Cpt1a (NM_031559) Rat Tagged ORF Clone

CAT#: RR202656

Cpt1a (Myc-DDK-tagged ORF) - Rat carnitine palmitoyltransferase 1a, liver (Cpt1a), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cpt1a
• Synonyms: CPT-Ia
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202656 representing NM_031559
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGGCTCACCAAGCTGTGGCCTTCCAGTTCACTGTCACCCCCGATGGCATTGACCTCCGCCTGA
  GCCACGAAGCCCTCAAACAGATCTGCCTGTCGGGGCTGCACTCCTGGAAGAAGAAGTTCATCCGGTTCAA
  GAATGGCATCATCACTGGTGTGTTCCCCGCGAATCCGTCCAGCTGGCTTATCGTGGTGGTGGGTGTGATT
  TCATCCATGCATGCCAAAGTGGACCCCTCCCTGGGCATGATCGCAAAGATCAGTCGGACCCTAGACACCA
  CTGGCCGAATGTCAAGCCAGACGAAGAACATTGTGAGCGGCGTCCTCTTTGGTACAGGGCTCTGGGTGGC
  AGTCATCATGACCATGCGCTACTCGCTGAAGGTGCTGCTCTCCTACCACGGCTGGATGTTTGCAGAACAC
  GGCAAAATGAGCCGCAGCACCAAGATCTGGATGGCTATGGTCAAGGTCCTCTCAGGTCGGAAGCCCATGT
  TGTACAGCTTCCAGACGTCTCTGCCACGCCTGCCTGTCCCAGCTGTCAAAGATACTGTGAGCAGGTACCT
  GGAATCTGTGAGGCCACTGATGAAGGAAGAAGACTTCCAGCGCATGACAGCACTGGCCCAGGATTTTGCT
  GTCAACCTCGGACCCAAATTGCAGTGGTATTTGAAGCTAAAATCCTGGTGGGCCACAAATTACGTGAGTG
  ACTGGTGGGAAGAATATATCTACCTGCGGGGCCGAGGGCCGCTCATGGTCAACAGCAACTACTACGCCAT
  GGAGATGCTGTACATCACCCCAACCCATATCCAGGCAGCGAGAGCTGGCAACACCATCCACGCCATACTG
  CTGTATCGTCGCACATTAGACCGTGAGGAACTCAAACCCATTCGTCTTCTGGGATCCACCATTCCACTCT
  GCTCGGCCCAGTGGGAGCGACTCTTCAATACTTCCCGGATCCCTGGGGAAGAGACAGACACCATCCAACA
  TATCAAGGACAGCAGGCACATTGTTGTGTACCACAGAGGGCGGTACTTCAAGGTCTGGCTCTACCACGAT
  GGGAGGCTGCTGAGGCCCCGAGAGCTGGAGCAGCAGATGCAGCAGATCCTGGATGATCCCTCAGAGCCAC
  AGCCTGGGGAGGCCAAGCTGGCCGCCCTCACTGCTGCAGACAGAGTGCCCTGGGCAAAGTGTCGGCAGAC
  CTATTTTGCACGAGGGAAAAATAAGCAGTCCCTGGATGCGGTGGAAAAGGCAGCGTTCTTCGTGACGTTG
  GACGAATCGGAGCAGGGATACAGAGAGGAGGATCCTGAGGCATCCATCGACAGCTACGCCAAATCCCTGC
  TGCATGGAAGATGCTTTGACAGGTGGTTTGACAAGTCCATCACCTTTGTTGTCTTCAAAAACAGCAAGAT
  AGGCATAAATGCAGAGCACTCCTGGGCGGACGCGCCCGTCGTGGGCCATTTGTGGGAGTATGTCATGGCC
  ACCGACGTCTTCCAGCTGGGTTACTCAGAGGATGGACACTGTAAAGGAGACACCAACCCCAACATCCCTA
  AGCCCACAAGGCTACAATGGGACATTCCAGGAGAGTGCCAGGAGGTCATAGATGCATCCCTGAGCAGCGC
  CAGTCTTTTGGCAAATGATGTGGACCTGCATTCCTTCCCATTTGACTCTTTCGGCAAAGGCTTGATCAAG
  AAGTGCCGGACGAGTCCCGATGCCTTCATCCAGCTGGCGCTGCAGCTCGCACATTACAAGGACATGGGCA
  AGTTCTGCCTCACATATGAGGCCTCCATGACCCGGCTCTTCCGAGAAGGGAGGACAGAGACTGTACGCTC
  CTGCACTATGGAGTCCTGCAACTTTGTGCAGGCCATGATGGACCCCAAGTCAACGGCAGAGCAGAGGCTC
  AAGCTGTTCAAGATAGCTTGTGAGAAGCACCAGCACCTGTACCGCCTCGCCATGACGGGCGCCGGCATCG
  ACCGCCATCTCTTCTGCCTCTATGTGGTGTCCAAGTATCTTGCAGTCGACTCACCTTTCCTGAAGGAGGT
  ATTGTCTGAGCCATGGAGGTTGTCTACGAGCCAGACTCCTCAGCAGCAGGTGGAGCTCTTTGACTTTGAG
  AAAAACCCTGACTATGTGTCCTGTGGAGGGGGCTTTGGGCCGGTTGCCGATGACGGCTATGGTGTCTCCT
  ACATTATAGTGGGAGAGAATTTTATCCACTTCCATATTTCTTCCAAGTTCTCTAGCCCTGAGACAGACTC
  ACACCGCTTTGGGAAGCACTTGAGACAAGCCATGATGGACATTATCACCTTGTTTGGCCTCACCATCAAT
  TCTAAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202656 representing NM_031559
  Red=Cloning site Green=Tags(s)
  
  MAEAHQAVAFQFTVTPDGIDLRLSHEALKQICLSGLHSWKKKFIRFKNGIITGVFPANPSSWLIVVVGVI
  SSMHAKVDPSLGMIAKISRTLDTTGRMSSQTKNIVSGVLFGTGLWVAVIMTMRYSLKVLLSYHGWMFAEH
  GKMSRSTKIWMAMVKVLSGRKPMLYSFQTSLPRLPVPAVKDTVSRYLESVRPLMKEEDFQRMTALAQDFA
  VNLGPKLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYYAMEMLYITPTHIQAARAGNTIHAIL
  LYRRTLDREELKPIRLLGSTIPLCSAQWERLFNTSRIPGEETDTIQHIKDSRHIVVYHRGRYFKVWLYHD
  GRLLRPRELEQQMQQILDDPSEPQPGEAKLAALTAADRVPWAKCRQTYFARGKNKQSLDAVEKAAFFVTL
  DESEQGYREEDPEASIDSYAKSLLHGRCFDRWFDKSITFVVFKNSKIGINAEHSWADAPVVGHLWEYVMA
  TDVFQLGYSEDGHCKGDTNPNIPKPTRLQWDIPGECQEVIDASLSSASLLANDVDLHSFPFDSFGKGLIK
  KCRTSPDAFIQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTMESCNFVQAMMDPKSTAEQRL
  KLFKIACEKHQHLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEVLSEPWRLSTSQTPQQQVELFDFE
  KNPDYVSCGGGFGPVADDGYGVSYIIVGENFIHFHISSKFSSPETDSHRFGKHLRQAMMDIITLFGLTIN
  SKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031559
• ORF Size: 2319 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031559.1, NM_031559.2, NP_113747.2
• RefSeq Size: 4356
• RefSeq ORF: 2322
• Locus ID: 25757
• MW: 88.1 kDa
• Gene Summary: enzyme that catalyses the transfer of long chain fatty acids to carnitine for translocation across the mitochondrial inner membrane [RGD, Feb 2006]