Cpt1c (NM_001034925) Rat Tagged ORF Clone

CAT#: RR202986

Cpt1c (Myc-DDK-tagged ORF) - Rat carnitine palmitoyltransferase 1c (Cpt1c), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cpt1c
• Synonyms: CPT1-B; CPTI-B; CPT IC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202986 representing NM_001034925
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGGCACACCAGGCCTCGAGCTTGCTGTCTTCACTCAGTTCCGACGGGGCAGAAGTGGAGCTCA
  GCTCCTCAGTGTGGCAGGAGATTTACCTCTCTGCCCTACGCTCCTGGAAAAGGAATCTCTGGCGTGTCTG
  GAATGACTTTCTTGCTGGTGTGGTCCCGGCAACGCCCCTCAGCTGGCTTTTCCTCTTCAGTACAATCCAG
  CTTGCTTGTCTCCTCCAGCTGGACCCTTCCCTAGGACTGATGGAGAAGATTAAAGAGTTGCTGCCAGACT
  GGGGCGGACAGCACCACCAGCTCCAGGGGCTCCTGGCAGCAGCAGTCTTTGCCTCGTGTTTGTGGGGAAC
  CCTGATCTTCACACTTCATGTGGCTCTGAGGCTCCTGCTGTCCCACCACGGCTGGCTTCTGGAACCTCAT
  GGCACCATGTCCTCACCCACGAAGACCTGGCTGGCATTGGTCAGAATCTTTTCGGGGAGGCACCCGAGGC
  TCTTCAGCTTTCAACGTGCGCTCCCGCGGCAGCCAGTGCCCGGAGCGCAAGAGACCGTGCGCAAGTACCT
  AGAGTCCATGCGCCCTGTGCTCCGAGACGACGCTTTCGACAGCGTTGTGGCGCTGGCAAACGACTTCCTG
  AGGTTGCAGGCGCCACGACTCCAGCTCTATTTGCAACTCAAGTCCTGGTGCGCTTCCAACTACGTCAGCG
  ACTGGTGGGAAGAGTTTGTGTACCTGCGTTCTCGAGGCTCGCTGGTTAATAGCACCTACTACATGATGGA
  TTTTCTGTATGTCACCCCCACACCACTGCAGGCAGCCCGTGCGGGCAATGCCGTGCATACCCTGCTCCTG
  TATCGCCACCTTCTGAACCGGCAGGAGATCCCACCGACCTTGCTGATGGGGATGCGCCCCTTGTGCTCCG
  CCCAGTACGAGAGGATGTTCAACACTACACGGATTCCAGGGGTGGAAAAAGACTACCTTTGTCACCTCCA
  AGACAGCCAACATGTGGCTGTCTTCCACCAGGGCAGATTCTTCCGTGTAGGGACCCACTCTTCCAATGGC
  CTGCTGTCCCCACGGGCCCTGGAACAGCAGTTCCAGTACATCCTGGATGACCCCTCCCCAGCCTGTCCCC
  TTGAGGAACATCTAGCTGCTTTGACTGCCGCTCCCAGGAGTATGTGGGCCCAGGTACGTGAGTCTGTGAA
  GACCCATGCGGCCACTGCCCTGGAGACTGTGGAAGGGGCTGCCTTCTTTGTGTCCCTGGATTCTGAGCCC
  GCGGGACTGACCAGAGAGAACCCCGCAGCTTCCCTGGACACCTATGCCCACACTCTGCTGACTGGCCAAG
  GCCATGACCGTTGGTTTGACAAATCCTTCACCCTCATCGTCTTCTCCAACGGGAAGCTGGGCCTCAGCGT
  GGAGCACTCATGGGCTGACTGCCCTGTCGCGGGACATTTATGGGAGTTCACCCTGGCCACAGAGTGCTTC
  CAGTTGGGATATGCCACAGATGGCCACTGTAAGGGGCACCCCGACCCTGCCCTGCCGAAGCCCCAGCGCC
  TGCAGTGGGACCTTCCAAAGCAGATCCAGCCGTCCATCTCTCTGGCCCTGAGGGGAGCCAAGACCTTGTC
  TGGCAATATTGACTGCCATGTCTTCCCCTTCTTCCACTTCGGCAAGAGCTTCATCAAAGGCTGTCATGTC
  TCTTCAGACAGTTTCATCCAGCTGGTCCTGCAGCTGGCCCACTTCCGGGACAGGGGTCAATTCTGCCTGA
  CTTATGAATCAGCCATGACCCGACTGTTTCTGGAAGGCAGGACGGAGACGGTGAGGTCTTGCACTAGAGA
  GGCCTGCCAATTTGTGAGAGCCATGGAAAACAAGGAGAGGACAGACCAACAGTGCCTTGCCCTGTTCCGG
  GAAGCCGTGGACAAGCACCAGGCCCTGCTGAAGGCAGCAATGAGTGGGCAAGGGATCGACCGTCACCTCT
  TCGCACTGTACATCATGTCCCGGCTTCTCCACATGCAGTCGCCCTTCCTGACCCAGGTCCAGTCGCAGCA
  ATGGCTTCTGTCTACCAGCCAGATCCCTGTGCAGCAGACACACCTGTTCGATGTCCACAATTACCCGGAT
  TATGTTTCCTCTGGAGGTGGATTTGGGCCTGCCCATGACCATGGGTATGGCGTCTCGTATATCTTCATGG
  GGGAAAACGCAATCAGCTTCCACATCTCCAGCAAGCAATCAAGCACAGAAACAGACTCACACCGGCTGGG
  GCAGCACATTGAAGATGCCCTGTTGGATGTAGCCTCCCTGTTCCAGGCTGGACAGCAATTCAAGCGCCAG
  TTCACAGGGCTCGGGGAGAGTTCAGGTTGGAAATACAGCAATCTCTCCTGTAAGACTGTGGACCCCAATA
  TCCCCAAATCCTCCACCAACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202986 representing NM_001034925
  Red=Cloning site Green=Tags(s)
  
  MAEAHQASSLLSSLSSDGAEVELSSSVWQEIYLSALRSWKRNLWRVWNDFLAGVVPATPLSWLFLFSTIQ
  LACLLQLDPSLGLMEKIKELLPDWGGQHHQLQGLLAAAVFASCLWGTLIFTLHVALRLLLSHHGWLLEPH
  GTMSSPTKTWLALVRIFSGRHPRLFSFQRALPRQPVPGAQETVRKYLESMRPVLRDDAFDSVVALANDFL
  RLQAPRLQLYLQLKSWCASNYVSDWWEEFVYLRSRGSLVNSTYYMMDFLYVTPTPLQAARAGNAVHTLLL
  YRHLLNRQEIPPTLLMGMRPLCSAQYERMFNTTRIPGVEKDYLCHLQDSQHVAVFHQGRFFRVGTHSSNG
  LLSPRALEQQFQYILDDPSPACPLEEHLAALTAAPRSMWAQVRESVKTHAATALETVEGAAFFVSLDSEP
  AGLTRENPAASLDTYAHTLLTGQGHDRWFDKSFTLIVFSNGKLGLSVEHSWADCPVAGHLWEFTLATECF
  QLGYATDGHCKGHPDPALPKPQRLQWDLPKQIQPSISLALRGAKTLSGNIDCHVFPFFHFGKSFIKGCHV
  SSDSFIQLVLQLAHFRDRGQFCLTYESAMTRLFLEGRTETVRSCTREACQFVRAMENKERTDQQCLALFR
  EAVDKHQALLKAAMSGQGIDRHLFALYIMSRLLHMQSPFLTQVQSQQWLLSTSQIPVQQTHLFDVHNYPD
  YVSSGGGFGPAHDHGYGVSYIFMGENAISFHISSKQSSTETDSHRLGQHIEDALLDVASLFQAGQQFKRQ
  FTGLGESSGWKYSNLSCKTVDPNIPKSSTNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034925
• ORF Size: 2403 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034925.2, NP_001030097.2
• RefSeq Size: 2747 bp
• RefSeq ORF: 2406 bp
• Locus ID: 308579
• Cytogenetics: 1q22
• MW: 90.2 kDa