Dnmt3b (NM_001003959) Rat Tagged ORF Clone

CAT#: RR201942

Dnmt3b (Myc-DDK-tagged ORF) - Rat DNA methyltransferase 3B (Dnmt3b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnmt3b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201942 representing NM_001003959
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGAGACAGCAGACATCTTAATGAAGAAGAGGGTGCTAGTGGGTATGAGGACTGTATCATTGTTA
  ATGGGAACTGTAGTGACCAGTCCTCGGACACGAAGGATGCTCCCTCACCCCCAGTCTTGGAGGCAATCTG
  CACAGAGCCAGTCTGTACACCAGAGACCAGAGGCCGCAGATCAAGCTCACGGCTGTCTAAGAGGGAGGTC
  TCCAGCCTTCTGAATTACACGCAGGACGTGGTAGGAGATGGAGATGGTGAAGCGGATGATGGAGATGGCT
  CTGATATTCTCATGATGCCAAAGCTCACCCGAGAGACCAAGGATGCCAGGACTCCCTCTGAAAGCCCAGC
  CGTCCGAACCCGAAACAGCAACAGTATCTCCAGCCTGGAGAGGCAAAGAACCTCCCCCAGAATCACGCGA
  GGCCGGCAGGGCCGCTACCACGTTCAGGAGTACCCCGTGGAGTTCCCAGCTACCAAGTCTCGGAGGCGGC
  GAGCATCGTCTTCAGCAAGCACGCCATGGTCATCCCCTGCCAGCATCGAGCTCATGGAAGATGTGACACC
  TAAGAGCAGCAGTACGCCATCGGTTGACTTGAGCCAGGACGGCCCTCAGGAGGGCATGGATGCCACACAG
  GTGGATGCGGAGAGCAGGGATGGTGACAGCACTGAGTATCAGGATGACAAGGAGTTTGGAATAGGTGACC
  TTGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCTGCCATGGTGGTGTCCTGGAAAGCCACCTCCAA
  GCGCCAGGCCATGCCCGGAATGCGCTGGGTACAGTGGTTTGGCGATGGCAAGTTTTCTGAGATCGCTGCT
  GACAAGCTGGTGGCTCTGGGTCTGTTCAGCCAGCACTTTAATCTGGCCACCTTCAATAAGCTGGTTTCTT
  ATAGGAAGGCCATGTACCACACTCTGGAGAAAGCCATGGTGCGAGCTGGCAAGACCTTCCCCAGCCGCCC
  TGGAGACTCACTGGAGGACCAGCTGAAGCCCATGCTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGG
  ATCGAGGGCCTCAAACCCAACAACAAGCAACCAGAGGTTCATAAGTCGAAGGTGCGTCGTTCAGGCAGTA
  GGAACTTAGAAGCCAGGAGACGCGAGAACAAAAGTCGAAGACGCACAACCATTGACTTTGCCGCTTCTGA
  GTACTCCACACCCCCTAAGCGCCTCAAGACAAATAGCTATGGTGGGAAGGACCGAGGGGAAGATGAGGAG
  AGCCGAGAACGGATGGCTTCTGATGTCACTAACAACAAGGGCAATCTGGAAGACCGCTGTCTGTCCTGCG
  GTAAGAAGAACCCTGTGTCCTTCCACCCCCTCTTTGAGGGTGGGCTCTGTCAGAGTTGCCGGGATCGCTT
  CCTGGAGCTCTTCTACATGTACGATGAGGACGGCTATCAGTCCTACTGCACCGTGTGCTGTGAGGGCCGT
  GAACTGCTTCTGTGCAGTAACACAAGCTGCTGCAGGTGCTTCTGTGTGGAGTGTCTGGAGGTGCTGGTGG
  GTACAGGCACAGCGGAGGATGCCAAGCTGCAGGAACCCTGGAGCTGCTATATGTGCCTCCCGCAGCGCTG
  CCATGGGGTCCTGCGGCGCAGGAAGGACTGGAACATGCGCCTGCAAGACTTCTTCACTACTGATCCTGAC
  CTGGAAGAGTTTGAGCCGCCCAAGTTGTACCCAGCGATTCCTGCAGCCAAAAGGAGGCCCATTAGGGTCC
  TGTCCCTGTTTGATGGAATTGCAACAGGGTACTTGGTGCTCAAGGACTTGGGTATTAAAGTGGAGAAGTA
  CGTTGCCTCTGAAGTCTGTGCAGAGTCCATTGCTGTAGGAACCATTAAGCATGAAGGCCAAATCAAATAT
  GTCAATGACGTCCGGAAAATCACCAAGAAAAATATTGAAGAGTGGGGCCCATTTGACTTGGTGATTGGCG
  GAAGCCCATGCAATGATCTTTCTAATGTCAATCCTGCCAGGAAAGGCCTGTATGAGGGCACAGGAAGGCT
  CTTCTTTGAGTTTTACCACTTGCTGAATTATACACGCCCCAAGGAGGGCGACAACCGTCCATTCTTCTGG
  ATGTTCGAGAATGTTGTGGCCATGAAAGTGAATGACAAGAAAGACATCTCCCGATTCCTGGCATGTAACC
  CAGTGATGATCGACGCCATCAAGGTTTCTGCTGCTCACAGGGCCCGGTACTTCTGGGGTAATCTACCTGG
  AATGAACAGGCCTGTGATAGCTTCAAAGAATGATAAGCTCGAGCTACAAGACTGCTTGGAGTTCAGTAGG
  ACAGCAAAGTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTCCATCAGACAGGGGAAAAACCAGC
  TTTTCCCTGTAGTCATGAATGGCAAGGATGACGTTCTGTGGTGCACTGAGCTCGAAAGGATCTTCGGGTT
  CCCAGCTCACTACACAGACGTGTCCAACATGGGCCGGGGCGCCCGCCAGAAGCTACTGGGAAGGTCCTGG
  AGTGTGCCAGTCATCAGACACCTGTTTGCCCCCTTGAAGGACTACTTTGCATGTGAAAGTGTGCCAGTCA
  TCAGACACCTGTTTGCCCCCTTGAAGGACTACTTTGCATGTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201942 representing NM_001003959
  Red=Cloning site Green=Tags(s)
  
  MKGDSRHLNEEEGASGYEDCIIVNGNCSDQSSDTKDAPSPPVLEAICTEPVCTPETRGRRSSSRLSKREV
  SSLLNYTQDVVGDGDGEADDGDGSDILMMPKLTRETKDARTPSESPAVRTRNSNSISSLERQRTSPRITR
  GRQGRYHVQEYPVEFPATKSRRRRASSSASTPWSSPASIELMEDVTPKSSSTPSVDLSQDGPQEGMDATQ
  VDAESRDGDSTEYQDDKEFGIGDLVWGKIKGFSWWPAMVVSWKATSKRQAMPGMRWVQWFGDGKFSEIAA
  DKLVALGLFSQHFNLATFNKLVSYRKAMYHTLEKAMVRAGKTFPSRPGDSLEDQLKPMLEWAHGGFKPTG
  IEGLKPNNKQPEVHKSKVRRSGSRNLEARRRENKSRRRTTIDFAASEYSTPPKRLKTNSYGGKDRGEDEE
  SRERMASDVTNNKGNLEDRCLSCGKKNPVSFHPLFEGGLCQSCRDRFLELFYMYDEDGYQSYCTVCCEGR
  ELLLCSNTSCCRCFCVECLEVLVGTGTAEDAKLQEPWSCYMCLPQRCHGVLRRRKDWNMRLQDFFTTDPD
  LEEFEPPKLYPAIPAAKRRPIRVLSLFDGIATGYLVLKDLGIKVEKYVASEVCAESIAVGTIKHEGQIKY
  VNDVRKITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYTRPKEGDNRPFFW
  MFENVVAMKVNDKKDISRFLACNPVMIDAIKVSAAHRARYFWGNLPGMNRPVIASKNDKLELQDCLEFSR
  TAKLKKVQTITTKSNSIRQGKNQLFPVVMNGKDDVLWCTELERIFGFPAHYTDVSNMGRGARQKLLGRSW
  SVPVIRHLFAPLKDYFACESVPVIRHLFAPLKDYFACE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003959
• ORF Size: 2577 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 4289 bp
• RefSeq ORF: 2580 bp
• Locus ID: 444985
• Cytogenetics: 3q41
• MW: 97 kDa