Ung (NM_001013124) Rat Tagged ORF Clone

CAT#: RR202410

Ung (Myc-DDK-tagged ORF) - Rat uracil-DNA glycosylase (Ung), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ung
• Synonyms: MGC108922
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202410 representing NM_001013124
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATCTTGGGGCCGCGGCCTTTGAAGTTGGCGCGTAGCCTGCGCGCGCCGCGGGGAGCCAGGCTGC
  GGAGCCTTACTCCGGACCCCGACTCCTGGCAGGCCAGCCCCGCCAAGAAGGCCCGGGTGGAGCAGGATGA
  GCCGGCCACACCGCCCTCTTCGCCGCTTAGCGCCGAGCAGCTCGTCCGCATCCAGAGGAACAAGGCCGCG
  GCGCTGCTCAGGCTCGCCGCCCGCAATGTGCCCGCCGGCCTCGGCGAGAGCTGGAAGCAGCAGCTGTGCG
  GGGAGTTCGGGAAGCCGTACTTCGTCAAGCTCATGGGCTTTGTTGCTGAAGAAAGGAAACACCACAAGGT
  CTATCCGCCCCCGGAGCAGGTGTTCACGTGGACCCAGATGTGCGACATCCGTGATGTGAAGGTTGTCATT
  CTGGGACAGGATCCATATCACGGACCTAATCAAGCTCATGGGCTCTGCTTCAGTGTCCAGAGGCCAGTTC
  CACCCCCGCCCAGTTTGGAAAACATTTTTAAAGAACTGTCTACAGACATCGATGGTTTTGTTCACCCTGG
  CCATGGGGATCTGTCAGGGTGGGCCAGACAAGGTGTCCTCCTCCTCAACGCCGTCCTCACTGTCCGCGCA
  CACCAAGCCAATTCCCATAAGGAGCGGGGCTGGGAGCAGTTCACGGATGCAGTTGTGTCGTGGCTGAATC
  AGAACCTGAATGGCCTTGTCTTCCTCCTCTGGGGCTCCTATGCTCAGAAGAAGGGCAGTGCCATCGACAG
  GAAGCGTCACCATGTTCTGCAGACAGCTCATCCCTCCCCGCTGTCGGTGTACAGAGGGTTCTTTGGATGT
  AGACATTTTTCTAAAGCCAATGAACTGCTCCAGAGGTCTGGCAAGAAACCCATCAGCTGGAAGGAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202410 representing NM_001013124
  Red=Cloning site Green=Tags(s)
  
  MGILGPRPLKLARSLRAPRGARLRSLTPDPDSWQASPAKKARVEQDEPATPPSSPLSAEQLVRIQRNKAA
  ALLRLAARNVPAGLGESWKQQLCGEFGKPYFVKLMGFVAEERKHHKVYPPPEQVFTWTQMCDIRDVKVVI
  LGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIFKELSTDIDGFVHPGHGDLSGWARQGVLLLNAVLTVRA
  HQANSHKERGWEQFTDAVVSWLNQNLNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFGC
  RHFSKANELLQRSGKKPISWKEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013124
• ORF Size: 909 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013124.1, NP_001013142.1
• RefSeq Size: 1855 bp
• RefSeq ORF: 912 bp
• Locus ID: 304577
• Cytogenetics: 12q16
• MW: 33.9 kDa