Chaf1b (NM_001024741) Rat Tagged ORF Clone

CAT#: RR203091

Chaf1b (Myc-DDK-tagged ORF) - Rat chromatin assembly factor 1, subunit B (p60) (Chaf1b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chaf1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203091 representing NM_001024741
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTGATCACGTGTGAAATCGCCTGGCACAACAAGGAGCCAGTGTACAGCCTTGACTTCCAGCATG
  CGGCAGCCTGTAGGATCCAAAGGCTGGCGTCCGCGGGGGTGGACACCGCCGTTAGGATCTGGAAGGTGGA
  GAAGGGGCCAGATGGAAAAGCCATTGTGGAATTCCTCTCCAACCTTGCTCGGCACACCAAAGCTGTCAAT
  GTTGTACGCTTTTCTCCAACTGGGGAGATTTTAGCATCTGGAGGAGACGATGCCGTCATCCTGCTGTGGA
  AGATGAATGACAGCAAGGAGCCGGAGCAGATCGCCTTTCAGGATGAAGATGAGGCTCAGCTGAACAAGGA
  GAACTGGACTGTGGTAAAAACCCTGAGGGGCCACTTAGAAGATGTGTATGATATATGCTGGGCAACTGAT
  GGGAATATGATGGCCTCTGCTTCTGTGGATAACACAGTTATCATATGGGATGTCAGTAAAGGACAGAAGA
  TTTCCATTTTCAACGAGCATAAAAGTTACGTGCAAGGAGTGACCTGGGATCCTCTGGGTCAATATATCGC
  GACCCTGAGTTGTGACAGGGTGCTGCGGGTCTATAGCACCCAGAAGAAACGTGTGGCTTTCAACGTTTCA
  AAGATGCTCTCTGGACAAGGGCCCGAAGGAGAGGCGAGAAGTTTCCGGATGTTTCACGATGACAGCATGA
  AGTCGTTCTTCCGAAGACTCAGTTTTACTCCAGATGGATCTTTGCTCCTCACTCCAGCTGGATGTATGGA
  GTCTGGTGAGAATGTGACAAACACCACTTATGTGTTCTCCAGAGAACATCTGAAAAGGCCGATTGCCCAC
  CTTCCATGCCCTGGGAAAGCGACCCTTGCTGTCCGATGCTGTCCTGTGTACTTTGAACTGAGGCCAGTGG
  CAGAAACAGAGAAAGCGTCAGAGGAGCCCAGCCCCGAGCTGGTGAGTCTGCCCTACCGTATGGTGTTCGC
  AGTGGCCTCGGAGGACTCTGTGCTGCTGTATGACACGCAACAGTCTTTCCCATTTGGTTATGTGTCTAAC
  ATCCATTACCATACCCTGAGTGACATTTCCTGGTCCAGCGATGGGGCCTTCCTGGCTGTGTCCTCCACGG
  ATGGTTATTGCTCATTCGTGACCTTTGAGAAAGGTGAACTAGGCGTGCCTTTGAAGGAGAAGCCGGTTCT
  GGGCGTAAGAACTCCTGACACAGCAAAGAAAACCAAGAACCAGAACCCCCAGGGATCTTCGCCAGGATCC
  AGATCAGCCGAAGGAACCCCCAGCAACAGAATCCAGGACCCCAGCAGCCCCTGTACCACCCCCTCACCAA
  CCACACAGTCTCCAGCCCCGCCCGCCATCAAGGACAGTCCCTCAGCCATCCCTGCCGGCAAAAGCCCCTT
  GCCACGGCCTTCTGAGGAGAAGACCCTGCAGCCTGCCGCTCAAAACATGAAAGCCCCCCAACCCCGCAGG
  GTCACACTGAACACGCTGCAGTCTTGGGGCAAGACGGCCCCCCGGAGAATAAATTTAACACCGTTGAGGG
  CAGACACTGCACCAAATCCACTGCCCAGTAGCGGGGCCGCCCCCTCCACAGAAGAGGTCCAGCCAGAGGT
  GCCTGGAGAGCCCCAGGATGAGCCTCCCGAACTGAAGAGACCTAAGCTTGAAGAACAGGAAGGAGACACC
  CAAAGCCTGAGTCCTGATGAGACTTCCAAGTCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203091 representing NM_001024741
  Red=Cloning site Green=Tags(s)
  
  MKVITCEIAWHNKEPVYSLDFQHAAACRIQRLASAGVDTAVRIWKVEKGPDGKAIVEFLSNLARHTKAVN
  VVRFSPTGEILASGGDDAVILLWKMNDSKEPEQIAFQDEDEAQLNKENWTVVKTLRGHLEDVYDICWATD
  GNMMASASVDNTVIIWDVSKGQKISIFNEHKSYVQGVTWDPLGQYIATLSCDRVLRVYSTQKKRVAFNVS
  KMLSGQGPEGEARSFRMFHDDSMKSFFRRLSFTPDGSLLLTPAGCMESGENVTNTTYVFSREHLKRPIAH
  LPCPGKATLAVRCCPVYFELRPVAETEKASEEPSPELVSLPYRMVFAVASEDSVLLYDTQQSFPFGYVSN
  IHYHTLSDISWSSDGAFLAVSSTDGYCSFVTFEKGELGVPLKEKPVLGVRTPDTAKKTKNQNPQGSSPGS
  RSAEGTPSNRIQDPSSPCTTPSPTTQSPAPPAIKDSPSAIPAGKSPLPRPSEEKTLQPAAQNMKAPQPRR
  VTLNTLQSWGKTAPRRINLTPLRADTAPNPLPSSGAAPSTEEVQPEVPGEPQDEPPELKRPKLEEQEGDT
  QSLSPDETSKSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024741
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024741.1, NP_001019912.1
• RefSeq Size: 1958 bp
• RefSeq ORF: 1719 bp
• Locus ID: 288242
• Cytogenetics: 11q11
• MW: 62.7 kDa