Slc39a8 (NM_001011952) Rat Tagged ORF Clone

CAT#: RR201903

Slc39a8 (Myc-DDK-tagged ORF) - Rat solute carrier family 39 (metal ion transporter), member 8 (Slc39a8), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc39a8
• Synonyms: MGC108852
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201903 representing NM_001011952
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCGGGCCGGGCAGTGGCCGGGCTGCTGCTACTGGCGGCCACCGGCCTGGGACGCCCCTCGGAGG
  GGCCGGAGCTCGCCTTCACTGAGGATGTGCTGAGAGTGTTCGGCGCCAACCAGAGCCTGTCGGCCGCGCA
  GCTGGGGCGCCTGCTGGAGCGCCTGGGGGCTGCACCCCAGCAGGGAGCGCTGGAGCTGGGCCAGCTCCAC
  TTCAACCAGTGTCTGTCAGCGGAAGACATCTTTTCTCTTCATGGTTTCTCAAACGTCACCCAGATAACCA
  GCTCGAACTTCACTGCCATCTGCCCTGCGATCTTACAGCAGCTGAACTTTCATCCCTGTGAGGATCCACA
  GAAGCACAGCGTAAAGCCCAGTTTTTCCGAAGTCTGGGGCTATGGATTCTTGTCAGTGACAATCATCAAT
  TTGGCCTCTCTCCTGGGATTGATTTTAACCCCCTTGATAAAGAAGTCGTATTTCCCCAAGATTTTGACTT
  ATTTTGTGGGGCTGGCTATTGGGACCCTTTTCTCAAATGCCATTTTCCAGCTTATTCCAGAGGCATTTGG
  ATTTAATCCCAAAATTGACAATTATGTTGAAAAGGCAGTTGCTGTGTTTGGTGGATTTTACATGCTTTTC
  TTTGTTGAAAGAACACTTAAGATGCTACTGAAAACGTACGGCCAGAATGACCATACTCACTTCAGGAATG
  ATGACTTCGGTTCTAAAGAAAAAGCCCATCAACCCAAAACGTTGCCATTACCTCCTGTCAATGGAGTGAC
  ATGCTATGCCAACCCCGCTGTCACTGAGCCTAATGGACACATCCACTTCGACACTGTCAGTGTTGTGTCC
  CTGCAGGATGGAAAAGCAGAGTCAAGTTCATGCACCTGTTTGAAGGGGCCCAAACTGTCAGAAATAGGAA
  CGATTGCTTGGATGATCACGCTCTGTGATGCCCTTCACAATTTCATTGATGGCTTGGCGATCGGGGCATC
  CTATACTCTGTCTCTTCTTCAAGGACTCAGTACTTCCATAGCGATCCTGTGTGAGGAGTTTCCTCATGAG
  TTAGGGGACTTTGTGATCTTGCTCAATGCAGGGATGAGCACTCGGCAAGCCTTGCTGTTTAACTTCCTCT
  CTGCATGTTCCTGCTACGTTGGGCTTGCTTTCGGCATTTTGGTGGGCAACAACTTTGCTCCAAATATTAT
  ATTTGCACTTGCTGGAGGCATGTTCCTTTACATTTCTCTGGCAGATATGTTTCCAGAGATGAACGACATG
  CTGAGAGAAAAAGTAACTGGAAGACAAACGGACTTCACCTTCTTCATGATCCAGAACGCAGGGATGTTAA
  CCGGATTCACTGCCATCTTGCTCATCACCTTGTACGCAGGGGACATCGAATTGCAGCCGGATTCACTGCC
  ATCTTGCTCATCACCTTGTACGCAGGGGACATCGAATTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201903 representing NM_001011952
  Red=Cloning site Green=Tags(s)
  
  MAPGRAVAGLLLLAATGLGRPSEGPELAFTEDVLRVFGANQSLSAAQLGRLLERLGAAPQQGALELGQLH
  FNQCLSAEDIFSLHGFSNVTQITSSNFTAICPAILQQLNFHPCEDPQKHSVKPSFSEVWGYGFLSVTIIN
  LASLLGLILTPLIKKSYFPKILTYFVGLAIGTLFSNAIFQLIPEAFGFNPKIDNYVEKAVAVFGGFYMLF
  FVERTLKMLLKTYGQNDHTHFRNDDFGSKEKAHQPKTLPLPPVNGVTCYANPAVTEPNGHIHFDTVSVVS
  LQDGKAESSSCTCLKGPKLSEIGTIAWMITLCDALHNFIDGLAIGASYTLSLLQGLSTSIAILCEEFPHE
  LGDFVILLNAGMSTRQALLFNFLSACSCYVGLAFGILVGNNFAPNIIFALAGGMFLYISLADMFPEMNDM
  LREKVTGRQTDFTFFMIQNAGMLTGFTAILLITLYAGDIELQPDSLPSCSSPCTQGTSNC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011952
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3097 bp
• RefSeq ORF: 1389 bp
• Locus ID: 295455
• Cytogenetics: 2q43
• MW: 50.2 kDa