Myh7 (NM_017240) Rat Tagged ORF Clone

CAT#: RR200853

Myh7 (Myc-DDK-tagged ORF) - Rat myosin, heavy chain 7, cardiac muscle, beta (Myh7), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myh7
• Synonyms: Bmyo; myHC-beta; myHC-slow; Myhcb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200853 representing NM_017240
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATCGAGAGATGGCTGCATTTGGGGCCGGAGCCCCCTTCCTGCGAAAATCTGAGAAGGAGCGGC
  TGGAGGCGCAGACCAGGCCCTTTGACCTCAAGAAAGATGTTTTTGTGCCTGATGACAAAGAAGAGTTTGT
  CAAGGCCAAGATCGTGTCTCGAGAGGGTGGCAAAGTCACCGCTGAGACAGAGAATGGCAAGACGGTGACT
  GTGAAGGAGGACCAGGTGATGCAGCAGAACCCTCCCAAGTTCGACAAGATCGAGGACATGGCCATGCTGA
  CCTTCCTGCACGAGCCGGCTGTGCTCTACAATCTCAAGGAGAGGTACGCTTCCTGGATGATCTACACCTA
  CTCAGGCCTCTTCTGTGTCACCGTCAACCCCTATAAGTGGCTGCCAGTGTACAATGCGCAAGTGGTAGCT
  GCCTACCGGGGCAAGAAGAGGAGCGAGGCTCCACCCCACATCTTCTCCATCTCTGACAACGCCTATCAGT
  ACATGCTGACAGATCGGGAGAACCAGTCCATCCTCATCACCGGAGAATCCGGAGCTGGTAAGACCGTCAA
  CACCAAGAGGGTCATCCAATATTTTGCTGTTATTGCTGCCATTGGGGACCGCAGCAAGAAGGACCAGACC
  CCAGGCAAGGGCACCTTGGAAGATCAAATCATCCAAGCCAACCCCGCTCTGGAGGCCTTTGGCAATGCCA
  AGACAGTTCGGAATGATAACTCCTCCCGATTTGGGAAATTCATTCGAATCCATTTTGGGGCAACAGGAAA
  GTTGGCATCTGCAGATATAGAGACCTACCTTCTGGAAAAATCCAGAGTTATTTTCCAGCTGAAAGCAGAA
  AGAGATTATCACATTTTCTACCAAATCCTGTCTAATAAAAAGCCTGAGCTTCTAGACATGCTGCTGATCA
  CCAACAACCCCTACGATTATGCGTTCTTCTCCCAGGGAGAGACGACTGTGGCCTCAATAGATGACTCTGA
  AGAGCACATGGCCACCGATAGCGCCTTTGATGTGCTGGGCTTCACTCCAGAAGAGAAGAACTCCATTTAC
  AAGCTGACAGGCGCCATCATGCACTTTGGAAACATGAAGTTCAAACAGAAGCAGAGGGAGGAGCAGGCAG
  AGCCAGACGGCACGGAAGAAGCTGACAAGTCAGCCTACCTCATGGGACTGAACTCGGCTGACCTGCTCAA
  GGGGTTGTGCCACCCTCGAGTCAAAGTGGGCAACGAGTATGTCACCAAAGGGCAGAATGTCCAGCAGGTG
  GCATATGCCATCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCATCA
  ACGCAACCCTGGAGACCAAGCAGCCACGCCAGTACTTCATAGGTGTCCTGGACATCGCCGGCTTTGAGAT
  CTTTGATTTCAACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTCAAC
  CACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACGTTTATTGACTTCG
  GCATGGACCTGCAGGCCTGCATCGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTGGAGGAGGA
  GTGCATGTTCCCCAAGGCCACGGACATGACCTTCAAGGCCAAGCTGTACGACAACCACCTGGGCAAGTCC
  AACAACTTCCAGAAGCCTCGCAATATCAAGGGAAAGCAGGAAGCCCACTTCTCTCTGATCCACTATGCTG
  GCACCGTGGACTACAATATCCTGGGCTGGCTACAGAAGAACAAGGACCCTCTCAATGAGACGGTGGTGGG
  GCTGTACCAGAAGTCCTCCCTCAAGCTCCTAAGTAATCTGTTTGCCAACTATGCTGGAGCTGATGCACCT
  GTAGACAAGGGCAAAGGCAAAGCAAAGAAAGGCTCATCCTTTCAGACCGTGTCCGCACTGCACAGGGAAA
  ATCTGAACAAACTTATGACAAACCTGCGCTCCACGCACCCTCACTTTGTACGCTGCATCATCCCCAATGA
  GACGAAGTCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGATGCAACGGAGTGCTGGAG
  GGTATCCGCATCTGTAGGAAGGGCTTCCCCAACCGCATTCTTTATGGGGACTTCCGGCAGAGGTATCGAA
  TCCTGAACCCAGCAGCCATCCCTGAGGGCCAATTCATTGATAGCCGGAAAGGGGCTGAGAAGCTGCTGGG
  CTCCCTGGACATTGACCACAACCAGTACAAGTTTGGCCACACCAAGGTGTTCTTCAAGGCGGGGCTGCTG
  GGGCTGCTGGAGGAGATGCGAGATGAGAGGCTGAGCCGCATCATCACCAGAATCCAGGCCCAGTCCCGAG
  GTGTACTTTCCAGAATGGAGTTTAAGAAGCTGCTGGAGCGCAGAGACTCCCTGCTGATTATCCAGTGGAA
  CATCCGCGCCTTCATGGGGGTCAAGAATTGGCCGTGGATGAAGCTCTACTTCAAGATCAAGCCGCTGCTG
  AAGAGCGCAGAGACAGAGAAGGAGATGGCCAACATGAAGGAGGAGTTCGGGCGAGTCAAAGATGCACTAG
  AGAAGTCTGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAATGACCT
  GCAGCTCCAAGTGCAGGCGGAACAAGACAACCTGGCAGATGCCGAGGAGCGCTGCGACCAGCTGATCAAG
  AACAAGATCCAGCTGGAGGCCAAGGTGAAGGAGATGACCGAGAGGCTGGAGGACGAGGAGGAGATGAACG
  CCGAGCTCACGGCCAAGAAGCGCAAACTGGAAGACGAGTGCTCAGAGCTCAAGAGAGATATCGATGACCT
  GGAGCTGACCCTGGCCAAGGTGGAGAAGGAAAAGCACGCAACAGAGAACAAGGTGAAAAACCTGACAGAG
  GAGATGGCTGGGCTGGACGAGATCATTGTCAAGCTGACCAAGGAGAAGAAAGCTCTACAAGAGGCCCACC
  AGCAAGCCCTAGATGACCTTCAGGCTGAGGAGGACAAGGTCAACACTCTGACCAAGGCCAAGGTCAAGCT
  GGAGCAGCAAGTGGATGATCTGGAGGGATCCCTGGATCAGGACAAGAAGGTGCGCATGGACCTGGAGCGA
  GCAAAGCGGAAGCTGGAGGGTGACCTGAAGCTGACCCAGGAGAGCATCATGGACCTGGAGAACGACAAGC
  AGCAGTTGGATGAGCGACTCAAAAAGAAGGACTTTGAGTTAAATGCACTCAACGCCAGGATTGAGGATGA
  GCAGGCCCTGGGCAGCCAGCTGCAGAAGAAGCTCAAAGAGCTTCAGGCACGCATCGAGGAGCTGGAGGAG
  GAGCTGGAGGCTGAGCGCACAGCCCGGGCCAAGGTGGAGAAGCTGCGCTCAGACCTGTCCCGGGAGCTGG
  AGGAGATCAGTGAGAGGCTAGAGGAAGCCGGTGGGGCCACATCTGTGCAGATAGAGATGAACAAGAAGCG
  CGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTAGAGGAGGCCACGCTGCAGCATGAGGCCACAGCTGCG
  GCCCTGCGCAAGAAACACGCGGACAGCGTGGCCGAGCTGGGCGAGCAGATAGACAATCTACAGCGGGTGA
  AGCAGAAGCTGGAGAAAGAGAAGAGCGAGTTCAAGCTGGAGCTGGATGACGTTACCTCCAACATGGAGCA
  GATCATCAAGGCCAAGGCTAACCTGGAGAAGATGTGCCGGACCCTGGAGGACCAGATGAATGAACACCGG
  AGCAAGGCTGAGGAGACACAGCGTTCTGTCAATGACCTCACCCGCCAGCGGGCCAAGCTGCAGACAGAGA
  ATGGGGAGCTGTCCAGACAGCTGGATGAGAAGGAGGCTCTTATCTCTCAGCTGACCCGAGGCAAGCTCAC
  GTATACCCAGCAGCTGGAGGACCTCAAGAGGCAGCTGGAGGAGGAGGTCAAGGCCAAGAATGCCCTGGCC
  CACGCACTGCAGTCAGCCCGGCATGATTGCGACCTGCTGCGGGAACAGTACGAGGAGGAAACAGAAGCCA
  AGGCTGAGCTGCAGCGTGTCCTGTCCAAGGCCAACTCAGAGGTGGCCCAGTGGAGGACCAAGTATGAGAC
  GGACGCCATACAGAGGACGGAGGAGCTGGAGGAAGCCAAGAAGAAGCTGGCTCAGAGGCTTCAGGATGCT
  GAGGAGGCAGTGGAGGCCGTCAACGCCAAGTGCTCCTCGCTGGAGAAGACCAAGCACAGGCTGCAGAACG
  AGATCGAGGACCTGATGGTGGATGTGGAGCGCTCCAATGCGGCCGCCGCAGCCCTGGACAAGAAGCAGAG
  GAACTTCGACAAGATCCTGGTTGAGTGGAAGCAGAAGTATGAGGAGTCCCAGTCAGAGCTGGAGTCTTCC
  CAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTGAAGAATGCCTATGAGGAGTCTCTGGAGC
  ACCTGGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGGAGGAGATCTCAGACCTGACTGAACAGCTGGG
  CTCAACTGGGAAGAGCATCCACGAGCTGGAGAAGATCCGAAAGCAACTGGAGGCTGAGAAGCTGGAGCTG
  CAGTCAGCCCTGGAAGAGGCTGAGGCCTCCCTGGAGCATGAGGAGGGCAAGATCCTCCGAGCCCAGCTGG
  AGTTCAACCAGATCAAGGCAGAGATCGAAAGGAAGCTGGCAGAGAAGGACGAGGAGATGGAGCAGGCCAA
  GCGCAACCACCTGCGGGTGGTGGACTCCCTGCAGACCTCCCTGGATGCCGAGACGCGCAGCCGCAACGAG
  GCCCTGCGGGTGAAGAAGAAGATGGAGGGCGACCTCAACGAGATGGAGATCCAGCTCAGTCATGCCAACC
  GCATGGCTGCTGAGGCCCAGAAACAAGTGAAGAGCCTCCAGAGTTTGCTGAAGGACACTCAAATCCAGCT
  GGATGACGCAGTCCGTGCCAATGACGACCTGAAGGAGAACATCGCCATCGTGGAGCGGCGCAACAACCTG
  CTGCAGGCGGAGCTGGAGGAGCTGCGGGCCGTGGTGGAGCAGACGGAGCGGTCTCGGAAGCTGGCAGAGC
  AGGAGCTGATCGAGACCAGCGAGCGGGTGCAGCTGCTGCACTCCCAGAACACCAGCCTCATCAACCAGAA
  GAAGAAGATGGATGCAGACCTCTCCCAGCTCCAGACAGAGGTGGAGGAGGCGGTGCAGGAGTGTAGGAAC
  GCAGAGGAGAAGGCCAAGAAGGCCATCACAGATGCCGCCATGATGGCCGAGGAGCTGAAGAAGGAGCAGG
  ACACCAGCGCCCACCTGGAGCGCATGAAGAATAACATGGAGCAGACCATCAAGGACCTGCAGCACCGGCT
  GGACGAGGCAGAGCAGATCGCCCTCAAGGGTGGCAAGAAGCAGCTGCAGAAGCTGGAGGCCCGGGTCCGG
  GAGCTGGAGAATGAGCTGGAGGCTGAGCAGAAGCGCAATGCGGAGTCGGTGAAGGGCATGAGGAAGAGCG
  AGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAGGAAGAACCTACTGCGACTGCAGGACCT
  GGTGGACAAGCTGCAGTTAAAGGTGAAGGCCTACAAGCGCCAGGCTGAGGAGGCGGAGGAACAGGCCAAC
  ACCAACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCAGAGGAGAGGGCGGACATTGCCG
  AGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGTGACATTGGCGCCAAGGGCCTGAATGAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200853 representing NM_017240
  Red=Cloning site Green=Tags(s)
  
  MADREMAAFGAGAPFLRKSEKERLEAQTRPFDLKKDVFVPDDKEEFVKAKIVSREGGKVTAETENGKTVT
  VKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAQVVA
  AYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQT
  PGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAE
  RDYHIFYQILSNKKPELLDMLLITNNPYDYAFFSQGETTVASIDDSEEHMATDSAFDVLGFTPEEKNSIY
  KLTGAIMHFGNMKFKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQV
  AYAIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFN
  HHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKS
  NNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVGLYQKSSLKLLSNLFANYAGADAP
  VDKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLE
  GIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLL
  GLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLL
  KSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIK
  NKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTE
  EMAGLDEIIVKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLDQDKKVRMDLER
  AKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEE
  ELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAA
  ALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHR
  SKAEETQRSVNDLTRQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALA
  HALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDA
  EEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILVEWKQKYEESQSELESS
  QKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSTGKSIHELEKIRKQLEAEKLEL
  QSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNE
  ALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNL
  LQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRN
  AEEKAKKAITDAAMMAEELKKEQDTSAHLERMKNNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVR
  ELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQAN
  TNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKGLNEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017240
• ORF Size: 5805 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017240.1, NM_017240.2, NP_058936.1
• RefSeq Size: 5923
• RefSeq ORF: 5808
• Locus ID: 29557
• MW: 223.1 kDa
• Gene Summary: heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006]