Cacna1i (NM_020084) Rat Tagged ORF Clone

CAT#: RR203004

Cacna1i (Myc-DDK-tagged ORF) - Rat calcium channel, voltage-dependent, T type, alpha 1I subunit (Cacna1i), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1i
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203004 representing NM_020084
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACAGCAACTTACCGCCCTCATCTGCAGCAGCCCCGGCCCCTGAGCCGGGAATCACTGAGCAGC
  CGGGGCCCCGGAGTCCCCCTCCATCCCCTCCAGGCCTGGAGGAGCCATTGGAAGGAACCAACCCTGACGT
  CCCACATCCAGACCTGGCTCCTGTTGCTTTCTTCTGCCTGCGCCAGACCACGAGCCCACGGAACTGGTGC
  ATCAAGATGGTTTGTAACCCGTGGTTCGAGTGTGTGAGCATGCTGGTTATTCTGCTGAACTGTGTGACCC
  TGGGCATGTACCAGCCATGTGATGACATGGAGTGCCTGTCGGACCGTTGCAAGATCCTGCAGGTCTTCGA
  TGACTTCATCTTCATCTTCTTTGCCATGGAGATGGTGCTTAAGATGGTGGCCCTGGGCATTTTTGGCAAG
  AAGTGCTACCTCGGAGACACATGGAACCGCCTGGATTTCTTCATTGTCATGGCAGGGATGGTTGAGTACT
  CTCTGGACCTACAGAACATCAACCTGTCAGCCATCCGCACTGTGCGTGTCCTGAGGCCTCTCAAAGCCAT
  CAACCGTGTACCCAGCATGCGGATCCTGGTGAACCTGCTGCTCGACACGCTGCCCATGCTGGGGAACGTG
  CTCCTGCTCTGTTTCTTCGTCTTCTTCATCTTCGGCATCATTGGCGTGCAGCTCTGGGCAGGCCTGCTAC
  GGAACCGCTGCTTCCTGGAAGAGAACTTCACCATACAAGGGGATGTGGCCCTGCCCCCTTATTACCAACC
  AGAGGAGGATGACGAGATGCCCTTTATCTGCTCCCTGACTGGGGACAATGGCATCATGGGCTGCCACGAG
  ATCCCCCCACTGAAGGAGCAGGGCCGGGAATGCTGCCTGTCCAAAGATGATGTGTATGACTTCGGGGCGG
  GGCGCCAGGACCTCAACGCCAGCGGTCTGTGCGTCAACTGGAACCGCTACTACAACGTCTGCCGCACGGG
  CAACGCCAACCCTCACAAGGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATTGTGATTTTCCAG
  GTGATCACTCTGGAAGGCTGGGTGGAGATCATGTACTATGTGATGGACGCACATTCTTTCTACAACTTCA
  TCTACTTCATTCTGCTCATCATAGTGGGCTCCTTCTTCATGATCAACTTGTGCCTCGTTGTCATAGCAAC
  CCAGTTCTCTGAGACCAAGCAACGGGAGCACCGGCTGATGCTGGAGCAACGCCAGCGCTACCTGTCCTCC
  AGCACGGTGGCCAGTTACGCTGAGCCCGGTGATTGCTATGAGGAGATCTTCCAATATGTCTGTCACATCC
  TTCGCAAAGCCAAGCGCCGTGCCCTAGGCCTCTACCAGGCCCTGCAGAACCGGCGCCAGGCCATGGGCCC
  GGGGACACCAGCCCCTGCCAAGCCTGGGCCCCATGCCAAGGAGCCCAGCCACTGCAAGCTGTGCCCACGA
  CACAGCCCCCTGGACCCCACTCCCCACACACTGGTGCAGCCCATCTCTGCCATTCTGGCCTCTGACCCCA
  GCAGCTGCCCTCACTGCCAGCACGAGGCAGGCAGGCGGCCCTCTGGCCTGGGCAGCACTGACTCAGGCCA
  GGAAGGCTCAGGTTCTGGTGGCTCTGCAGAGGCCGAAGCCAATGGGGATGGACCCCAGAGCAGTGAGGAT
  GGGGTCTCCTCGGACCTGGGGAAGGAGGAGGAACAGGAGGACGGGGCAGCCCGACTGTGTGGGGATGTGT
  GGCGCGAGACACGAGAAAAGCTGCGGGGCATCGTGGACAGCAAGTACTTCAACAGAGGTATCATGATGGC
  TATCCTGGTGAACACAGTCAGCATGGGCATCGAGCACCACGAACAGCCCGAGGAGCTGACCAACATCCTG
  GAGATCTGCAATGTGGTCTTCACCAGTATGTTTGCCCTGGAGATGATCCTGAAACTGGCCGCCTTTGGGC
  TCTTCGACTACCTGCGGAACCCTTACAACATCTTTGACAGCATCATCGTCATCATCAGCATCTGGGAAAT
  CGTGGGGCAGGCAGACGGTGGCCTGTCTGTGCTGCGCACCTTCCGGTTGCTGCGGGTGCTGAAGCTGGTG
  CGCTTCATGCCGGCGCTGCGGCGCCAGCTCGTGGTGCTCATGAAGACCATGGACAACGTGGCCACCTTCT
  GCATGCTACTCATGCTGTTCATCTTCATCTTCAGCATCCTTGGGATGCATATCTTTGGCTGCAAATTCAG
  CCTCCGCACGGACACGGGAGACACCGTTCCTGACAGGAAGAACTTCGATTCCTTACTGTGGGCCATCGTC
  ACAGTGTTCCAGATCCTCACTCAGGAGGACTGGAACGTTGTCCTGTACAATGGCATGGCCTCCACCACCC
  CCTGGGCCTCCCTCTATTTTGTTGCCCTCATGACCTTTGGCAACTACGTTCTCTTCAATCTCCTGGTGGC
  TATCCTGGTAGAGGGTTTCCAGGCTGAGGGTGATGCTAATCGTTCCTACTCTGATGAGGACCAGAGCTCA
  TCCAATTTGGAGGAGTTTGACAAGCTCCCAGAGGGCCTGGACAACAGTAGAGATCTCAAGCTCTGCCCAA
  TACCCATGACACCCAATGGACACCTGGACCCTAGCCTCCCTCTGGGTGCGCATCTGGGTCCTGCTGGTAC
  CATGGGTACTGCCCCCCGCCTCTCACTGCAGCCAGACCCGGTACTGGTGGCCCTAGACTCTCGGAAAAGC
  AGTGTCATGTCCCTGGGCAGGATGAGCTATGATCAGCGATCCTTGTCCAGCTCCCGGAGCTCCTACTACG
  GGCCCTGGGGCCGCAGTGGGACCTGGGCTAGCCGCCGCTCCAGCTGGAACAGCCTGAAACACAAGCCGCC
  CTCAGCTGAGCATGAGTCCTTACTGTCTGGGGAGGGTGGAGGTAGCTGCGTCAGGGCCTGTGAAGGCGCC
  CGGGAGGAGGCGCCAACTCGCACCGCACCCCTGCATGCTCCACACGCGCACCACGCGCACCATGGACCCC
  ACCTGGCACACCGTCACCGACACCACCGCCGGACTCTGTCCCTTGATACCAGGGACTCTGTTGACCTGGG
  AGAGCTGGTGCCCGTGGTGGGTGCCCACTCACGGGCCGCTTGGAGGGGGGCGGGTCAGGCCCCTGGGCAC
  GAGGACTGCAATGGCAGAATGCCCAACATAGCCAAGGATGTCTTCACCAAGATGGATGACTGCCGCGACC
  GCGGGGAGGACGAGGAGGAGATCGACTATACCCTGTGTTTCCGGGTCCGCAAGATGATTGATGTGTACAA
  GCCGGACTGGTGCGAAGTCCGCGAGGACTGGTCGGTCTACCTCTTCTCCCCCGAGAACAAGTTCCGGATC
  CTGTGTCAGACTATCATTGCTCACAAGCTTTTTGACTACGTGGTCTTGGCCTTTATCTTCCTCAACTGTA
  TCACCATTGCTCTGGAGAGACCCCAGATTGAAGCTGGTAGCACTGAGCGCATCTTCCTCACGGTGTCTAA
  CTACATCTTCACAGCCATCTTCGTGGGCGAGATGACACTGAAGGTGGTTTCTCTGGGCCTGTACTTTGGT
  GAGCAGGCGTACCTGCGTAGCAGCTGGAATGTACTGGATGGTTTCCTGGTCTTTGTGTCCATCATCGATA
  TCGTAGTGTCCGTGGCCTCTGCTGGGGGAGCCAAGATTCTGGGGGTCCTCCGGGTCCTGCGGCTCCTGCG
  TACCTTACGTCCTTTGAGGGTTATCAGCCGGGCCCCTGGGCTGAAGCTGGTGGTAGAGACGCTCATCTCC
  TCCCTCAAGCCCATTGGGAACATCGTCCTCATCTGCTGTGCCTTCTTCATCATCTTCGGCATCCTGGGGG
  TGCAGCTTTTCAAAGGCAAGTTCTACCATTGTTTGGGAGTGGACACCCGAAACATCACCAACCGATCTGA
  CTGCGTGGCGGCCAACTACCGCTGGGTGCATCACAAATACAACTTTGACAACCTGGGCCAGGCATTGATG
  TCCCTCTTTGTCTTGGCCTCCAAGGACGGCTGGGTGAACATCATGTATAATGGATTAGATGCTGTTGCTG
  TGGACCAGCAGCCAGTGACGAACCACAACCCCTGGATGCTACTGTACTTCATTTCGTTCCTGCTCATCGT
  CAGCTTCTTTGTGCTCAACATGTTTGTGGGCGTGGTCGTGGAGAACTTCCACAAGTGCCGGCAGCACCAG
  GAGGCTGAGGAGGCGCGGAGGCGTGAGGAGAAACGGCTGCGGCGCCTGGAAAAGAAGCGCCGTAAGGCTC
  AGAGGCTGCCCTACTATGCTACCTACTGTCCCACAAGGCTGCTCATCCACTCCATGTGCACCAGCCACTA
  CCTGGACATCTTCATTACCTTCATCATCTGCCTCAATGTTGTCACCATGTCCCTGGAGCACTACAACCAG
  CCTACATCCCTAGAGACAGCCCTTAAGTACTGCAACTACATGTTCACCACTGTCTTTGTGCTGGAGGCTG
  TGCTGAAGCTGGTGGCATTTGGCCTGAGGCGTTTCTTCAAGGACCGATGGAACCAGCTGGACCTGGCCAT
  TGTGCTGCTGTCCGTCATGGGCATCACACTGGAGGAGATCGAGATCAATGCCGCCCTTCCCATCAACCCC
  ACCATCATCCGTATCATGCGTGTTCTGCGTATCGCCCGGGTGTTGAAGCTATTGAAGATGGCCACAGGAA
  TGCGGGCCCTGCTGGACACAGTGGTACAGGCTCTGCCCCAGGTGGGCAACCTGGGCCTGCTCTTCATGCT
  GCTCTTCTTCATCTATGCTGCTCTGGGAGTGGAGCTCTTCGGAAAGCTGGTCTGCAATGACGAGAACCCG
  TGTGAGGGCATGAGCCGGCACGCCACCTTTGAAAACTTCGGCATGGCCTTCCTCACGCTCTTCCAGGTCT
  CCACAGGCGATAACTGGAATGGAATTATGAAGGACACCCTGCGAGACTGTACCCATGATGAGCGCAGCTG
  CCTAAGCAGCCTGCAGTTTGTGTCACCGCTCTACTTTGTGAGCTTCGTGCTCACAGCTCAGTTCGTGCTC
  ATCAACGTGGTGGTGGCCGTGCTGATGAAACATCTGGATGACAGCAACAAGGAGGCCCAGGAGGATGCAG
  AGATGGATGCTGAGATCGAGCTGGAGATGGCCCATGGCCTCGGCCCCTGCCCTGGCCCCTGCCCTGGTCC
  CTGCCCCTGCCCCTGGCCCCTAGCCCCTGTCCTGGCCCGAGGCTGCCCGCTAGTTCTCCTAGGGGCTACC
  GGGGCGAGGATCGGGAGGGGCAGGTGCTGGAGGCGACACCGAGAGTCACCTGTGCCGAGCACTCGCTATT
  CTCCAGCCCAGGAGACCCTGTGGCTGGACAGCGTCTCTTTAATCATCAAGGACTCCTTGGAGGGGGAGCT
  GACCATCATTGACAACCTGTCTGGGTCCGTCTTCCACCACTACGCCTCACCTGACGGCTGTGGCAAGTGT
  CACCATGACAAGCAAGAGGTGCAGCTGGCTGAGACAGAGGCCTTCTCCCTGAACTCAGACAGGTCTTCAT
  CCATCCTGCTGGGGGATGACCTGAGTCTTGAGGACCCCACGGCCTGCCCACCTGGCCGCAAAGACAGCAA
  GGGTGAGCTGGACCCACCTGAGCCCATGCGTGTGGGAGACCTGGGCGAATGCTTCTTCCCCTTGTCCTCT
  ACGGCCGTCTCGCCGGATCCAGAGAACTTCCTGTGTGAGATGGAGGAGATCCCATTCAACCCTGTCCAGT
  CCTGGCTCAAACATGACAGCAGTCAAGCACCCCCGAGCCCTTTCTCCCCGGATGGCTCCAGCCCTCTCCT
  GCAGATGCCTGCTGAGTTCTTCCACCCTGCTGTGTCTGCCAGCCAGAAAGGCCCAGAAAAGGGCACTGGC
  ACTGGAACCCTGCCCAAGATTGCACTTCAGGGGTCCTGGGCATCGCTGAGGTCACCGAGTGTCAACTGCA
  CCCTCCTCCGGCAGGCCACCGGGAGCGACACGTCGCTGGACGCCAGCCCCAGCAGCTCAGCGGGCAGCCT
  ACAGACCACACTGGAAGACAGTCTGACTCTGAGTGACAGTCCCCGGCGTGCCCTGGGGCCGCCGGTCCAG
  GTGCCTGGGCCACGGGCTAGCCTGTCACCGGCCACCCGGCGCCGCCTCAGCCTGCGGGGCCGTGGCCTGT
  TTAGTCTGCGTGGGCTGCGGGCCCATCAGCGTAGCCACAGCAGTGGCGGCTCCACCAGCCCTGGCTGCAC
  TCACCACGACTCCATGGACCCCTCTGATGAGGAGGGCCGCGGGGGCGCAGGTGGCGGGGGTGCAGGCAGT
  GAGCACTCGGAGACCCTCAGCAGCCTGTCACTCACATCTCTCTTCTGCCTACCACCCACGCTGCCACCAC
  CCGGCCTCACCCCAGCCAGGAAGTTCAACAGTACCAGCAGCCTGGCCGCTGGACCTGGCCGTCCTGGCTC
  CACTGTCTCAGCCCGTGGTCTGGTCAGAAGCCCCTCATGGGCTGCAGACCGCAGCAAGGACCCACCAGGC
  CAAGCTCAGCTGGTCTCAGGCTTGGGCTCCTCAGCACCCGGGCCACAGCCACCTCCCGGGGAGTCAACTG
  ATGCTGCGAGCAAAAGGAAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203004 representing NM_020084
  Red=Cloning site Green=Tags(s)
  
  MADSNLPPSSAAAPAPEPGITEQPGPRSPPPSPPGLEEPLEGTNPDVPHPDLAPVAFFCLRQTTSPRNWC
  IKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMECLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGK
  KCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNV
  LLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLTGDNGIMGCHE
  IPPLKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGNANPHKGAINFDNIGYAWIVIFQ
  VITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSS
  STVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQNRRQAMGPGTPAPAKPGPHAKEPSHCKLCPR
  HSPLDPTPHTLVQPISAILASDPSSCPHCQHEAGRRPSGLGSTDSGQEGSGSGGSAEAEANGDGPQSSED
  GVSSDLGKEEEQEDGAARLCGDVWRETREKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNIL
  EICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLV
  RFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIV
  TVFQILTQEDWNVVLYNGMASTTPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSS
  SNLEEFDKLPEGLDNSRDLKLCPIPMTPNGHLDPSLPLGAHLGPAGTMGTAPRLSLQPDPVLVALDSRKS
  SVMSLGRMSYDQRSLSSSRSSYYGPWGRSGTWASRRSSWNSLKHKPPSAEHESLLSGEGGGSCVRACEGA
  REEAPTRTAPLHAPHAHHAHHGPHLAHRHRHHRRTLSLDTRDSVDLGELVPVVGAHSRAAWRGAGQAPGH
  EDCNGRMPNIAKDVFTKMDDCRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENKFRI
  LCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFG
  EQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLIS
  SLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCVAANYRWVHHKYNFDNLGQALM
  SLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ
  EAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQ
  PTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINP
  TIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENP
  CEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVL
  INVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLGPCPGPCPGPCPCPWPLAPVLARGCPLVLLGAT
  GARIGRGRCWRRHRESPVPSTRYSPAQETLWLDSVSLIIKDSLEGELTIIDNLSGSVFHHYASPDGCGKC
  HHDKQEVQLAETEAFSLNSDRSSSILLGDDLSLEDPTACPPGRKDSKGELDPPEPMRVGDLGECFFPLSS
  TAVSPDPENFLCEMEEIPFNPVQSWLKHDSSQAPPSPFSPDGSSPLLQMPAEFFHPAVSASQKGPEKGTG
  TGTLPKIALQGSWASLRSPSVNCTLLRQATGSDTSLDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPVQ
  VPGPRASLSPATRRRLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTHHDSMDPSDEEGRGGAGGGGAGS
  EHSETLSSLSLTSLFCLPPTLPPPGLTPARKFNSTSSLAAGPGRPGSTVSARGLVRSPSWAADRSKDPPG
  QAQLVSGLGSSAPGPQPPPGESTDAASKRKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020084
• ORF Size: 6603 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020084.3, NP_064469.3
• RefSeq Size: 6709
• RefSeq ORF: 6606
• Locus ID: 56827
• MW: 243.7 kDa
• Gene Summary: low voltage-activated calcium channel subunit; important for calcium ion transport into nerve, muscle and endocrine cells [RGD, Feb 2006]