Atp2b4 (NM_001005871) Rat Tagged ORF Clone

CAT#: RR202745

Atp2b4 (Myc-DDK-tagged ORF) - Rat ATPase, Ca++ transporting, plasma membrane 4 (Atp2b4), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp2b4
• Synonyms: PMCA4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202745 representing NM_001005871
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGAATCCATCAGGACATAACTTGCCAGCCAACTCGGTAGCTGAGAGCTATGAAGGGGAGTTTGGCT
  GCACCTTAATGGACCTGAGGAAGCTCATGGAGCTTCGTGCAGCTGATGCAGTGACCCAGATCAGTGCCCA
  CTATGGAAGTGTACAAGAAATCTGCGCAAGACTGAAAACCTCCCCTGTAGAAGGTCTGTCTGGGAACCCT
  GCAGATTTGGAGAAGCGTAGACTTGTTTTTGGGAAGAACATGATACCTCCGAAAAAGCCCAAGACGTTCT
  TAGAATTAGTGTGGGAAGCCCTGCAGGATGTCACACTCATCATCCTGGAGATCGCAGCCATCATATCCCT
  GGTCCTGTCCTTCTACCGGCCTCCTGGCGGGGAAAATGAAATCTGTGGCCACATTGTAAGTAACCCAGAA
  GAAGATGAGGAAGGAGAAACTGGCTGGATTGAAGGGGCAGCCATTCTTGCCTCGGTGATCATTGTGGTAT
  TCGTGACAGCCTTCAACGACTGGAGCAAAGAGAAGCAGTTCCGGGGGCTGCAGAGTCGCATTGAGCTTGA
  GCAAAAATTCTCCATTATCCGGAATGGTCAGCTTATACAACTCCCAGTGGCTGAGATTGTGGTGGGAGAT
  ATTGCCCAGGTCAAATACGGTGACCTGCTGCCTGCCGACGGAATCCTAATCCAGGGAAATGATCTGAAGA
  TTGACGAGAGCTCTTTGACAGGAGAATCAGATCATGTCAAGAAGACTCTGGACAAAGACCCCATGTTGCT
  CTCAGGGACTCATGTGATGGAAGGCTCTGGACGGATGGTGGTGACTGCTGTGGGAATCAATTCCCAGACT
  GGAATCATCTTCACCCTCTTAGGGGCTAATGAGGAAGAAGACGATGAGAAAAAGAAGAAAGGTAAAAAAC
  AAGGAGTCTCTGAAAATCGCAACAAAGCAAAAACCCAGGATGGAGTGGCCTTGGAAATCCAGCCACTCAA
  CAGCCAGGAGGGGCTCGATAGTGAGGAAAAGGAGAAGAAAGCAAGCAAGGGACCCAAGAAGGAGAAGTCG
  GTCCTGCAGGGCAAGCTCACACGCCTGGCTGTGCAGATCGGGAAGGCAGGTCTGATCATGTCTATCCTCA
  CAGTCCTCATCCTGATTCTATACTTCGTGGTTGACAATTTCGTGATACAGCGCCGGGCATGGCTTCCTGA
  GTGCACTCCTGTGTACATCCAGTATTTTGTCAAGTTCTTCATCATCGGAGTCACTGTACTGGTAGTGGCT
  GTGCCAGAGGGGCTGCCACTGGCTGTCACCATCTCGTTGGCCTACTCTGTAAAGAAAATGATGAAGGACA
  ACAACTTGGTCCGGCACTTGGACGCCTGTGAGACGATGGGCAACGCCACAGCCATCTGCTCTGATAAGAC
  GGGCACGCTGACCATGAACCGCATGACCGTGGTACAAGCTTACATTGGGGGCACTCACTACCGCCAGATC
  CCAAAACCTGACGACCTCCCACCCAACGTTCTGGACCTCATAGTCAACAGCATCTGTATCAACTCTGCTT
  ACACTTCTAAGATTCTGCCTCCAGAGAAAGAGGGAGGCCTCCCCCGGCAAGTAGGCAACAAGACAGAGTG
  TGGACTGCTGGGCTTTGTCACAGACCTGAAGCAGGACTACCAAGCAGTTCGCAGTGAGATGCCCGAGGAG
  AAGCTCTTCAAGGTGTACACCTTCAACTCAGTGCGCAAGTCCATGAGCACCGTCATCAGGAAGCCGGAAG
  GGGGCTTCCGCGTGTTCAGCAAGGGCGCCTCCGAGATAATGCTGCGCAAGTGTGATCGGATCCTGAACAA
  GGAAGGAGGAATTGTACCATTCAAAACTAAGGACCGAGACAATATGGTACGCAACGTCATTGAGCCCATG
  GCCAGTGAAGGACTCCGGACCATCGGCATAGCTTACCGAGATTTTGATGGTGAAGAGCCCTCGTGGGAAA
  ATGAGAATGAGATCTTCACTGGGCTGGTCTGCATTGCAGTGGTGGGCATTGAGGACCCTGTGCGCCCAGA
  GGTACCAGATGCCATTAACAAGTGCAAACGGGCTGGCATTACTGTCAGGATGGTGACAGGTGACAACGTC
  AACACAGCCAGGGCCATCGCCACCAAATGTGGCATCTTGACTCCTGGTGATGACTTCCTGTGTCTGGAAG
  GGAAAGAATTCAACAGACTCATACGCAATGAGAAGGGCGAGGTCGAACAAGAAAAGCTGGACAAGGTCTG
  GCCCAGGCTTCGGGTACTGGCACGATCTTCTCCCACAGACAAGCACACATTGGTGAAAGGTATCATTGAC
  AGCAATATTGGGGAACAGAGGCAGGTGGTGGCTGTCACCGGTGATGGAACCAATGATGGACCGGCTCTAA
  AGAAAGCAGATGTCGGTTTTGCTATGGGCATTGCAGGCACAGATGTGGCTAAGGAGGCATCAGACATCAT
  CCTGACTGATGACAACTTCACCAGCATTGTGAAGGCAGTGATGTGGGGCCGGAACGTATACGACAGCATT
  TCCAAGTTCCTGCAGTTCCAGCTCACTGTCAACGTGGTGGCCGTGATTGTGGCTTTCTCTGGAGCCTGCA
  TCACTCAGGATTCCCCACTGAAAGCGGTGCAGATGCTGTGGGTGAACCTGATCATGGACACGTTTGCTTC
  TCTGGCCCTGGCCACAGAGCCCCCGACCGATTCCCTGCTGAGGCGTCGCCCCTATGGGCGAAACAAGCCC
  CTGATCTCTCGCACAATGATGAAGAACATCCTGGGCCACGCGGTGTATCAGCTCGGAATCGTCTTCCTCC
  TCGTCTTTGCTGGCGACAAACTGTTTGACATTGACAGTGGGAGGAAGGCGCCTCTCAATTCACCACCCAG
  CCAGCACTACACCATTGTTTTCAACACGTTTGTGCTGATGCAACTCTTCAACGAGATCAACTCTCGGAAG
  ATCCATGGCGAGAAGAATGTCTTTGCAGGTGTCTACCGCAACATCATTTTCTGCTCTGTTGTTTTAGGCA
  CGTTCTTCTGCCAGATCTTGATCGTGGAAGTGGGAGGTAAGCCCTTCAGCTGCACAAACCTCACCATGGA
  GCAGTGGATGTGGTGTCTCTTTATAGGGATCGGAGAACTTTTGTGGGGCCAGGTCATCTCTGCAATACCT
  ACCAAGTCTCTAAAGTTCCTGAAAGAGGCGGGCCATGGCAGTGACAAAGAGGAGATCAGCAAGGACGCTG
  AGGGGCTGGAAGAGATCGACCATGCCGAGATGGAGCTTCGCCGAGGCCAGATCCTCTGGGTCCGTGGCCT
  GAACCGGATTCAGACTCAGATTGAAGTAATTAACAAATTCCAGACGGGAGCCTCTTTTAAGGGAGTCCTA
  AGGCGACAGAACTTGAGTCAACAACTTGATGTAAAACTGGTTCCTAGCTCCTATTCAGAAGCAGTTGCGT
  CAGTCAGAACCTCCCCCTCCACCTCTTCTGCCGTTACACCTCCTCCTGTGGGCAATCAGAGTGGTCAAAG
  TATTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202745 representing NM_001005871
  Red=Cloning site Green=Tags(s)
  
  MTNPSGHNLPANSVAESYEGEFGCTLMDLRKLMELRAADAVTQISAHYGSVQEICARLKTSPVEGLSGNP
  ADLEKRRLVFGKNMIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPPGGENEICGHIVSNPE
  EDEEGETGWIEGAAILASVIIVVFVTAFNDWSKEKQFRGLQSRIELEQKFSIIRNGQLIQLPVAEIVVGD
  IAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKTLDKDPMLLSGTHVMEGSGRMVVTAVGINSQT
  GIIFTLLGANEEEDDEKKKKGKKQGVSENRNKAKTQDGVALEIQPLNSQEGLDSEEKEKKASKGPKKEKS
  VLQGKLTRLAVQIGKAGLIMSILTVLILILYFVVDNFVIQRRAWLPECTPVYIQYFVKFFIIGVTVLVVA
  VPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGTHYRQI
  PKPDDLPPNVLDLIVNSICINSAYTSKILPPEKEGGLPRQVGNKTECGLLGFVTDLKQDYQAVRSEMPEE
  KLFKVYTFNSVRKSMSTVIRKPEGGFRVFSKGASEIMLRKCDRILNKEGGIVPFKTKDRDNMVRNVIEPM
  ASEGLRTIGIAYRDFDGEEPSWENENEIFTGLVCIAVVGIEDPVRPEVPDAINKCKRAGITVRMVTGDNV
  NTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKVWPRLRVLARSSPTDKHTLVKGIID
  SNIGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSI
  SKFLQFQLTVNVVAVIVAFSGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTDSLLRRRPYGRNKP
  LISRTMMKNILGHAVYQLGIVFLLVFAGDKLFDIDSGRKAPLNSPPSQHYTIVFNTFVLMQLFNEINSRK
  IHGEKNVFAGVYRNIIFCSVVLGTFFCQILIVEVGGKPFSCTNLTMEQWMWCLFIGIGELLWGQVISAIP
  TKSLKFLKEAGHGSDKEEISKDAEGLEEIDHAEMELRRGQILWVRGLNRIQTQIEVINKFQTGASFKGVL
  RRQNLSQQLDVKLVPSSYSEAVASVRTSPSTSSAVTPPPVGNQSGQSIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005871
• ORF Size: 3507 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005871.1, NP_001005871.1
• RefSeq Size: 4102 bp
• RefSeq ORF: 3510 bp
• Locus ID: 29600
• Cytogenetics: 13q13
• MW: 128.8 kDa
• Gene Summary: transports calcium; plays a role in regulation of calcium homeostasis and calcium-mediated signalling pathways [RGD, Feb 2006]