Chrna7 (NM_012832) Rat Tagged ORF Clone

CAT#: RR202164

Chrna7 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 7 (Chrna7), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrna7
• Synonyms: BTX; nAChRa7; NARAD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202164 representing NM_012832
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCGGCGGGCGGGGAGGCATCTGGCTGGCTCTGGCCGCGGCGCTGCTGCACGTGTCCCTGCAAGGCG
  AGTTCCAGAGGAGGCTGTACAAGGAGCTGGTCAAGAACTACAACCCGCTGGAGAGGCCGGTGGCCAACGA
  CTCGCAGCCGCTCACCGTGTACTTCTCCCTGAGTCTCCTGCAGATCATGGATGTGGATGAGAAGAACCAA
  GTTTTAACCACCAACATTTGGCTACAAATGTCTTGGACAGATCACTATTTGCAGTGGAACATGTCTGAGT
  ACCCCGGAGTGAAGAATGTTCGTTTTCCAGATGGCCAGATTTGGAAACCAGACATTCTCCTCTATAACAG
  TGCTGATGAGCGCTTTGATGCCACGTTCCACACCAATGTTTTGGTGAATGCATCTGGGCATTGCCAGTAT
  CTCCCTCCAGGCATATTCAAGAGCTCCTGCTACATTGACGTTCGCTGGTTCCCTTTTGATGTGCAGCAGT
  GCAAACTGAAGTTTGGGTCCTGGTCCTATGGAGGGTGGTCACTGGACCTGCAAATGCAAGAGGCAGATAT
  CAGCAGCTATATCCCCAACGGAGAATGGGATCTCATGGGAATCCCTGGCAAAAGGAATGAGAAGTTCTAT
  GAGTGCTGCAAAGAGCCATACCCAGATGTCACCTACACAGTAACCATGCGCCGTAGGACACTCTACTATG
  GCCTCAATCTGCTCATCCCTTGTGTACTCATTTCAGCCCTGGCTCTGCTGGTATTCTTGCTGCCTGCAGA
  CTCTGGAGAGAAAATCTCTCTTGGAATAACTGTCTTACTTTCTCTGACTGTCTTCATGCTGCTTGTGGCT
  GAGATCATGCCAGCAACATCTGATTCTGTGCCCTTGATAGCACAATACTTCGCCAGCACCATGATCATCG
  TGGGCCTCTCTGTAGTGGTGACAGTGATTGTGCTGAGATATCACCACCATGACCCTGATGGTGGCAAAAT
  GCCTAAGTGGACCAGAATCATTCTCCTGAACTGGTGTGCATGGTTTCTGCGCATGAAGAGGCCCGGAGAG
  GACAAGGTGCGGCCAGCTTGTCAGCACAAGCCTCGGCGCTGCAGCCTGGCCAGTGTGGAGCTGAGTGCAG
  GTGCTGGGCCACCCACCAGCAATGGCAACCTGCTCTACATTGGCTTCCGAGGCCTGGAGGGCATGCACTG
  TGCCCCAACTCCAGACTCTGGGGTCGTATGTGGCCGTTTGGCCTGCTCCCCAACACATGATGAGCACCTC
  ATGCACGGTGCACACCCCTCTGATGGGGACCCCGACCTGGCCAAGATCCTGGAGGAGGTCCGCTACATCG
  CCAACCGCTTCCGCTGCCAGGACGAGAGTGAGGTGATCTGCAGTGAATGGAAGTTTGCAGCCTGCGTGGT
  GGACCGCTTGTGCCTCATGGCCTTTTCGGTCTTTACCATCATCTGTACCATCGGCATCCTCATGTCAGCT
  CCAAACTTTGTGGAGGCTGTGTCCAAAGACTTTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202164 representing NM_012832
  Red=Cloning site Green=Tags(s)
  
  MCGGRGGIWLALAAALLHVSLQGEFQRRLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQ
  VLTTNIWLQMSWTDHYLQWNMSEYPGVKNVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNASGHCQY
  LPPGIFKSSCYIDVRWFPFDVQQCKLKFGSWSYGGWSLDLQMQEADISSYIPNGEWDLMGIPGKRNEKFY
  ECCKEPYPDVTYTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVA
  EIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLRYHHHDPDGGKMPKWTRIILLNWCAWFLRMKRPGE
  DKVRPACQHKPRRCSLASVELSAGAGPPTSNGNLLYIGFRGLEGMHCAPTPDSGVVCGRLACSPTHDEHL
  MHGAHPSDGDPDLAKILEEVRYIANRFRCQDESEVICSEWKFAACVVDRLCLMAFSVFTIICTIGILMSA
  PNFVEAVSKDFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012832
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012832.1, NM_012832.2, NM_012832.3, NP_036964.3
• RefSeq Size: 1509
• RefSeq ORF: 1509
• Locus ID: 25302
• MW: 56.5 kDa
• Gene Summary: subunit of nicotinic acetylcholline receptor; may mediate nicotine induced increase in tyrosine hydroxylase expression; may play a role in neuroprotection [RGD, Feb 2006]