Wt1 (NM_031534) Rat Tagged ORF Clone

CAT#: RR203084

  • TrueORF®

Wt1 (Myc-DDK-tagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug)


  "NM_031534" in other vectors (5)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Wt1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR203084 representing NM_031534
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTTCCGACGTGCGGGACCTGAACGCGCTGCTGCCCGCCGTGTCCTCGCTGGGCGGTGGAGGCGGCT
GTGGGCTCCCTGTGAGCGGCGCGGCGCAATGGGCGCCGGTGCTGGACTTCGCGCCTCCGGGCGCCTCGGC
TTACGGGTCACTGGGCGGTCCCGCGCCTCCTCCCGCTCCGCCGCCACCTCCGCCGCCACCCCACTCCTTC
ATCAAACAGGAGCCCAGCTGGGGCGGCGCCGAGCCGCACGAGGAGCAGTGCCTGAGCGCCTTCACCTTGC
ACTTCTCCGGCCAGTTCACCGGTACAGCCGGGGCCTGTCGCTACGGACCCTTCGGTCCTCCCCCGCCCAG
CCAGGCGTCATCCGGCCAGGCCAGGATGTTCCCCAATGCGCCCTACCTGCCCAGCTGCCTGGAGAGCCAG
CCCTCCATCCGCAACCAAGGATACAGCACGGTCACTTTCGACGGGGCACCCAGTTATGGCCACACGCCCT
CGCACCATGCAGCGCAGTTCCCCAATCATTCCTTCAAACACGAGGACCCCATGGGCCAGCAGGGCTCGCT
GGGCGAGCAGCAGTACTCGGTGCCACCTCCAGTGTATGGCTGCCACACCCCTACCGACAGTTGCACAGGC
AGCCAGGCCCTGCTCCTGAGGACGCCCTACAGCAGTGACAATTTATACCAGATGACCTCCCAGCTTGAGT
GCATGACCTGGAACCAGATGAACCTCGGAGCTACCTTGAAGGGAATGGCTGCTGGGAGCTCCAGCTCAGT
GAAATGGACAGAAGGGCAGAGCAACCACGGCACAGGGTACGAGAGTGAGAACCACACAACCCCCATCCTC
TGTGGTGCCCAGTACAGAATACACACCCACGGTGTCTTCCGAGGCATTCAGGATGTGCGGCGCGTATCTG
GAGTGGCCCCAACTCTTGTCCGGTCAGCATCTGAAACCAGTGAGAAGCGTCCTTTCATGTGTGCGTACCC
AGGCTGCAATAAGAGATATTTTAAGCTGTCCCACTTACAGATGCACAGCCGGAAGCACACTGGTGAGAAA
CCATACCAGTGTGACTTCAAGGACTGCGAGAGAAGGTTTTCTCGCTCAGACCAGCTCAAAAGGCACCAAA
GGAGACACACAGGTGTGAAACCATTCCAGTGTAAAACTTGTCAGCGAAAGTTTTCCCGGTCCGACCACCT
GAAGACCCACACCAGGACTCATACAGGTAAAACAAGTGAAAAGCCCTTCAGCTGTCGGTGGCACAGTTGT
CAGAAAAAGTTTGCGCGGTCAGACGAATTAGTCCGCCATCACAACATGCACCAGAGAAACATGACCAAAC
TCCAGCTGGCGCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR203084 representing NM_031534
Red=Cloning site Green=Tags(s)

MGSDVRDLNALLPAVSSLGGGGGCGLPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPHSF
IKQEPSWGGAEPHEEQCLSAFTLHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQ
PSIRNQGYSTVTFDGAPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTG
SQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGMAAGSSSSVKWTEGQSNHGTGYESENHTTPIL
CGAQYRIHTHGVFRGIQDVRRVSGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEK
PYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWHSC
QKKFARSDELVRHHNMHQRNMTKLQLAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031534
ORF Size 1344 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_031534.1, NM_031534.2, NP_113722.2
RefSeq Size 2645
RefSeq ORF 1347
Locus ID 24883
MW 49.1 kDa
Gene Summary This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the human gene is mutated in a small subset of patients with Wilm's tumors. Authors of PMID:7926762 provide evidence that Wt1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Jul 2008]

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