Hfe (NM_053301) Rat Tagged ORF Clone

CAT#: RR201949

Hfe (Myc-DDK-tagged ORF) - Rat hemochromatosis (Hfe), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hfe
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201949 representing NM_053301
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGATCAGCTGGGCTCCCTGTGCGGCTGCTATTGCTGCTGCTGTTGTTGCTGCTGTGGTCCGTGG
  CCCCGCAGGCGCTGCGGCCCGGTTCACATTCTCTACGATATCTCTTCATGGGTGCCTCAAAGCCAGACCT
  CGGGCTGCCTTTCTTTGAGGCTCTGGGTTATGTGGATGACCAGCTCTTTGTATCCTACAATCACGAGAGT
  CGCCGTGCTGAGCCCAGGGCCCCATGGATCTTGGGGCAGACATCAAGCCAGCTGTGGCTGCAGCTGAGTC
  AGAGCCTGAAAGGGTGGGATTACATGTTCATAGTGGACTTCTGGACCATCATGGGCAACTATAACCACAG
  TAAGGTCACGAAGTTGAGAGTGGTGCCTGAGTCTCACATCCTGCAGGTGATCCTAGGATGTGAGGTGCAT
  GAAGACAACAGTACCAGTGGCTTCTGGAAATATGGCTACGATGGGCAAGATCACCTTGAATTCTGCCCCA
  AGACACTGAACTGGAGTGCAGCCGAGCCAAGGGCCTGGGCCACCAAGATGGAGTGGGAAGAGCACAGGAT
  CCGTGCCAGACAGAGCAGGGACTACCTGCAGAGGGACTGCCCCCAGCAGCTGAAGCAGGTCCTGGAGCTC
  CAGAGAGGGGTTCTGGGACAGCAAGTGCCTACTTTGGTGAAAGTGACTCGCCACTGGGCCTCTACAGGGA
  CCTCTCTAAGGTGTCAGGCTCTGAATTTCTTCCCCCAGAACATCACTATGAGGTGGTTGAAGGACAGCCA
  GCCCCTAGATGCCAAGGATGTCAACCCTGAGAACGTGCTGCCAAATGGGGATGGGACCTATCAGGGCTGG
  CTGACCTTGGCTGTGGCCCCTGGAGAAGAGACAAGGTTCAGCTGTCAAGTGGAGCACCCAGGCCTGGATC
  AGCCTCTCACTGCCACTTGGGAGCCCTCACGGTCTCAGGACATGATTATTGGAATCATAAGTGGGATCAC
  CATTTGTGCCATCTTCTTTGTTGGAATTCTGATCCTAGTCTTAAGGAAAAGGAAGGTTTCAGGAGGAACC
  ATGGGTGACTATGTCTTAACAGAGTGTGAGATGGGTGACTATGTCTTAACAGAGTGTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201949 representing NM_053301
  Red=Cloning site Green=Tags(s)
  
  MDRSAGLPVRLLLLLLLLLLWSVAPQALRPGSHSLRYLFMGASKPDLGLPFFEALGYVDDQLFVSYNHES
  RRAEPRAPWILGQTSSQLWLQLSQSLKGWDYMFIVDFWTIMGNYNHSKVTKLRVVPESHILQVILGCEVH
  EDNSTSGFWKYGYDGQDHLEFCPKTLNWSAAEPRAWATKMEWEEHRIRARQSRDYLQRDCPQQLKQVLEL
  QRGVLGQQVPTLVKVTRHWASTGTSLRCQALNFFPQNITMRWLKDSQPLDAKDVNPENVLPNGDGTYQGW
  LTLAVAPGEETRFSCQVEHPGLDQPLTATWEPSRSQDMIIGIISGITICAIFFVGILILVLRKRKVSGGT
  MGDYVLTECEMGDYVLTECE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053301
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq Size: 1670
• RefSeq ORF: 1083
• Locus ID: 29199
• MW: 41 kDa
• Gene Summary: membrane protein; may be involved with iron metabolism; defects in human homolog lead to hereditary hemochromotosis, an iron storage disorder [RGD, Feb 2006]