Ptdss1 (NM_001012113) Rat Tagged ORF Clone

CAT#: RR202286

Ptdss1 (Myc-DDK-tagged ORF) - Rat phosphatidylserine synthase 1 (Ptdss1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptdss1
• Synonyms: PSS-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202286 representing NM_001012113
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCTGCGTAGGGAGCAGGACTCTGAGCAAGGATGATGTGAACTACAGGATGCATTTCCGCATGA
  TCAACGAGCAGCAAGTGGAGGACATCACCATAGACTTCTTCTACCGGCCGCACACCATCACCCTGCTCAG
  CTTTACCATCATCAGCCTCATGTACTTCGCCTTCACCAGGGATGACTGTGTTCCTGAAGACAACATCTGG
  AGAGGCATCCTTTCCGTTATCTTCTTCTTCCTCATCATCAGCGTGTTAGCATTCCCCAATGGCCCTTTTA
  CTCGGCCCCATCCAGCCTTATGGCGAATGGTTTTTGGCCTCAGTGTGCTGTACTTCCTCTTCTTGGTATT
  CCTCCTGTTCCTGAACTTCGAGCAGGTTAAATCCCTGATGTATTGGCTCGACCCCAATCTCCGATACGCC
  ACCAGAGAAGCAGACATCATGGAGTATGCCGTGAACTGTCATGTGATCACCTGGGAGAGAATCGTTAGTC
  ACTTTGATATATTTGCTTTTGGGCATTTCTGGGGCTGGGCCATGAAGGCCTTGTTGATCCGTAGTTATGG
  GCTCTGCTGGACAATCAGTATCACCTGGGAGCTGACAGAGCTTTTCTTCATGCATCTTCTGCCCAACTTT
  GCTGAGTGCTGGTGGGACCAGGTCATTCTGGACATCCTGTTGTGTAATGGTGGTGGAATCTGGCTGGGCA
  TGGTCGTTTGCCGGTTTTTGGAGATGAGGACTTACCACTGGGCAAGCTTCAAGGACATCCACACCACCAC
  AGGGAAGATCAAGCGTGCTGTGCTACAGTTCACCCCTGCTAGCTGGACCTACGTCAGGTGGTTTGACCCC
  AAGTCTTCCTTTCAGAGAGTGGCTGGTATATACCTTTTCATGATCATCTGGCAGCTGACTGAGTTGAATA
  CCTTCTTCTTGAAGCACATCTTTGTGTTCCAAGCCAGCCATCCCTTAAGCTGGTGCAGAATTCTCTTCAT
  CGGCTGCATTACGGCTCCCACAGTGAGACAGTACTACGCCTACCTCACGGACACACAGTGCAAGCGTGTG
  GGGACACAGTGCTGGGTATTTGGGGTCATTGGTTTCCTGGAAGCCATTGTTTGCATCAAATTTGGACAAG
  ATCTCTTCTCTAAGACCCAGATACTCTACGTGGTGCTTTGGCTTCTTTGTGTGGCCTTCACTACGTTCCT
  GTGTCTGTACGGCATGGTTTGGTATGCAGAGCACTATGGACACCGGGAAAAGACCTATTCAGAGTGCGAA
  GATGGCACTTACAGTCCAGAGATCTCTTGGTATCACGGGAAAGGCTCAAAAGGTTCTGAGGACAGCCCAC
  CGAAGCATTCAAACAACAACGAAAGCCATTCTTCCAGAAGAAGGAACCGGCATTCCAAGTCCAAAGTCAC
  CAATGGAGTTGGGAAGAAACAATGGAGTTGGGAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202286 representing NM_001012113
  Red=Cloning site Green=Tags(s)
  
  MASCVGSRTLSKDDVNYRMHFRMINEQQVEDITIDFFYRPHTITLLSFTIISLMYFAFTRDDCVPEDNIW
  RGILSVIFFFLIISVLAFPNGPFTRPHPALWRMVFGLSVLYFLFLVFLLFLNFEQVKSLMYWLDPNLRYA
  TREADIMEYAVNCHVITWERIVSHFDIFAFGHFWGWAMKALLIRSYGLCWTISITWELTELFFMHLLPNF
  AECWWDQVILDILLCNGGGIWLGMVVCRFLEMRTYHWASFKDIHTTTGKIKRAVLQFTPASWTYVRWFDP
  KSSFQRVAGIYLFMIIWQLTELNTFFLKHIFVFQASHPLSWCRILFIGCITAPTVRQYYAYLTDTQCKRV
  GTQCWVFGVIGFLEAIVCIKFGQDLFSKTQILYVVLWLLCVAFTTFLCLYGMVWYAEHYGHREKTYSECE
  DGTYSPEISWYHGKGSKGSEDSPPKHSNNNESHSSRRRNRHSKSKVTNGVGKKQWSWEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012113
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2547 bp
• RefSeq ORF: 1422 bp
• Locus ID: 314553
• Cytogenetics: 7q22
• MW: 55.7 kDa