Atg9a (NM_001014218) Rat Tagged ORF Clone

CAT#: RR201285

Atg9a (Myc-DDK-tagged ORF) - Rat ATG9 autophagy related 9 homolog A (S. cerevisiae) (Atg9a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atg9a
• Synonyms: RGD1310450
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201285 representing NM_001014218
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACAGTTTGACACTGAATACCAGCGCCTAGAGGCCTCTTATAGCGATTCACCCCCAGGGGAAGAGG
  ACCTGTTGGTGCATGTGGCTGAGGGGAGCAAATCACCTTGGCACCACATTGAAAACCTTGACCTCTTCTT
  CTCTCGAGTTTATAATCTACATCAGAAGAATGGGTTCACATGTATGCTCATTGGAGAGATTTTTGAACTC
  ATGCAGTTCCTCTTTGTGGTTGCCTTCACCACCTTCCTGGTTAGCTGTGTGGACTATGACATCCTATTTG
  CCAACAAGATGGTGAACCACAGTCTGCATCCTACCGAGCCTGTCAAGGTCACTCTGCCAGATGCCTTTTT
  GCCTGCCCAAGTTTGCAGTGCCAGGATTCAGGAAAACGGCTCCCTCATCACCATCCTGGTCATCGCTGGT
  GTCTTCTGGATCCACCGGCTTATCAAGTTTATCTATAACATTTGCTGCTATTGGGAGATCCACTCCTTCT
  ACCTACATGCTCTGCGGATCCCAATGTCTGCCCTTCCGTATTGCACGTGGCAGGAAGTGCAGGCCCGGAT
  TGTGCAGACCCAGAAAGAGCATCAGATCTGCATCCACAAGCGTGAGCTGACAGAGTTGGACATCTACCAT
  CGCATCCTCCGTTTCCAGAACTACATGGTGGCACTGGTGAACAAATCCCTCTTGCCTCTGCGCTTCCGTC
  TGCCCGGCCTCGGAGAGGTTGTCTTCTTCACCCGTGGCCTCAAGTACAACTTTGAGCTCATCCTCTTCTG
  GGGACCCGGCTCTCTGTTTCTCAATGAATGGAGCCTCAAGGCCGAGTACAAACGTGGAGGGCAACGGCTA
  GAGCTGGCCCAGCGTCTCAGCAACCGCATCCTGTGGATTGGCATCGCCAACTTCCTGCTCTGTCCCCTCA
  TCCTCATCTGGCAGATCCTCTATGCCTTCTTCAGCTATGCCGAGGTGCTGAAGAGAGAGCCGGGAGCCCT
  GGGAGCGCGTTGCTGGTCACTCTATGGCCGTTGCTACCTCCGCCACTTCAATGAGCTAGAACACGAGCTG
  CAGTCCCGCCTCAACCGAGGATACAAGCCCGCCTCCAAGTACATGAATTGCTTCTTGTCACCGCTACTGA
  CTCTGCTGGCCAAGAATGGTGCCTTCTTCGCGGGCTCTATCCTGGCCGTGCTTATTGCTCTCACGATCTA
  CGATGAAGATGTGTTGGCTGTGGAGCATGTCCTCACCACTGTCACCCTACTGGGAGTCACGGTGACTGTG
  TGCAGGTCTTTCATCCCAGACCAGCACATGGTGTTCTGCCCTGAGCAGCTGCTCCGAGTGATCCTTGCCC
  ACATCCACTACATGCCTGACCACTGGCAGGGTAATGCCCACCGCTCGCAGACCCGGGACGAGTTTGCCCA
  GCTCTTCCAGTACAAGGCAGTGTTCATCTTGGAGGAGTTGCTGAGTCCCATTGTTACACCCCTCATTCTC
  ATCTTCTGCCTACGCCCACGGGCCCTGGAGATCATAGACTTCTTCCGAAACTTTACTGTGGAGGTCGTCG
  GTGTGGGGGACACCTGCTCCTTTGCGCAGATGGACGTTCGCCAGCACGGTCATCCTCAGTGGCTGTCTGG
  AGGGCAGACTGAGGCCTCAGTGTACCAGCAAGCCGAGGATGGGAAGACCGAGTTGTCACTCATGCACTTT
  GCCATCACCAATCCTGGCTGGCAGCCCCCTCGTGAGAGCACAGCCTTCCTGGGCTTCCTCAAGGAGCAGG
  TGCAGCGAGATGGAGCGGCTGCTGGCCTTGCCCAGGGTGGTCTGCTCCCAGAGAATGCCCTTTTCACATC
  CATCCAGTCCTTACAGTCTGAGTCTGAGCCACTGAGCCTCATTGCAAATGTGGTAGCAGGCTCGTCCTGC
  CGAGGACCCCCACTGTCCAGAGACCTGCAGGGTTCCAGGCACAGGGCTGATGTTGCTTCTGCCCTTAGAT
  CCTTCTCCCCTCTACAGCCTGGACAGGCCCCTCAAGGCCGTGTTCCCAGTACCATGACAGGCTCTGGAGT
  GGACGCCAGGACAGCCAGCTCCGGGAGCAGCGTGTGGGAAGGACAGCTGCAAAGCCTGGTGCTGTCTGAA
  TATGCGTCCACTGAGATGAGCCTTCATGCCCTCTATATGCACCAGCTCCACAAGCAGCAGACCCAGGCTG
  AGCCCGAGCGGCATGTGTGGCACCGCCGGGAGAGTGATGAGAGTGGAGAGAGTGCCCCTGAAGAGGGGGG
  AGAGGGTGCCCGGGCCCCCCAACCCATCCCACGCTCTGCCAGCTATCCCTGTGCTACTCCCCGGCCTGGA
  GCACCTGAGACCACCGCCCTGCATGGGGGCTTCCAGAGGCGCTACGGGGGCATCACAGATCCTGGCACAG
  TGCCCCGTGCTCCCTCTCACTTCTCCAGGCTGCCCTTGGGAGGATGGGCAGAAGATGGGCAGCCAGCATC
  AAGACACCCAGAGCCGGTGCCAGAGGAGGGCTCAGAGGATGAACTGCCCCCTCAAGTGCACAAGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201285 representing NM_001014218
  Red=Cloning site Green=Tags(s)
  
  MAQFDTEYQRLEASYSDSPPGEEDLLVHVAEGSKSPWHHIENLDLFFSRVYNLHQKNGFTCMLIGEIFEL
  MQFLFVVAFTTFLVSCVDYDILFANKMVNHSLHPTEPVKVTLPDAFLPAQVCSARIQENGSLITILVIAG
  VFWIHRLIKFIYNICCYWEIHSFYLHALRIPMSALPYCTWQEVQARIVQTQKEHQICIHKRELTELDIYH
  RILRFQNYMVALVNKSLLPLRFRLPGLGEVVFFTRGLKYNFELILFWGPGSLFLNEWSLKAEYKRGGQRL
  ELAQRLSNRILWIGIANFLLCPLILIWQILYAFFSYAEVLKREPGALGARCWSLYGRCYLRHFNELEHEL
  QSRLNRGYKPASKYMNCFLSPLLTLLAKNGAFFAGSILAVLIALTIYDEDVLAVEHVLTTVTLLGVTVTV
  CRSFIPDQHMVFCPEQLLRVILAHIHYMPDHWQGNAHRSQTRDEFAQLFQYKAVFILEELLSPIVTPLIL
  IFCLRPRALEIIDFFRNFTVEVVGVGDTCSFAQMDVRQHGHPQWLSGGQTEASVYQQAEDGKTELSLMHF
  AITNPGWQPPRESTAFLGFLKEQVQRDGAAAGLAQGGLLPENALFTSIQSLQSESEPLSLIANVVAGSSC
  RGPPLSRDLQGSRHRADVASALRSFSPLQPGQAPQGRVPSTMTGSGVDARTASSGSSVWEGQLQSLVLSE
  YASTEMSLHALYMHQLHKQQTQAEPERHVWHRRESDESGESAPEEGGEGARAPQPIPRSASYPCATPRPG
  APETTALHGGFQRRYGGITDPGTVPRAPSHFSRLPLGGWAEDGQPASRHPEPVPEEGSEDELPPQVHKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014218
• ORF Size: 2517 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014218.1, NP_001014240.1
• RefSeq Size: 4134 bp
• RefSeq ORF: 2520 bp
• Locus ID: 363254
• Cytogenetics: 9q33
• MW: 94.5 kDa