Capn2 (NM_017116) Rat Tagged ORF Clone

CAT#: RR201011

Capn2 (Myc-DDK-tagged ORF) - Rat calpain 2 (Capn2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Capn2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201011 representing NM_017116
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCATCGCGATGAAACTGGCCAAAGACCGCGAGGCGGCCGAGGGGCTGGGGTCTCACGAGAGAG
  CCATCAAGTACCTCAACCAGGACTACGAGACACTGCGGAACGAGTGCCTGGAGGCCGGGGCGCTCTTCCA
  GGATCCTTCCTTCCCCGCCCTGCCGTCGTCCCTGGGCTTCAAGGAGTTGGGGCCCTACTCCAGCAAAACT
  CGGGGCATCGAATGGAAGCGGCCCACGGAGATCTGCGCAGACCCCCAGTTCATTATTGGAGGTGCTACCC
  GCACAGACATCTGCCAAGGAGCCCTTGGGGACTGCTGGCTTCTGGCTGCCATCGCCTCCCTCACCTTGAA
  TGAAGAAATCCTGGCTCGAGTTGTGCCTCTAGACCAGAGCTTCCAGGAAAACTATGCAGGCATCTTCCAT
  TTCCAGTTCTGGCAATATGGCGAATGGGTGGAGGTGGTGGTGGACGACAGACTGCCCACTAAGGACGGGG
  AGCTGCTCTTTGTTCATTCAGCTGAGGGGAGCGAGTTCTGGAGTGCCCTTCTGGAGAAGGCCTATGCCAA
  GATCAATGGGTGCTATGAAGCACTCTCAGGGGGTGCCACCACTGAGGGCTTTGAAGACTTCACAGGAGGC
  ATTGCCGAGTGGTATGAGCTGAGGAAGCCTCCTCCCAATTTGTTCAAGATCATCCAGAAGGCTTTGGAGA
  AAGGTTCTCTGCTTGGCTGCTCTATCGATATCACCAGCGCTGCGGATTCTGAGGCCGTTACGTACCAGAA
  GTTGGTGAAAGGACATGCGTACTCCGTCACTGGAGCCGAGGAGGTTGAAAGCTCAGGAAGCCTGCAGAAA
  TTGATACGTATCAGGAACCCCTGGGGACAAGTGGAGTGGACCGGGAAGTGGAATGACAACTGCCCCAGCT
  GGAACACGGTTGACCCAGAAGTAAGGGCAAATCTAACAGAACGGCAGGAGGACGGAGAATTCTGGATGTC
  CTTCAGTGACTTCCTGAGGCACTACTCTCGTCTAGAAATCTGCAACCTGACCCCGGACACCCTCACCTGT
  GACTCCTACAAGAAGTGGAAACTCACCAAGATGGATGGGAACTGGAGGCGAGGCTCCACTGCAGGGGGCT
  GCAGGAATTACCCAAATACCTTCTGGATGAACCCTCAGTACCTAATCAAGCTGGAGGAAGAAGATGAAGA
  TGATGAGGATGGGGAGAGGGGCTGTACCTTCCTGGTGGGCCTCATCCAGAAGCATCGGCGGCGGCAGAGG
  AAGATGGGTGAGGACATGCACACCATTGGCTTCGGCATCTATGAGGTCCCAGAGGAGCTAACAGGGCAGA
  CCAACATCCACCTCAGCAAAAACTTTTTCCTGACAACCCGAGCCAGGGAGCGGTCAGATACCTTCATCAA
  CCTCCGGGAGGTCCTCAACCGCTTCAAGCTGCCCCCGGGAGAATATGTCCTTGTTCCTTCCACCTTCGAG
  CCCCACAAGAATGGCGATTTCTGCATCCGAGTCTTCTCAGAGAAGAAGGCTGACTACCAAACTGTCGATG
  ATGAAATCGAGGCCAACATTGAAGAGATTGAAGCCAATGAGGAGGACATTGGAGATGGATTCAGAAGGCT
  GTTTGCTCAGCTGGCTGGAGAGGATGCGGAGATCTCTGCCTTTGAGCTGCAGACCATCTTGAGAAGAGTT
  CTAGCCAAGCGCGAAGACATCAAGTCAGATGGCTTCAGCATCGAGACCTGTAAGATCATGGTGGACATGC
  TGGATGAAGATGGGAGCGGCAAGCTGGGGCTGAAGGAGTTCTACATCCTCTGGACGAAGATTCAGAAATA
  CCAAAAAATTTACCGAGAGATCGACGTGGACAGGTCGGGAACCATGAATTCCTATGAGATGCGGAAGGCT
  TTGGAAGAAGCAGGTTTCAAGCTGCCCTGTCAACTTCATCAAGTCATCGTTGCCCGGTTTGCAGATGACG
  AACTCATCATCGACTTTGACAACTTTGTGCGGTGTTTGGTTCGGCTGGAAATACTATTCAAGATATTCAA
  GCAGCTGGACCCTGAGAACACTGGAACGATACAGCTCGACCTTATCTCGTGGCTGAGTTTTTCAGTACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201011 representing NM_017116
  Red=Cloning site Green=Tags(s)
  
  MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGALFQDPSFPALPSSLGFKELGPYSSKT
  RGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLDQSFQENYAGIFH
  FQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGG
  IAEWYELRKPPPNLFKIIQKALEKGSLLGCSIDITSAADSEAVTYQKLVKGHAYSVTGAEEVESSGSLQK
  LIRIRNPWGQVEWTGKWNDNCPSWNTVDPEVRANLTERQEDGEFWMSFSDFLRHYSRLEICNLTPDTLTC
  DSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDDEDGERGCTFLVGLIQKHRRRQR
  KMGEDMHTIGFGIYEVPEELTGQTNIHLSKNFFLTTRARERSDTFINLREVLNRFKLPPGEYVLVPSTFE
  PHKNGDFCIRVFSEKKADYQTVDDEIEANIEEIEANEEDIGDGFRRLFAQLAGEDAEISAFELQTILRRV
  LAKREDIKSDGFSIETCKIMVDMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKA
  LEEAGFKLPCQLHQVIVARFADDELIIDFDNFVRCLVRLEILFKIFKQLDPENTGTIQLDLISWLSFSVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017116
• ORF Size: 2100 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017116.1, NM_017116.2, NP_058812.1
• RefSeq Size: 3556
• RefSeq ORF: 2103
• Locus ID: 29154
• MW: 79.9 kDa
• Gene Summary: putative calcium-binding protease [RGD, Feb 2006]