTARP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013191
• ORF Size: 546 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013191.1, NP_001013209.1
• RefSeq Size: 2927 bp
• RefSeq ORF: 549 bp
• Locus ID: 361391
• Cytogenetics: 19q11
• MW: 21.5 kDa
• Gene Summary: a transcription factor; may play a role in mood stabilization [RGD, Feb 2006]