Kng1 (NM_001009628) Rat Tagged ORF Clone

CAT#: RR201106

Kng1l1 (Myc-DDK-tagged ORF) - Rat kininogen 1-like 1 (Kng1l1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kng1
• Synonyms: kininogen; Kng1l1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201106 representing NM_001009628
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTAATTACTATCCTGCTCCTCTGCTCCAGGCTCCTGCCAAGTTTAGCGCAGGAAGAAGGTGCCC
  AGGAAATGGACTGCAATGATGAGACTGTATTTCAGGCTGTGGATACTGCTCTGAAGAAATATAATGCTGA
  GTTAGAAAGTGGCAACCAGTTTCTGTTGTACCGAGTGACTGAGGGCACTAAGAAGGATGGCGCTGAAACA
  TTGTATTCCTTCAAGTATCAAATCAAGGAGGGCAACTGCTCTGTTCAGAGTGGCCTCACCTGGCAGGACT
  GTGACTTCAAGGACGCTGAGGAAGCTGCTACTGGCGAATGCACAACAACTTTGGGGAAGAAAGAAAATAA
  ATTCTCCGTAGCCACCCAGATCTGCAATATTACTCCAGGTAAGGGTCCTAAGAAGACAGAGGAGGACCTC
  TGTGTCGGGTGTTTCCAACCCATACCGATGGATAGCTCAGACCTGAAGCCTGTTCTGAAACACGCTGTGG
  AGCATTTCAACAACAACACGAAGCACACCCACCTCTTTGCTCTCACAGAAGTAAAGAGTGCCCACTCACA
  GGTGGTGGCTGGCATGAATTATAAAATTATCTACTCCATTGTGCAAACAAATTGTTCAAAGGAGGATTTT
  CCTTTCCTCCGTGAAGACTGTGTACCCCTTCCCTATGGCGATCATGGTGAGTGTAGGGGTCATACCTACG
  TGGATATTCATAACACAATTGCTGGCTTCTCACAGAGCTGTGACCTTTATCCAGGAGACGATTTGTTTTC
  ACTACTTCCCAAGAAATGCTTTGGCTGCCCCAAGAACATACCTGTAGACAGCCCGGAGCTCAAGGAGGCA
  CTTGGTCATTCAATTGCACAGCTTAATGCACAGCATAATCATCTTTTCTATTTCAAGATTGACACCGTGA
  AAAAGGCAACATCACAGGTGGTTGCTGGAACAAAATATGTGATTGAGTTCATAGCCAGAGAAACCAATTG
  TTCCAAACAAACTAACACAGAACTGACAGCGGACTGTGAGACCAAACACCTCGGTCAAAGCCTCAACTGC
  AATGCTAACGTGTACATGAGACCTTGGGAGAACAAAGTCGTCCCGACTGTCAGATGCCAAGCGCTAGACA
  TGATGATTTCTAGGCCTCCAGGATTTTCACCTTTCCGGCTGGTGCAAGTACAAGAAACTAAAGAAGGAAC
  AACTAGGCTCCTAAACTCATGTGAGTACAAGGGCAGACTCTCAAAGGCAGGGGCAGGCCCAGCGCCTGAC
  CATCAGGCAGAAGCTTCAACCGTGACACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201106 representing NM_001009628
  Red=Cloning site Green=Tags(s)
  
  MKLITILLLCSRLLPSLAQEEGAQEMDCNDETVFQAVDTALKKYNAELESGNQFLLYRVTEGTKKDGAET
  LYSFKYQIKEGNCSVQSGLTWQDCDFKDAEEAATGECTTTLGKKENKFSVATQICNITPGKGPKKTEEDL
  CVGCFQPIPMDSSDLKPVLKHAVEHFNNNTKHTHLFALTEVKSAHSQVVAGMNYKIIYSIVQTNCSKEDF
  PFLREDCVPLPYGDHGECRGHTYVDIHNTIAGFSQSCDLYPGDDLFSLLPKKCFGCPKNIPVDSPELKEA
  LGHSIAQLNAQHNHLFYFKIDTVKKATSQVVAGTKYVIEFIARETNCSKQTNTELTADCETKHLGQSLNC
  NANVYMRPWENKVVPTVRCQALDMMISRPPGFSPFRLVQVQETKEGTTRLLNSCEYKGRLSKAGAGPAPD
  HQAEASTVTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009628
• ORF Size: 1290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009628.1, NP_001009628.1
• RefSeq Size: 1519 bp
• RefSeq ORF: 1293 bp
• Locus ID: 288001
• Cytogenetics: 11q23
• MW: 47.7 kDa
• Gene Summary: Kininogens are plasma glycoproteins with a number of functions: (1) as precursor of the active peptide bradykinin they effect smooth muscle contraction, induction of hypotension and increase of vascular permeability. (2) They play a role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII. (3) They are inhibitor of thiol proteases. [UniProtKB/Swiss-Prot Function]