Bace1 (NM_019204) Rat Tagged ORF Clone

CAT#: RR201423

Bace1 (Myc-DDK-tagged ORF) - Rat beta-site APP cleaving enzyme 1 (Bace1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bace1
• Synonyms: Bace
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201423 representing NM_019204
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGGCGCTGCGCTGGCTCCTGCTATGGGTGGGCTCGGGAATGCTGCCTGCCCAGGGAACCCATC
  TCGGTATCCGACTGCCCCTTCGCAGCGGCCTGGCAGGGCCACCCCTGGGCCTGAGGCTGCCCCGGGAGAC
  GGACGAGGAACCTGAGGAGCCTGGCCGGAGAGGCAGCTTTGTGGAGATGGTGGACAACCTGAGGGGAAAG
  TCCGGCCAGGGCTACTATGTGGAGATGACCGTGGGCAGCCCCCCACAGACGCTCAACATCCTGGTGGACA
  CGGGCAGTAGTAATTTTGCAGTGGGGGCTGCCCCACACCCTTTCCTGCATCGATACTACCAAAGGCAGCT
  GTCCAGTACATACCGAGACCTCCGAAAGTCTGTGTATGTGCCCTACACCCAGGGCAAGTGGGAGGGGGAA
  CTGGGCACTGACCTGGTGAGCATCCCTCATGGCCCCAACGTCACTGTGCGTGCCAACATTGCTGCCATCA
  CTGAATCGGACAAGTTCTTCATCAATGGTTCCAACTGGGAGGGCATCCTAGGGCTGGCCTATGCTGAGAT
  TGCCAGGCCTGACGACTCCTTGGAGCCCTTTTTTGACTCCCTGGTGAAGCAGACCCACATTCCGAACATC
  TTTTCCCTGCAGCTCTGTGGCGCTGGCTTCCCCCTCAACCAGACTGAGGCACTGGCCTCGGTGGGAGGGA
  GCATGATCATTGGTGGTATCGACCATTCCCTATACACTGGCAGTCTCTGGTACACACCCATCCGGCGGGA
  GTGGTATTATGAAGTGATCATTGTACGTGTAGAAATCAATGGTCAAGATCTGAAAATGGACTGCAAGGAG
  TACAACTATGACAAGAGCATCGTGGACAGTGGCACCACCAACCTTCGTTTGCCCAAGAAAGTATTTGAAG
  CTGCAGTCAAGTCCATCAAGGCAGCCTCCTCGACGGAGAAGTTCCCGGATGGCTTTTGGCTAGGGGAGCA
  GCTGGTGTGCTGGCAAGCAGGCACGACCCCTTGGAACATTTTCCCAGTCATTTCACTTTACCTCATGGGT
  GAAGTCACCAATCAGTCCTTCCGCATCACCATCCTTCCTCAGCAATACCTACGGCCAGTGGAAGATGTGG
  CCACGTCCCAAGACGACTGTTACAAGTTCGCCGTCTCACAGTCATCCACAGGCACCGTTATGGGAGCGGT
  CATCATGGAAGGCTTCTATGTGGTCTTTGATCGAGCCCGAAAGCGAATTGGCTTTGCTGTCAGCGCTTGC
  CATGTGCACGATGAGTTCAGGACGGCGGCAGTGGAAGGTCCGTTTGTCACGGCAGACATGGAAGACTGTG
  GCTACAACATTCCACAGACAGATGAGTCAACACTTATGACCATAGCCTATGTCATGGCTGCCATCTGCGC
  CCTCTTCATGTTGCCACTCTGCCTCATGGTATGTCAGTGGCGCTGCCTACGCTGCCTGCGCCATCAGCAT
  GATGACTTTGCTGATGACATCTCCCTGCTGAAAGATGACTTTGCTGATGACATCTCCCTGCTGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201423 representing NM_019204
  Red=Cloning site Green=Tags(s)
  
  MAPALRWLLLWVGSGMLPAQGTHLGIRLPLRSGLAGPPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGK
  SGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKSVYVPYTQGKWEGE
  LGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHIPNI
  FSLQLCGAGFPLNQTEALASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKE
  YNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMG
  EVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSAC
  HVHDEFRTAAVEGPFVTADMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRHQH
  DDFADDISLLKDDFADDISLLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019204
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq Size: 2158
• RefSeq ORF: 1506
• Locus ID: 29392
• MW: 55.8 kDa
• Gene Summary: catalyzes the cleavage of the beta site of amyloid precursor protein; may play a role in generation of amyloid beta peptide [RGD, Feb 2006]