Rad50 (NM_022246) Rat Tagged ORF Clone

CAT#: RR201717

Rad50 (Myc-DDK-tagged ORF) - Rat RAD50 homolog (S. cerevisiae) (Rad50), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rad50
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201717 representing NM_022246
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGGATCGAAAAGATGAGCACTCTGGGCGTGCGAAGTTTTGGGATAGAGGATAAAGATAAGCAAA
  TTATCTCTTTCTTCAGCCCCCTCACAATTTTGGTTGGACCCAATGGGGCGGGGAAGACGACCATCATTGA
  ATGTCTAAAGTATATTTGTACTGGAGATTTCCCTCCTGGAACCAAAGGAAATACATTTGTTCATGATCCC
  AAGGTTGCTCAAGAAACAGATGTGCGTGCCCAAATTCGCCTGCAGTTTCGTGATGTCAATGGAGAGATGG
  TACTTGTGCAGAGGTCCATGCTTTGCAGTCAGAAAAGTAAAAAAACCGAATTTAAAACCCTGGAAGGAGT
  CATTACTAGAATAAAGCACGGTGAAAAAGTCAGTCTCAGCTCCAAATGTGCAGAAATCGACCGAGAAATG
  ATAAGTTGTCTTGGGGTTTCCAAGTCTGTGCTAAACAATGTTATTTTCTGCCACCAAGAAGACTCAAATT
  GGCCTCTAAGTGAAGGAAAGGCTCTGAAGCAGAAATTTGATGAGATTTTTTCAGCAACAAGGTACATTAA
  AGCCCTAGATACGCTTCGACAGGTACGACAGACACAAGGTCAGAAAGTAAAAGAATGTCAAACAGAATTG
  AAATATCTGAGGCAAAATAAGGAGAAAGCTTGTGAGATCCGAGATCAGATCACTAGTAAGGAAGCCCAGT
  TAGCATCTTCACGGGAAATTGTCAAAGCCTATGAGAATGAGCTTGAGCCATTGAAGAATCGCCTGAAAGA
  GATTGAACATAACCTCTCTAAAATAATGAGACTTGACAATGAAATTAAAGCCTTGGATAGCAGAAAGAAG
  CAAATGGAAAAAGATAACAGTGAATTAGAACAGAAGATGGAAAAGGTTTTTCAAGGGACTGATGAGCAGC
  TAAATGACTTGTATCACAATCACCAGAGAACTGTAAGGGAGAAAGAAAGGCGCTTGGTAGACTGTCAGCG
  TGAACTGGAGAAGCTGAGTAAAGAAGCTCGGCTCCTCAACCAGGAAAGAGCAGAGCTGCTTGTGGAGCAG
  GGTCGTCTACAACTACAGGCAGATCGACATCAAGAGCATATCCGAGCCAGAGACTCACTGATTCAGTCTT
  TGGCAGCACATCTTGAATTGGATGGTTTTGAGCGTGGACCATTCAGTGAAAGACAGATTAAAAACTTTCA
  TGAACTTGTGAGAGAGAGACAGGAGAGAGAAGCTAAAACTGCCAGCCAGCTCTTGAGCGACCTTACAGAC
  AAAGAAGCGCTGAAGCAGAGACAGATGGATGAGATGAGGGACAAGAAGAGCGGGCTGGGGAGGATGATTG
  AGCTGAAGACCGAGATCCTGACGAAGAAGCAGACTGAGCTGAGGAACGTGAGGAATGAGCTGCAGCAGCT
  GGAGGGCTCCTCGGACAGGATTCTGGAGCTGGACCAGGAGCTCACAAAAGCGGAACGTGAACTAAGCAAG
  GCTGAGAAAAATAGCAGCATAGAAACCCTAAAAGCAGAAATACTAAACCTCCAAAGTGAGAAAGCGGACC
  TGGACAGGAACCTGCGGAAACTGGATCAGGAGATGGAGCAGTTAAACCATCATACAACAACCCGCACACA
  GATGGAGATGCTTACCAAAGACAAAACTGACAAAGATGAACAGATCAGAAAAATAAAGTCCAGGCACAGT
  GATGAACTAACTTCACTGTTGGGATATTTTCCTAACAAAAAACAGCTTGAAGACTGGCTTCATTCTAAAT
  CCAAAGAGATTAATCAGACCAGGGACAGACTTGCCAAACTGAACAAAGAACTAGCTTCAGCCGAACAAAA
  TAAAAATCATATAAATAATGAGCTAAAGAAAAAGGAAGAGCAGCTGTCTAGTTATGAAGATAAACTGTTT
  GATGTTTGTGGTAGCCAAGATTTTGAAAGTGACTTAGACAGACTTAAAGAAGATATTGAAAAATCCTCAA
  AGCAGCGAGCCATGCTGGCTGGAGCCACAGCAGTTTACTCCCAGTTCATCACTCAGCTGACAGATGAAAA
  CCAGTCCTGTTGCCCAGGCTGTCAGAGAGTATTTCAGACGGAAGCTGAATTACAGGAAGTCATCAGTGAC
  TTGCAGTCCAAGCTGAGGCTTGCTCCAGATAAACTCAAGTCAACAGAATCAGAACTAAAAAAAAAAGAGC
  GGCGCCGTGATGAAATGCTGGGGCTTGTGCCCATGAGGCAAAGCATAATTGATTTGAAGGAAAAGGAAAT
  ACCAGAATTAAGAAACAGACTGCAGAGTGTCAATAGAGACATACAGCGCCTAAAGAATGACATAGAGGAG
  CAGGAGACACTCTTGGGTACAGTGATGCCCGAAGAGGAAAGTGCTAAAGTGTGCCTGACAGACGTCACGA
  TCATGGAGAGGTTCCAGATGGAGCTAAAAGACGTTGAAAGGAAAATTGCACAGCAGGCAGCTAAGCTGCA
  GGGGGTAGACTTGGATCGGACTGTCCAGCAGGTTAACCAGGAAAAACAAGAAAAACAACACAAACTGGAT
  ACAGTTTCCAGTAAGATTGAATTGAACCGTAAGCTTATACAGGACCAGCAGGAACAAATTCAGCACCTGA
  AAAGTAAAACAAATGAGCTGAAATCAGAGAAACTGCAGATAGCCACCAATTTGCAACGGCGTCAGCAAAT
  GGAGGAGCAGACTGTGGAATTATCCACTGAAGTTCAGTCTTTGAACAGAGAAATAAAGGATGCTAAAGAG
  CAAATAAACCCTTTGGAGATAGCACTGGAAAAGTTGCAGCAGGAAAAAGAAGAATTGATCCACAGAAAAA
  ATACAAGTAACAAAATGGCTCAGGATAAGATCAATGATATCAAAGAGAAAGTTAAAAATATTCATGGTTA
  CATGAAAGACATAGAGAATTATATTCAAGATGGAAAAGATGACTATAAGAAGCAAAAAGAAACTGAACTT
  AATGAAGTTGTCATTCAACTAAATGAATGTGACAAACACAAAGAAAAGATAAATAAAGAAATGGGAACCA
  TGAGGCAAGATATTGACACGAAGAAGATACAGGAAAGGTGGTTACAGGATAACCTTACACTGAGAAAAAG
  AAGAGAGGAACTAAAAGAAGTTGAAGAGGAACGAAAGCAACATTTGAAGGAGATGGGACAGATGCAAGTT
  TTACAGATGAAAAATGAGCACCAGAAGTTGGAAGAGAACATAGACACGATAAAGAGAAATCACAGTTTGG
  CATTGGGGCGACAGAAAGGCTATGAAGAAGAAATTCTTCACTTTAAGAAGGAGCTCCGAGAACCTCAGTT
  TCGGGATGCAGAGGAGAAGTACAGAGAAATGATGATTGTCATGAGAACCACAGAGCTGGTGAATAAGGAC
  CTGGACATCTACTACAAAACTCTTGATCACGCAATAATGAAATTTCACAGTATGAAAATGGAAGAAATCA
  ACAAAATTATTCGTGATCTTTGGCGGAGTACCTATCGTGGGCAAGATATTGAATACATAGAAATTCGATC
  CGATGCTGATGAAAATGTATCAGCTTCTGATAAAAGGCGAAATTACAACTACCGAGTGGTGATGCTGAAG
  GGGGATACAGCCTTGGACATGCGCGGACGATGCAGTGCTGGACAGAAGGTGCTGGCCTCTCTCATCATCC
  GACTGGCCCTGGCCGAAACCTTCTGTCTGAACTGCGGCATCCTTGCCTTGGATGAGCCTACAACAAACCT
  GGACCGAGAAAACATCGAGTCTCTGGCACATGCTTTGGTTGAGATTATAAAAAGTCGCTCGCAGCAGCGC
  AACTTCCAGCTTCTGGTCATCACTCACGATGAAGATTTTGTGGAGCTCCTAGGACGATCTGAGTATGTGG
  AGAAATTCTACAGAGTGAAGAAGAACATCGACCAGTGCTCAGAGATTGTCAAGAGCAGCATCAACTCTCT
  GGGCTCTTATGTTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201717 representing NM_022246
  Red=Cloning site Green=Tags(s)
  
  MSRIEKMSTLGVRSFGIEDKDKQIISFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDP
  KVAQETDVRAQIRLQFRDVNGEMVLVQRSMLCSQKSKKTEFKTLEGVITRIKHGEKVSLSSKCAEIDREM
  ISCLGVSKSVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALDTLRQVRQTQGQKVKECQTEL
  KYLRQNKEKACEIRDQITSKEAQLASSREIVKAYENELEPLKNRLKEIEHNLSKIMRLDNEIKALDSRKK
  QMEKDNSELEQKMEKVFQGTDEQLNDLYHNHQRTVREKERRLVDCQRELEKLSKEARLLNQERAELLVEQ
  GRLQLQADRHQEHIRARDSLIQSLAAHLELDGFERGPFSERQIKNFHELVRERQEREAKTASQLLSDLTD
  KEALKQRQMDEMRDKKSGLGRMIELKTEILTKKQTELRNVRNELQQLEGSSDRILELDQELTKAERELSK
  AEKNSSIETLKAEILNLQSEKADLDRNLRKLDQEMEQLNHHTTTRTQMEMLTKDKTDKDEQIRKIKSRHS
  DELTSLLGYFPNKKQLEDWLHSKSKEINQTRDRLAKLNKELASAEQNKNHINNELKKKEEQLSSYEDKLF
  DVCGSQDFESDLDRLKEDIEKSSKQRAMLAGATAVYSQFITQLTDENQSCCPGCQRVFQTEAELQEVISD
  LQSKLRLAPDKLKSTESELKKKERRRDEMLGLVPMRQSIIDLKEKEIPELRNRLQSVNRDIQRLKNDIEE
  QETLLGTVMPEEESAKVCLTDVTIMERFQMELKDVERKIAQQAAKLQGVDLDRTVQQVNQEKQEKQHKLD
  TVSSKIELNRKLIQDQQEQIQHLKSKTNELKSEKLQIATNLQRRQQMEEQTVELSTEVQSLNREIKDAKE
  QINPLEIALEKLQQEKEELIHRKNTSNKMAQDKINDIKEKVKNIHGYMKDIENYIQDGKDDYKKQKETEL
  NEVVIQLNECDKHKEKINKEMGTMRQDIDTKKIQERWLQDNLTLRKRREELKEVEEERKQHLKEMGQMQV
  LQMKNEHQKLEENIDTIKRNHSLALGRQKGYEEEILHFKKELREPQFRDAEEKYREMMIVMRTTELVNKD
  LDIYYKTLDHAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENVSASDKRRNYNYRVVMLK
  GDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSQQR
  NFQLLVITHDEDFVELLGRSEYVEKFYRVKKNIDQCSEIVKSSINSLGSYVH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022246
• ORF Size: 3936 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022246.1, NP_071582.1
• RefSeq Size: 4444
• RefSeq ORF: 3939
• Locus ID: 64012
• MW: 153.8 kDa
• Gene Summary: involved in DNA double-strand break repair [RGD, Feb 2006]