Syne4 (NM_001009283) Rat Tagged ORF Clone

CAT#: RR202866

Syne4 (Myc-DDK-tagged ORF) - Rat similar to Hypothetical protein MGC38513 (RGD1304580), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syne4
• Synonyms: KASH4; RGD1304580
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202866 representing NM_001009283
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGTTTCCACTTCTTGGCCATGGATTCCCTCCAGAGCCTGTGAATCACCCCCTGGGAGGTCCTA
  GGGGGCTGGACGTTGCCGGACCTACCATCTGCCCTGCACCTGAGGAGGAGCCGAGCAGGCCAGAGCAGGT
  CCAGGCCTCCTTGGACGCGCCTGAGCATTTCATGGATGAACCGAAGAGCACTGAGTCTGCCACCAGCCCC
  TCAAAACTACCCCTGGCCTCTTCCCATGAGCATCAAGATGGGGGCAAGCCCTGTGAGGCCCTGCAGGCAG
  AGCTCCAAGGGGCAGCCGAACGTGTGGATGCATTGCTTGTATTTGGTGAGGGCCTGGCAGAGAGGAGTGA
  GCCCAGAGCCTGGACATCCCTGGAGCAGGTCCTGAGGGCCCTTGGAACCCATCGAGACACCATCTTCCAA
  CGGCTCTGGCAGCTGCAGGCCCAGTTGATCAGCTATAGCCTGGTGCTTGAGAAGGCCAACCTACTGGACC
  AGGACTTGGAAGTTGAGGGAGACTCAGACGGGCCAGCAGCTGGTGGAGTCTGGGGGCCCTGGGCACCCAG
  TATCTTCCCCACTCCTGCAGAGTTGGAGTGGGACCCAGCAGGGGATGTTGGGGGTCTTGGACCCTCAGGG
  CAAAAGATATCTCGGATACCAGGAGCTCCCTGTGAGCTGTGTGGCTACAGGGGATCCCAGAGCAGTGGAC
  AGGGCTTTGAGGACCTGCTCTCACTGGGGCTTGGCCACCGGAAACATTTGGCGGCTCACCATCGAAGGCG
  TCTCCAGAAGCCTCAGGACAAGAAGAGGCAAGGACCTCCTAGTCTGCCCGATGCGATGCTGGAAGTGGAT
  CGTGGGGTCCCAGCTCCTGCATCCAGGCGGCCCCTGACCTTCCTCCTTCTCCTTCTCTTCCTCCTTCTGG
  TGGGTGCCACATTGTTGCTGCCCTTGTCGGGGGTCCCCTGCTGTTCTCATACCCGGCTGGCCAGGACGCC
  CTACCTGGTGCTCAGCTATGTCAATGGGCTCCCTCCAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202866 representing NM_001009283
  Red=Cloning site Green=Tags(s)
  
  MAQFPLLGHGFPPEPVNHPLGGPRGLDVAGPTICPAPEEEPSRPEQVQASLDAPEHFMDEPKSTESATSP
  SKLPLASSHEHQDGGKPCEALQAELQGAAERVDALLVFGEGLAERSEPRAWTSLEQVLRALGTHRDTIFQ
  RLWQLQAQLISYSLVLEKANLLDQDLEVEGDSDGPAAGGVWGPWAPSIFPTPAELEWDPAGDVGGLGPSG
  QKISRIPGAPCELCGYRGSQSSGQGFEDLLSLGLGHRKHLAAHHRRRLQKPQDKKRQGPPSLPDAMLEVD
  RGVPAPASRRPLTFLLLLLFLLLVGATLLLPLSGVPCCSHTRLARTPYLVLSYVNGLPPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009283
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009283.1, NP_001009283.1
• RefSeq Size: 1169 bp
• RefSeq ORF: 1023 bp
• Locus ID: 292781
• Cytogenetics: 1q21
• MW: 36.5 kDa