Sardh (NM_053664) Rat Tagged ORF Clone

CAT#: RR202318

Sardh (Myc-DDK-tagged ORF) - Rat sarcosine dehydrogenase (Sardh), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sardh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202318 representing NM_053664
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCACTGAGCCGGGTCCTCCGTGTAGCTGCCACCTGTCCTCGAGGGAGATCTGCTCGCAACCTGG
  GGCTACAGCCCCTAGCCAGGGAGGCCAGGCCCACCACTGAGAAGAGTGTTCCATACCAGTGCACCCTGAA
  GGAGGAGGCCCAAGGTGCCTCTGTGGTGCCCCAAGGACCAAGCCAGCCCCTGCCTAGCACAGCCAATGTT
  GTGGTCATTGGTGGGGGCAGTTTGGGCTGCCAGACTCTGTACCACTTGGCCAAGCTGGGTGTAGGTGGAG
  TTGTGCTGCTGGAGAGAGAGCGACTGACCTCTGGGACCACCTGGCACACAGCAGGCCTACTGTGGCAGCT
  GCGGCCCAGCGATGTGGAAGTAGAGCTTCTGGCTCACACACGGCAAGTGGTGAGCCATGATCTGGAAGAG
  GAGACTGGGCTTCACACAGGCTGGATCCAGAATGGTGGCCTCTTCATCGCCTCCAACCAGCAGCGCCTGG
  ATGAATACAAGAGGCTCATGTCGTTGGGCAAGGCCTACGGTATAGAATCCCATGTGCTGAGCCCTGCAGA
  GACCAAGGATCTGTATCCGCTGATGAATGTGGACGACCTCTATGGGACCCTGTATGTGCCTCGGGATGGT
  ACCATGGACCCGGCTGGCACCTGCACTACCCTCACCAGAGCAGCAGTGGCTAGGGGAGCTCAGGTCATTG
  AAAACTGTGCAGTGACTGGCATCCGTGTGCGGACAGACGACTTTGGAGTGCGGCGGGTGACAGCTGTGGA
  GACTCAACATGGCTCCATCCAGACACCCTGCGTGGTCAACTGTGCAGGGGTGTGGGCGAGCTCCGTGGGC
  CGGATGGCTGGAGTGAAGGTCCCGCTGGTGGCCATGCACCATGCCTACGTTGTCACTGAGCGCATTGAGG
  GGATCCAGAACATGCCCAATGTCCGGGATCATGATGCTTCTGTCTACCTCCGCCTGCAGGGGGATGCCTT
  GTCTGTGGGTGGCTATGAGGCCAACCCCATCTTTTGGGATGAGGTGTCAGACAAATTTGCCTTTGGCCTC
  TTTGACCTGGACTGGGATGTGTTCACTCAGCACATTGAAGGTGCCATTAACCGTGTCCCCGTGTTGGAGA
  AAACGGGGATCAAGTCCACTGTCTGTGGCCCTGAATCCTTCACACCCGACCATAAGCCCCTGATGGGGGA
  GGCTCCTGAGCTCCGAGGCTTCTTCCTTGGCTGTGGCTTCAACAGTGCAGGCATGATGTTGGGTGGTGGC
  TGTGGACAGGAGCTGGCCCATTGGATTGTGCATGGGCGCCCAGAGAAGGACATGTACAGCTATGACATCA
  GGCGTTTCCACCACTCGCTCACGGACCACCCACGCTGGATCCGAGAGCGCAGCCACGAGTCCTATGCCAA
  GAACTACTCGGTTGTCTTTCCTCACGATGAGCCCCTGGCGGGCCGCAACATGAGGAGGGACCCTCTGCAC
  GAGGAGCTGCTTGGACAAGGCTGTGTGTTTCAAGAACGACAGGGCTGGGAACGGCCAGGATGGTTCAACC
  CCCAAGAAACAGCTCAGGTCCTAGACTACGACTACTATGGGGCCTACGGACACCAGGCACACAAGGACTA
  CGCCTACAGCAGGCTGCTGGGCGATGAATATACCTTCGACTTCCCACCCCACCACTGCGTGATCCAGAAG
  GAATGTCTGGCCTGCAGAACGGCTGCCGCCGTGTTCAACATGTCTTACTTCGGGAAGTTCTACCTTCTGG
  GGGCAGACGCCAGGAAGGCTCCAGACTGGCTCTTCTCAGCAGATGTCAACCGACCCCCAGGCTCCACGGT
  GTACACATGCATGCTGAACCAACGGGGAGGCACCGAGAGCGACCTGACTGTTAGCTGCCTGGCCCCTGGT
  GCCCAGGCCAGTCCCCTGGCCCCTGCCTTTGAAGGGGATGGCTACTACCTAGCCGTAGGTGGGGCTGTGG
  CCCAACATAACTGGTCCCACATAAACACGGTGCTGCAGGATCAGGAGTTCCGGTGCCAGCTTATGGATTG
  TTCTGAAGATCTGGGCATGCTCAGTATCCAGGGCCCAGCCAGCCGTGACATTCTCCAGGATGTGCTTGAT
  GCTGACCTGAGCAATGAAGCCTTCCCCTTCTCCACCCACCAGCTGGTGAGAGCTGCTGGACACCTGGTCC
  GAGCCATCCGGCTATCCTTTGTCGGGGAGCTGGGATGGGAGCTGCATGTGCCCCAAGCGTCCTGCTTGCC
  TGTGTACCGGGCTGTGATGGCTGCAGGGGCGAAGCACGGCCTAGTCAATGCAGGCTACCGTGCCATCGAC
  TCCCTGAGCATTGAGAAAGGCTATCGGCACTGGCATGCAGACCTGAGGTCCGATGACAGCCCCCTGGAGG
  CAGGGTTGGCCTTCACCTGCAAGCTCAAGACCTCAGTGCCCTTCCTAGGCCGAGAGGCCCTGGAGAAGCA
  GCGTGCCACAGGCCTCCGACGACGCCTGGTGTGCCTCACTGTGGAAGAGGAGGTTCCTATGTTCGGCCTA
  GAGGCCATCTGGAGAAATGGCCAAGTGGTGGGCCATGTCCGGAGAGCTGACTTTGGATTCACCGTGAACA
  AGACCATCGCCTATGGCTACATCCGAGACCCCAGTGGAGGGCCAGTCTCTCTGGACTTTGTGAAGAATGG
  GGACTATGCCTTGGAGAGAATGGGGGTCACCTATGCTGCCCAGGTTCACCTGAAGTCTCCCTTTGATCCT
  GACAACAAGCGGGTGAAGGGAATCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202318 representing NM_053664
  Red=Cloning site Green=Tags(s)
  
  MASLSRVLRVAATCPRGRSARNLGLQPLAREARPTTEKSVPYQCTLKEEAQGASVVPQGPSQPLPSTANV
  VVIGGGSLGCQTLYHLAKLGVGGVVLLERERLTSGTTWHTAGLLWQLRPSDVEVELLAHTRQVVSHDLEE
  ETGLHTGWIQNGGLFIASNQQRLDEYKRLMSLGKAYGIESHVLSPAETKDLYPLMNVDDLYGTLYVPRDG
  TMDPAGTCTTLTRAAVARGAQVIENCAVTGIRVRTDDFGVRRVTAVETQHGSIQTPCVVNCAGVWASSVG
  RMAGVKVPLVAMHHAYVVTERIEGIQNMPNVRDHDASVYLRLQGDALSVGGYEANPIFWDEVSDKFAFGL
  FDLDWDVFTQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSAGMMLGGG
  CGQELAHWIVHGRPEKDMYSYDIRRFHHSLTDHPRWIRERSHESYAKNYSVVFPHDEPLAGRNMRRDPLH
  EELLGQGCVFQERQGWERPGWFNPQETAQVLDYDYYGAYGHQAHKDYAYSRLLGDEYTFDFPPHHCVIQK
  ECLACRTAAAVFNMSYFGKFYLLGADARKAPDWLFSADVNRPPGSTVYTCMLNQRGGTESDLTVSCLAPG
  AQASPLAPAFEGDGYYLAVGGAVAQHNWSHINTVLQDQEFRCQLMDCSEDLGMLSIQGPASRDILQDVLD
  ADLSNEAFPFSTHQLVRAAGHLVRAIRLSFVGELGWELHVPQASCLPVYRAVMAAGAKHGLVNAGYRAID
  SLSIEKGYRHWHADLRSDDSPLEAGLAFTCKLKTSVPFLGREALEKQRATGLRRRLVCLTVEEEVPMFGL
  EAIWRNGQVVGHVRRADFGFTVNKTIAYGYIRDPSGGPVSLDFVKNGDYALERMGVTYAAQVHLKSPFDP
  DNKRVKGIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053664
• ORF Size: 2757 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053664.1, NM_053664.2, NP_446116.1
• RefSeq Size: 3069
• RefSeq ORF: 2760
• Locus ID: 114123
• MW: 101.4 kDa
• Gene Summary: a flavoenzyme involved in the oxidative degradation of choline to glycine [RGD, Feb 2006]