Akr1c2 (NM_001013057) Rat Tagged ORF Clone

CAT#: RR201425

Akr1c2 (Myc-DDK-tagged ORF) - Rat aldo-keto reductase family 1, member C21 (Akr1c21), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Akr1c2
• Synonyms: Akr1c21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201425 representing NM_001013057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCAAATGTCATTGTGTGAAACTGAACGATGGTCATTTCATTCCGGTGCTGGGTTTTGGAACTG
  CTATGCCTTCAGAGTTACCCAAGAGTAAAGCTAAGGAGGTCACCAAAATAGCTATAGATGCTGGTTTCCA
  TCATTTTGATTCTGCTTTTGTCTACAATACCGAAGATCATGTAGGAGAGGCCATCCGAGAGAAGATTGCC
  AATGGCACTACAAGGAGAGAAGATATATTTTACACATCAAAGCTTTGGTGTACGAGCCTTCACCCAGAAC
  TGGTGAGATCTTCCTTGGAATGTTCACTGAAGAAACTTCAGTTGGACTATGTGGACCTGTATCTCATACA
  TTTTCCGATGGCCTTGAAACCAGGAGATGAAAATTTTCCAGTAGATGAACATGGAAAGTTACTATTTGAC
  ACAGTAGATCTCTGTGCTACCTGGGAGGCCATGGAGAAGTGTAAGGATGCAGGACTGGCCAAGTCCATCG
  GGGTGTCCAACTTTAACCGCAGGCAGCTGGAGAAGATCCTGAACAAGCCGGGGCTCAAATACAAGCCTGT
  GTGCAACCAGGTAGAATGCCATCTTTATCTCAACCAAATGAAACTGCTGGATTTCTGCAAGACAAATGGC
  ATTATACTGGTTGCCTATGGAGTTCTGGGAACACAACGATATAACGGATGGGTAGACCAGAACTCCCCTG
  TTCTCTTGAATGAACCAGTTCTTAGTTCCATGGCAAAAAAATACAATCAAACTCCAGCCTTGATTGCCCT
  TCGCCATCAGTTGCAGCGTGGGATTGTGGTACTTAACACCAGTCTCAAAGAAGAGCGGATCAAAGAGAAC
  ATGAAGCTGAGTCCAGAAGATATGAAAGTCCTTGATGACCTGAACAGAAATTTGCGGTACATAGCTGGTG
  GAATATTTGAAGGCCACCCTAATTTTCCGTTTTTGGATGAATACGAATATTTGAAGGCCACCCTAATTTT
  CCGTTTTTGGATGAATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201425 representing NM_001013057
  Red=Cloning site Green=Tags(s)
  
  MNSKCHCVKLNDGHFIPVLGFGTAMPSELPKSKAKEVTKIAIDAGFHHFDSAFVYNTEDHVGEAIREKIA
  NGTTRREDIFYTSKLWCTSLHPELVRSSLECSLKKLQLDYVDLYLIHFPMALKPGDENFPVDEHGKLLFD
  TVDLCATWEAMEKCKDAGLAKSIGVSNFNRRQLEKILNKPGLKYKPVCNQVECHLYLNQMKLLDFCKTNG
  IILVAYGVLGTQRYNGWVDQNSPVLLNEPVLSSMAKKYNQTPALIALRHQLQRGIVVLNTSLKEERIKEN
  MKLSPEDMKVLDDLNRNLRYIAGGIFEGHPNFPFLDEYEYLKATLIFRFWMN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013057
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1398 bp
• RefSeq ORF: 957 bp
• Locus ID: 291283
• Cytogenetics: 17q12.2
• MW: 36.1 kDa