Prkd3 (NM_001024263) Rat Tagged ORF Clone

CAT#: RR201018

Prkd3 (Myc-DDK-tagged ORF) - Rat protein kinase D3 (Prkd3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkd3
• Synonyms: Prkcn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201018 representing NM_001024263
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCAAATAATTCCCCTCCATCAGCCCAGAAATCTGTGTTCCCTGCAACTGTTTCTGCTGTGCTTC
  CAACCCCTTCTCCGTGTTCAAGTCCCAAGACAGGACTCTCAGCTCGGCTCTCTAATGGCAGCTTCAGTGC
  CGCGTCACTCACAAACTCTAGAGGCTCGGTGCACACCGTTTCATTTCTACTTCAGATTGGCCTCACACGA
  GAGAGTGTTACCATCGAAGCCCAGGAACTGTCTCTCTCCGCTGTCAAAGACCTCGTGTGCTCCATTGTTT
  ATCAGAAGTTTCCAGAGTGTGGATTCTTTGGCATGTATGATAAAATTCTTCTCTTTCGCCACAACATGAA
  CTCAGAAAACATTTTGCAGCTGATTACCTCAGCAGATGAAATACATGAAGGGGATCTTGTAGAAGTTGTC
  CTGTCAGCTTTGGCCACAGTAGAAGACTTCCAGATCCGCCCTCATGCTCTCTATGTCCATTCTTACAAAG
  CACCTACTTTCTGTGATTACTGTGGTGAAATGCTCTGGGGATTGGTACGTCAAGGATTAAAATGTGAAGG
  CTGTGGATTAAATTATCATAAACGATGTGCCTTCAAGATTCCAAATAACTGCAGTGGAGTAAGAAAGAGA
  CGTCTGTCAAATGTGTCTCTGCCAGGACCTGGCCTCTCAGTTCCGAGACCCCTGCAGCCTGAATGTGTGC
  CCCTGCTCAGTGAAGAGTCACATACTCACCAAGAGCCAAGTAAGAGAATCCCGTCCTGGAGTGGTCGTCC
  GATCTGGATGGAAAAGATGGTAATGTGCAGGGTCAAAGTTCCACACACGTTTGCTGTCCACTCTTATGGC
  CGACCCACAATATGTCAGTACTGCAAGCGGCTGCTCAAAGGCCTCTTTCGCCAAGGACTGCAGTGTAAAG
  ATTGCAAATTCAACTGCCACAAACGCTGTGCATCCAAAGTACCACGAGACTGCCTCGGCGAAGTTACTTT
  CAATGGAGAGCCTTCCAGTGTGGGAACTGATACAGACATGCCGATGGATATTGACAGTAATGATGTGAAC
  AGTGATGGCAGTAGGGGCTTGGATGACTCAGAGGAGCCCTCCCCTCCCGAAGACAAAATGTTCTTCCTCG
  ACGCGGCCGATCTGGATGTGGAGCGAGATGAGGAGCCGGTTAAGACAATCAGTCCATCCACAAGCAATAA
  TATCCCACTGATGAGGGTGGTCCAGTCCATCAAGCACACAAAGAGGAAGAGCAGCACAGTGGTGAAGGAA
  GGGTGGATGGTCCACTACACCAGCAGGGACAACCTGAGAAAGAGACATTATTGGAGACTTGACAGCAAAT
  GTCTAACATTGTTTCAGAATGAATCTGGATCAAAATATTATAAGGAAATTCCACTTTCAGAAATTCTCCG
  CATATCTTCGCCACAAGATTTCACCAGCATTTCACAAGGCAGTAACCCACACTGTTTTGAAATCATCACT
  GATACTGTGGTATACTTTGTTGGTGAGAACAATGGGAACAGCACTCATAACCCTGTTCTCGCTGCCACTG
  GAGTGGGACTCGATGTAGCACAAAGCTGGGAAAAAGCGATCCGCCAGGCCCTCATGCCCGTTACTCCTCA
  AGCAAGTGTTTGCACTTCTCCAGGCCAGGGGAAGGACCACAAAGATCTGGCTACCAGTATCTCCGTGTCT
  AACTGTCAGATTCAGGAGAATGTAGACATCAGCAGTGTTTATCAGATCTTTGCCGATGAGGTGCTTGGTT
  CTGGCCAGTTTGGCATTGTTTATGGAGGAAAGCACAGAAAGACAGGAAGGGATGTGGCTATTAAAGTAAT
  CGATAAGATGCGATTCCCCACAAAGCAAGAGAGTCAGCTCCGGAATGAAGTGGCTATTTTACAGAATCTG
  CACCATCCTGGGATTGTAAATCTGGAATGTATGTTTGAAACCCCAGAACGAGTCTTCGTAGTGATGGAAA
  AGCTTCATGGAGATATGTTGGAAATGATTCTGTCCAGTGAAAAAAGTCGACTTCCAGAACGGATTACTAA
  ATTCATGGTCACACAGATACTTGTTGCCCTGAGGAATCTACATTTTAAGAATATCGTGCACTGTGATTTA
  AAGCCCGAAAATGTGCTGCTTGCATCTGCAGAACCTTTTCCTCAGGTGAAGCTATGTGACTTTGGGTTTG
  CCCGCATCATTGGTGAGAAGTCGTTCCGGAGGTCAGTGGTGGGAACTCCAGCCTACTTAGCCCCCGAGGT
  TCTCAGGAGCAAAGGCTACAACCGTTCCCTAGACATGTGGTCGGTGGGAGTCATAGTCTATGTGAGCCTC
  AGTGGCACCTTCCCGTTCAATGAAGATGAAGATATAAACGACCAAATCCAAAATGCTGCATTTATGTACC
  CACCAAATCCATGGCGAGAAATCTCCAGCGAAGCAATTGATTTGATAAACAACTTGCTTCAAGTGAAGAT
  GAGAAAACGTTACAGCGTTGACAAGTCCCTCAGTCATCCCTGGCTACAGGACTATCAGACTTGGCTTGAC
  CTCAGAGAATTCGAAACTCGCATTGGAGAACGTTACATCACACATGAAAGTGATGATGCTCGCTGGGAAA
  TACATGCATACACGCACAATCTCGAATACCCAAAACATTTCATTATGGCCCCCAACCCGGATGACATGGA
  GGAAGGTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201018 representing NM_001024263
  Red=Cloning site Green=Tags(s)
  
  MSANNSPPSAQKSVFPATVSAVLPTPSPCSSPKTGLSARLSNGSFSAASLTNSRGSVHTVSFLLQIGLTR
  ESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHNMNSENILQLITSADEIHEGDLVEVV
  LSALATVEDFQIRPHALYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKR
  RLSNVSLPGPGLSVPRPLQPECVPLLSEESHTHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYG
  RPTICQYCKRLLKGLFRQGLQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPSSVGTDTDMPMDIDSNDVN
  SDGSRGLDDSEEPSPPEDKMFFLDAADLDVERDEEPVKTISPSTSNNIPLMRVVQSIKHTKRKSSTVVKE
  GWMVHYTSRDNLRKRHYWRLDSKCLTLFQNESGSKYYKEIPLSEILRISSPQDFTSISQGSNPHCFEIIT
  DTVVYFVGENNGNSTHNPVLAATGVGLDVAQSWEKAIRQALMPVTPQASVCTSPGQGKDHKDLATSISVS
  NCQIQENVDISSVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEVAILQNL
  HHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFMVTQILVALRNLHFKNIVHCDL
  KPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGVIVYVSL
  SGTFPFNEDEDINDQIQNAAFMYPPNPWREISSEAIDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLD
  LREFETRIGERYITHESDDARWEIHAYTHNLEYPKHFIMAPNPDDMEEGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024263
• ORF Size: 2670 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024263.2, NP_001019434.2
• RefSeq Size: 5769 bp
• RefSeq ORF: 2673 bp
• Locus ID: 313834
• Cytogenetics: 6q11
• MW: 100.2 kDa