Acaca (NM_022193) Rat Tagged ORF Clone

CAT#: RR202137

Acaca (Myc-DDK-tagged ORF) - Rat acetyl-coenzyme A carboxylase alpha (Acaca), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acaca
• Synonyms: Acac; ACC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202137 representing NM_022193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAACCATCTCCGTTGGCCAAAACCCTGGAGCTGAACCAGCACTCCCGATTCATAATTGGGTCCG
  TGTCTGAAGACAACTCAGAAGATGAGATCAGTAACCTGGTAAAGCTGGACCTAGAGGAGAAGGAGGGCTC
  CCTGTCACCAGCCTCTGTCAGCTCAGATACACTTTCTGATTTGGGAATCTCTGCCTTACAGGATGGTTTG
  GCCTTTCACATGAGGTCCAGCATGTCCGGCTTGCACCTAGTAAAACAAGGTCGAGACAGAAAGAAAATAG
  ACTCTCAACGAGATTTCACTGTGGCTTCGCCAGCAGAATTTGTTACTCGTTTTGGGGGAAATAAAGTGAT
  TGAGAAGGTTCTTATCGCCAACAATGGTATTGCAGCAGTGAAATGCATGCGATCTATCCGGCGGTGGTCT
  TATGAAATGTTCCGCAATGAACGTGCCATCCGGTTTGTTGTCATGGTTACACCCGAAGACCTTAAAGCCA
  ATGCAGAATACATTAAGATGGCGGATCACTATGTTCCAGTGCCTGGAGGAGCAAACAACAACAATTATGC
  AAATGTGGAATTGATTCTTGATATTGCGAAAAGGATACCTGTACAGGCAGTGTGGGCTGGCTGGGGTCAT
  GCCTCCGAGAACCCCAAGCTCCCGGAGCTACTCTTAAAAAATGGCATTGCTTTCATGGGCCCTCCGAGTC
  AGGCCATGTGGGCGTTGGGGGATAAGATTGCATCGTCTATTGTGGCTCAAACTGCAGGTATCCCCACTCT
  TCCCTGGAGTGGCAGTGGTCTTCGAGTGGATTGGCAAGAAAATGATTTTTCGAAACGCATCTTAAATGTT
  CCACAGGATCTGTATGAGAAAGGCTATGTGAAGGATGTGGATGATGGACTGAAGGCAGCCGAGGAGGTTG
  GCTATCCAGTGATGATCAAGGCCTCAGAGGGAGGAGGAGGGAAGGGAATCAGAAAAGTTAACAATGCAGA
  TGACTTCCCTAACCTCTTCAGACAGGTTCAAGCTGAAGTCCCTGGATCACCTATATTTGTAATGAGATTA
  GCAAAACAGTCTCGTCATCTGGAGGTCCAGATTCTGGCGGATCAGTATGGCAATGCAATTTCTTTGTTTG
  GTCGTGACTGCTCTGTACAACGCAGGCATCAGAAGATCATTGAAGAAGCTCCTGCTGCTATTGCTACCCC
  AGCAGTATTTGAACACATGGAACAGTGTGCTGTAAAACTTGCCAAAATGGTTGGTTATGTGAGCGCTGGG
  ACTGTGGAATACTTGTACAGCCAGGACGGAAGCTTCTACTTCTTGGAACTGAACCCTCGGCTACAGGTTG
  AACATCCTTGTACAGAGATGGTGGCTGATGTCAATCTTCCTGCAGCACAGCTCCAGATTGCCATGGGGAT
  CCCTCTATTTAGGATCAAGGATATTCGTATGATGTATGGGGTATCTCCTTGGGGTGATGCTCCCATTGAT
  TTTGAAAATTCTGCTCATGTTCCTTGCCCAAGGGGCCATGTGATTGCTGCTCGGATCACCAGTGAAAACC
  CAGATGAGGGGTTTAAGCCCAGCTCTGGAACAGTTCAGGAACTTAATTTTCGCAGCAATAAGAATGTTTG
  GGGTTATTTCAGTGTTGCTGCTGCTGGGGGACTTCATGAATTTGCTGATTCTCAGTTCGGTCACTGCTTT
  TCCTGGGGAGAAAACAGAGAAGAAGCAATTTCAAATATGGTGGTGGCATTGAAGGAGCTGTCTATTCGGG
  GTGACTTTCGAACTACAGTAGAATACCTCATCAAGCTGCTGGAGACAGAAAGCTTTCAGTTGAACAGAAT
  CGACACTGGCTGGCTGGACAGACTGATTGCAGAGAAAGTGCAGGCAGAGCGTCCTGACACCATGTTGGGA
  GTTGTGTGTGGGGCTCTTCATGTGGCAGATGTGAACCTGAGAAATAGCATCTCTAACTTCCTTCACTCCT
  TAGAGAGGGGTCAAGTCCTTCCTGCTCACACACTTCTGAACACAGTAGATGTTGAACTTATCTATGAAGG
  AATCAAATATGTACTTAAGGTGACTCGGCAGTCTCCCAACTCCTACGTGGTGATAATGAACGGCTCGTGT
  GTGGAAGTGGACGTGCACCGGCTGAGTGATGGTGGACTGCTCTTGTCCTACGATGGCAGCAGTTACACCA
  CATACATGAAGGAGGAGGTAGACAGATACCGAATCACAATTGGCAATAAAACCTGTGTGTTTGAGAAGGA
  AAATGACCCGTCTGTAATGCGCTCCCCGTCTGCTGGGAAGTTAATCCAGTATATTGTGGAAGATGGAGGC
  CATGTGTTTGCTGGCCAGTGCTATGCAGAGATTGAGGTAATGAAGATGGTAATGACTTTGACAGCTGTAG
  AATCTGGCTGCATCCATTATGTCAAGCGACCTGGAGCAGCACTTGACCCAGGCTGTGTGATAGCCAAAAT
  GCAGCTGGACAATCCCAGTAAAGTTCAACAGGCTGAGCTTCACACGGGCAGTCTGCCCCAGATCCAGAGC
  ACAGCTCTCCGAGGCGAAAAGCTCCATCGAGTTTTCCACTATGTCCTGGATAACCTGGTCAATGTGATGA
  ATGGATACTGCCTTCCAGACCCTTTCTTCAGCAGCAAGGTAAAGGACTGGGTAGAACGGTTAATGAAGAC
  TCTGAGAGACCCCTCCCTGCCTCTTCTAGAATTGCAGGATATCATGACCAGTGTCTCTGGCCGGATCCCC
  CTCAACGTGGAGAAGTCTATTAAGAAGGAAATGGCTCAGTATGCTAGCAACATCACATCGGTCCTGTGTC
  AGTTTCCCAGCCAGCAGATTGCCAACATCCTAGATAGTCATGCAGCTACACTGAACCGGAAATCGGAGCG
  GGAAGTCTTCTTCATGAACACCCAGAGCATTGTCCAGCTGGTGCAGAGGTACCGAAGTGGCATCCGTGGC
  CACATGAAGGCTGTGGTGATGGATCTGCTCCGGCAGTACCTGCGGGTGGAGACACAGTTTCAGAATGGCC
  ACTACGACAAATGTGTATTCGCCCTTCGGGAAGAGAACAAAAGTGACATGAACACCGTACTGAACTACAT
  CTTCTCCCACGCCCAGGTCACCAAGAAGAATCTCCTGGTGACAATGCTTATTGATCAGTTGTGTGGCCGG
  GACCCTACACTTACTGATGAGCTGCTAAACATCCTCACAGAGCTAACTCAGCTCAGCAAAACCACCAACG
  CCAAAGTGGCACTGCGGGCTCGCCAGGTTCTTATTGCCTCCCATTTGCCATCGTACGACGTTCGCCATAA
  CCAAGTAGAGTCCATCTTCTTATCAGCCATCGACATGTATGGACACCAGTTTTGCATTGAGAACCTGCAG
  AAACTCATCCTATCAGAAACATCTATTTTCGATGTCCTCCCAAACTTTTTTTACCACAGCAACCAGGTGG
  TGAGGATGGCGGCTCTGGAGGTATATGTTCGAAGAGCTTATATCGCCTATGAGCTCAACAGTGTACAGCA
  TCGCCAGCTTAAGGACAACACCTGTGTGGTAGAATTTCAGTTCATGCTGCCCACATCTCATCCAAACAGA
  GGGAACATCCCCACGCTAAACAGAATGTCCTTTGCCTCCAACCTCAACCACTACGGCATGACTCATGTAG
  CTAGTGTCAGCGATGTTCTGTTGGACAACGCCTTCACACCACCTTGTCAACGCATGGGCGGGATGGTCTC
  TTTCCGGACCTTTGAAGATTTCGTCAGGATCTTTGATGAAGTAATGGGCTGCTTCTGCGACTCCCCACCC
  CAAAGCCCCACATTCCCAGAGTCCGGTCACACTTCACTCTATGATGAGGACAAGGTCCCCAGGGACGAAC
  CAATACATATTCTGAATGTGGCTATCAAGACTGATGGCGATATTGAGGATGACAGGCTTGCAGCTATGTT
  CAGAGAGTTCACCCAACAGAATAAAGCTACTCTGGTTGAGCATGGGATCCGGCGACTTACGTTCCTAGTT
  GCACAAAAGGATTTCAGAAAACAAGTCAACTGTGAGGTGGATCAGAGATTTCATAGAGAATTCCCCAAAT
  TTTTCACATTCCGAGCAAGGGATAAGTTTGAGGAGGACCGCATTTATCGACATCTGGAGCCTGCTCTGGC
  TTTCCAGTTAGAGCTGAACCGGATGAGAAATTTTGACCTTACTGCCATTCCATGCGCTAATCACAAGATG
  CACCTGTACCTTGGGGCTGCTAAGGTGGAAGTAGGCACAGAAGTGACTGACTACAGGTTCTTTGTTCGTG
  CGATCATCAGGCACTCTGATCTGGTCACGAAGGAAGCTTCTTTTGAATATCTACAAAATGAAGGAGAGCG
  ACTGCTCCTGGAAGCCATGGATGAATTGGAAGTTGCTTTCAATAACACAAATGTTCGCACAGACTGTAAC
  CATATATTCCTCAACTTTGTGCCCACAGTCATCATGGACCCATCAAAGATTGAAGAATCTGTGCGGAGCA
  TGGTAATGCGCTATGGAAGCCGGCTGTGGAAATTGCGGGTCCTCCAGGCAGAACTGAAAATCAACATTCG
  CCTGACAACAACTGGAAAAGCGATTCCCATCCGCCTCTTCCTGACAAACGAGTCTGGCTACTACTTGGAC
  ATCAGCCTGTATAAGGAAGTGACTGACTCCAGGACAGCACAGATCATGTTTCAGGCGTATGGAGACAAGC
  AGGGACCACTGCATGGAATGTTAATCAATACTCCGTATGTGACCAAAGACCTTCTTCAATCAAAGAGGTT
  TCAGGCACAGTCCTTGGGAACAACGTATATATATGATATCCCAGAGATGTTTCGGCAGTCGCTCATCAAG
  CTCTGGGAGTCCATGTCCACTCAAGCATTTCTTCCTTCACCCCCTTTGCCTTCCGACATACTGACGTATA
  CTGAACTGGTGTTGGATGATCAAGGCCAGCTTGTCCATATGAACAGACTTCCAGGAGGAAACGAGATTGG
  CATGGTAGCCTGGAAAATGAGCCTTAAAAGCCCTGAATATCCAGATGGCCGAGATGTCATTGTCATCGGC
  AATGACATTACATATCGGATTGGCTCCTTTGGGCCTCAGGAAGATTTGCTGTTTCTCAGAGCTTCTGAAC
  TTGCCAGAGCAGAAGGCATCCCACGCATCTACGTAGCAGCCAACAGTGGAGCTAGAATTGGACTGGCAGA
  AGAAATCCGTCATATGTTCCACGTGGCCTGGGTAGACTCTGAGGATCCTTACAAGGGATACAAGTATTTA
  TATCTGACACCCCAGGATTATAAAAGAGTGAGTGCTCTCAATTCTGTCCACTGTGAACATGTGGAAGATG
  AAGGAGAATCCAGGTACAAGATAACAGACATTATCGGGAAAGAAGAAGGACTTGGAGCAGAGAACCTTCG
  GGGTTCTGGAATGATTGCTGGGGAATCCTCATTGGCTTATGATGAGATCATCACCATCAGCCTGGTTACA
  TGCCGGGCCATTGGTATTGGGGCTTACCTTGTCCGGCTGGGACAAAGAACCATCCAGGTTGAGAACTCTC
  ACTTAATTCTAACAGGAGCCGGTGCCCTCAACAAAGTCCTTGGTCGGGAAGTATACACCTCCAACAATCA
  GCTTGGGGGCATCCAGATAATGCACAACAACGGAGTTACCCATTGCACTGTTTGTGATGACTTTGAGGGA
  GTGTTCACAGTCTTACACTGGCTGTCATACATGCCTAAGAACGTGCACAGTTCAGTTCCTCTCCTGAATT
  CCAAGGATCCTATAGATAGAATCATCGAGTTTGTTCCCACAAAGGCCCCGTATGATCCTCGGTGGATGCT
  GGCAGGCCGTCCTCACCCAACCCAGAAAGGCCAATGGTTGAGTGGATTTTTTGATTATGGCTCTTTCTCA
  GAAATCATGCAGCCCTGGGCGCAGACCGTGGTAGTTGGCAGAGCCAGGTTGGGGGGAATACCTGTGGGAG
  TAGTTGCTGTAGAAACCCGAACCGTGGAGCTCAGTGTACCAGCTGATCCTGCAAACCTGGATTCTGAAGC
  CAAGATAATCCAGCAGGCCGGCCAAGTTTGGTTTCCAGACTCTGCATTTAAGACCTATCAAGCTATCAAG
  GACTTTAACCGTGAAGGGCTACCTCTAATGGTCTTTGCCAACTGGAGAGGCTTCTCTGGTGGGATGAAAG
  ATATGTATGACCAGGTGCTCAAGTTTGGTGCTTATATTGTGGATGGCTTGCGGGAATGTTCCCAGCCTGT
  GATGGTCTACATTCCCCCACAGGCTGAGCTTCGGGGTGGTTCTTGGGTTGTGATCGACCCAACCATCAAT
  CCTCGGCACATGGAGATGTATGCTGACCGGGAAAGCAGGGGATCCGTTCTGGAACCAGAAGGGACAGTAG
  AAATCAAATTCCGCAAAAAGGATCTGGTGAAAACCATGCGTCGCGTAGACCCAGTCTACATCCGCTTGGC
  TGAGCGACTGGGCACCCCAGAGCTAAGCCCCACTGAGCGGAAGGAGCTGGAGAGCAAGTTGAAGGAGCGG
  GAGGAGTTCCTAATTCCCATTTACCATCAGGTAGCTGTGCAGTTTGCTGACTTGCACGACACCCCAGGCC
  GGATGCAGGAGAAGGGTGTCATTAATGATATCTTAGATTGGAAAACATCCCGCACCTTCTTCTACTGGCG
  ACTGAGGCGTCTCCTGCTGGAAGACCTGGTCAAGAAGAAAATCCACAGTGCCAACCCTGAGCTGACCGAT
  GGCCAGATCCAGGCCATGTTGAGACGCTGGTTTGTGGAAGTGGAAGGCACAGTGAAGGCTTACGTCTGGG
  ACAATAATAAGGATCTGGTGGAATGGCTGGAGAAGCAGCTGACAGAGGAAGATGGTGTCCGCTCTGTGAT
  AGAGGAGAACATCAAATACATCAGCAGGGACTATGTCCTCAAGCAGATCCGCAGCTTGGTGCAGGCCAAT
  CCAGAAGTTGCCATGGACTCCATCGTCCACATGACCCAGCACATCTCCCCCACTCAGCGAGCAGAGGTTG
  TAAGGATCCTTTCCACTATGGACTCCCCTTCTACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202137 representing NM_022193
  Red=Cloning site Green=Tags(s)
  
  MDEPSPLAKTLELNQHSRFIIGSVSEDNSEDEISNLVKLDLEEKEGSLSPASVSSDTLSDLGISALQDGL
  AFHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWS
  YEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGANNNNYANVELILDIAKRIPVQAVWAGWGH
  ASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNV
  PQDLYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRL
  AKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAG
  TVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLFRIKDIRMMYGVSPWGDAPID
  FENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCF
  SWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLG
  VVCGALHVADVNLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYEGIKYVLKVTRQSPNSYVVIMNGSC
  VEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGG
  HVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPQIQS
  TALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIP
  LNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRG
  HMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGR
  DPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYDVRHNQVESIFLSAIDMYGHQFCIENLQ
  KLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNR
  GNIPTLNRMSFASNLNHYGMTHVASVSDVLLDNAFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPP
  QSPTFPESGHTSLYDEDKVPRDEPIHILNVAIKTDGDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLV
  AQKDFRKQVNCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKM
  HLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCN
  HIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTTTGKAIPIRLFLTNESGYYLD
  ISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIK
  LWESMSTQAFLPSPPLPSDILTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMSLKSPEYPDGRDVIVIG
  NDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVAANSGARIGLAEEIRHMFHVAWVDSEDPYKGYKYL
  YLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVT
  CRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFEG
  VFTVLHWLSYMPKNVHSSVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFS
  EIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSVPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIK
  DFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECSQPVMVYIPPQAELRGGSWVVIDPTIN
  PRHMEMYADRESRGSVLEPEGTVEIKFRKKDLVKTMRRVDPVYIRLAERLGTPELSPTERKELESKLKER
  EEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHSANPELTD
  GQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQAN
  PEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022193
• ORF Size: 7035 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022193.1, NP_071529.1
• RefSeq Size: 7038
• RefSeq ORF: 7038
• Locus ID: 60581
• MW: 265.2 kDa
• Gene Summary: catalyzes the rate-limiting carboxylation of acetyl CoA to malonyl CoA during long-chain fatty acid synthesis [RGD, Feb 2006]