Mgat1 (NM_030861) Rat Tagged ORF Clone

CAT#: RR201823

Mgat1 (Myc-DDK-tagged ORF) - Rat mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (Mgat1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mgat1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201823 representing NM_030861
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGAAGCAGTCTGCAGGGCTTGTGCTTTGGGGTGCTATCATCTTTGTGGGCTGGAATGCCCTGC
  TGCTCCTCTTCTTCTGGACACGCCCAGCACCTGGCAGGCTGCCCTCAGACAGTGCCCTTGGTGATGACCC
  TGCCAGCCTCACCCGTGAGGTCATCCACCTGGCCGAGGACGCCGAGGCGGAGTTGGAGCGGCAGCGGGGA
  CTACTGCAGCAGATCAAGGAGCATTATTCTCTGTGGAGGCAGAGGTGGAGAGTTCCCACCGTGGCCCCTC
  CAGCCTGGCCCCGTGTGCCTGGGACCCCCTCACCAGCTGTGATCCCCATTCTGGTCATTGCCTGTGACCG
  CAGCACTGTCCGGCGCTGCCTGGATAAGTTGTTGCACTATCGGCCCTCTGCTGAGCATTTCCCCATTATT
  GTCAGTCAAGACTGTGGGCATGAAGAGACAGCACAGGTCATTGCTTCTTATGGCACCGCTGTCACACACA
  TCCGGCAGCCAGACCTGAGTAACATTGCCGTGCAGCCAGACCACCGTAAGTTCCAGGGTTACTACAAGAT
  TGCCAGGCACTACCGCTGGGCGCTAGGCCAGATCTTCAACAAGTTCAAGTTCCCGGCTGCTGTGGTAGTG
  GAGGATGATCTGGAAGTGGCGCCGGACTTCTTCGAGTACTTCCAGGCCACCTACCCGCTGCTGAAAGCAG
  ACCCCTCCCTTTGGTGTGTGTCGGCTTGGAATGATAATGGTAAGGAGCAGATGGTGGACTCCAGCAAACC
  TGAGCTGCTCTATCGAACAGACTTTTTTCCTGGCCTTGGATGGCTGCTGTTGGCTGATCTCTGGGCAGAA
  CTAGAGCCCAAGTGGCCCAAGGCCTTCTGGGACGACTGGATGCGCAGACCTGAGCAGCGGAAGGGGCGGG
  CTTGTATTCGTCCAGAAATTTCAAGAACTATGACATTTGGTCGCAAGGGTGTGAGCCATGGGCAGTTCTT
  TGACCAGCATCTTAAATTCATCAAGCTGAACCAGCAGTTCGTGCCCTTCACCCAGTTGGACCTGTCGTAC
  CTGCAGCGGGAGGCCTATGACCGGGACTTCCTTGCCCAGGTCTATGGTGCCCCCCAGCTGCAGGTGGAGA
  AAGTGAGGACCAATGATCGGAAGGAACTGGGGGAGGTGCGGGTACAGTACACTAGCAGAGACAGCTTTAA
  GGCCTTTGCTAAGGCCCTGGGTGTCATGGACGACCTCAAGTCTGGTGTCCCCAGAGCTGGCTACCGTGGC
  ATTGTCACTTTCCAGTTCCGGGGTCGGCGTGTCCACCTGGCACCCCCAGAGACATGGAATGGCTATGATC
  CTAGCTGGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201823 representing NM_030861
  Red=Cloning site Green=Tags(s)
  
  MLKKQSAGLVLWGAIIFVGWNALLLLFFWTRPAPGRLPSDSALGDDPASLTREVIHLAEDAEAELERQRG
  LLQQIKEHYSLWRQRWRVPTVAPPAWPRVPGTPSPAVIPILVIACDRSTVRRCLDKLLHYRPSAEHFPII
  VSQDCGHEETAQVIASYGTAVTHIRQPDLSNIAVQPDHRKFQGYYKIARHYRWALGQIFNKFKFPAAVVV
  EDDLEVAPDFFEYFQATYPLLKADPSLWCVSAWNDNGKEQMVDSSKPELLYRTDFFPGLGWLLLADLWAE
  LEPKWPKAFWDDWMRRPEQRKGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVPFTQLDLSY
  LQREAYDRDFLAQVYGAPQLQVEKVRTNDRKELGEVRVQYTSRDSFKAFAKALGVMDDLKSGVPRAGYRG
  IVTFQFRGRRVHLAPPETWNGYDPSWN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030861
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_030861.1, NP_110488.1
• RefSeq Size: 2546
• RefSeq ORF: 1344
• Locus ID: 81519
• MW: 51.6 kDa
• Gene Summary: enzyme involved in N-linked carbohydrate formation [RGD, Feb 2006]