Grpr (NM_012706) Rat Tagged ORF Clone

CAT#: RR201544

Grpr (Myc-DDK-tagged ORF) - Rat gastrin releasing peptide receptor (Grpr), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grpr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201544 representing NM_012706
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCAAATAATTGTTCCCACCTGAACTTGGAAGTGGATCCTTTCCTGTCCTGCAACAACACCTTCA
  ATCAAACTCTGAGTCCCCCCAAGATGGACAACTGGTTTCACCCGGGAATCATCTATGTCATTCCTGCAGT
  TTATGGGCTTATCATCGTGATAGGCCTCATTGGCAACATCACCCTGATCAAGATCTTCTGTACGGTCAAG
  TCCATGCGAAACGTGCCAAACCTGTTCATCTCTAGCCTGGCATTGGGAGACCTACTGCTGCTGGTTACAT
  GCGCCCCTGTGGATGCCAGCAAGTACCTGGCTGACAGGTGGCTATTTGGCAGGATTGGCTGCAAACTGAT
  CCCCTTTATACAACTTACTTCAGTGGGGGTGTCTGTCTTCACACTTACGGCACTGTCAGCTGACAGGTAC
  AAAGCCATCGTCAGGCCAATGGATATCCAGGCATCCCATGCCCTGATGAAGATCTGTCTCAAAGCTGCCT
  TAATCTGGATCGTCTCTATGCTGTTGGCCATCCCAGAGGCTGTATTTTCGGACCTTCACCCCTTTCATGT
  GAAAGATACCAACCAAACCTTCATTAGTTGTGCCCCCTACCCACACTCCAATGAGCTGCACCCTAAAATC
  CATTCCATGGCTTCCTTTCTGGTCTTTTACATTATCCCACTGTCGATCATCTCTGTCTACTACTACTTCA
  TTGCCCGAAATCTGATTCAGAGTGCCTACAATCTTCCTGTGGAAGGAAATATACATGTCAAGAAGCAGAT
  CGAATCCCGGAAGCGGCTTGCCAAGACAGTACTGGTGTTTGTGGGCCTCTTCGCCTTCTGCTGGCTCCCC
  AATCATGTCATCTACCTGTACCGTTCCTACCACTACTCTGAGGTGGACACCTCCATGCTCCACTTTATCA
  CCAGCATCTGTGCCCGCCTCCTGGCCTTCACCAACTCCTGTGTGAACCCCTTTGCCCTGTATCTGCTGAG
  CAAGAGCTTCAGGAAGCAGTTCAACACTCAACTTCTCTGCTGCCAGCCCAGCCTGTTGAACAGGTCTCAC
  AGCACAGGCAGAAGTACCACCTGCATGACCTCCTTCAAGAGCACCAACCCCTCAGCTACCTTCAGCCTCA
  TCAACGGAAATATCTGCCATGAGGGGTATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201544 representing NM_012706
  Red=Cloning site Green=Tags(s)
  
  MDPNNCSHLNLEVDPFLSCNNTFNQTLSPPKMDNWFHPGIIYVIPAVYGLIIVIGLIGNITLIKIFCTVK
  SMRNVPNLFISSLALGDLLLLVTCAPVDASKYLADRWLFGRIGCKLIPFIQLTSVGVSVFTLTALSADRY
  KAIVRPMDIQASHALMKICLKAALIWIVSMLLAIPEAVFSDLHPFHVKDTNQTFISCAPYPHSNELHPKI
  HSMASFLVFYIIPLSIISVYYYFIARNLIQSAYNLPVEGNIHVKKQIESRKRLAKTVLVFVGLFAFCWLP
  NHVIYLYRSYHYSEVDTSMLHFITSICARLLAFTNSCVNPFALYLLSKSFRKQFNTQLLCCQPSLLNRSH
  STGRSTTCMTSFKSTNPSATFSLINGNICHEGYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012706
• ORF Size: 1152 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012706.1, NM_012706.2, NP_036838.1
• RefSeq Size: 1308
• RefSeq ORF: 1155
• Locus ID: 24938
• MW: 43.7 kDa
• Gene Summary: G-protein coupled receptor for the mammalian bombesis peptide, may be involved in the development and/or maintenace of specific hindbrain segments [RGD, Feb 2006]