Mapk10 (NM_012806) Rat Tagged ORF Clone

CAT#: RR203224

Mapk10 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase 10 (Mapk10), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mapk10
• Synonyms: Jnk3; SAPb; SAPKC; Serk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203224 representing NM_012806
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAAAGCAAGGTAGATAACCAGTTCTACAGTGTGGAAGTGGGAGACTCAACCTTCACAGTTCTAA
  AGCGCTACCAGAACCTGAAGCCGATCGGCTCTGGGGCTCAGGGAATAGTTTGTGCTGCGTATGACGCTGT
  CCTCGACAGAAATGTGGCCATTAAGAAGCTCAGCAGACCCTTCCAGAACCAAACTCATGCCAAGAGGGCT
  TACCGGGAGCTGGTCCTCATGAAGTGTGTGAACCATAAAAACATTATTAGCTTATTAAATGTCTTTACAC
  CCCAGAAAACACTGGAGGAGTTCCAAGATGTTTACTTAGTGATGGAACTGATGGACGCCAACTTGTGTCA
  GGTGATTCAGATGGAGCTGGACCACGAGCGGATGTCGTACTTGCTGTACCAGATGCTGTCGGCGATCAAA
  CACCTCCACTCCGCTGGGATCATCCACAGGGACTTAAAACCCAGTAACATCGTAGTCAAGTCTGATTGCA
  CACTGAAAATCCTGGACTTTGGACTGGCCAGGACAGCGGGCACAAGCTTCATGATGACTCCGTATGTGGT
  GACGAGATATTACAGAGCCCCCGAGGTCATCCTGGGCATGGGCTACAAGGAGAACGTGGACATATGGTCT
  GTGGGCTGCATCATGGGAGAAATGGTTCGTCACAAAATCCTCTTTCCCGGAAGGGACTATATTGACCAGT
  GGAACAAAGTCATAGAGCAGCTAGGAACTCCGTGTCCAGAATTCATGAAGAAATTGCAGCCCACCGTCAG
  AAACTACGTGGAGAACCGGCCCAAGTATGCAGGCCTCACCTTCCCCAAGCTCTTTCCAGATTCCCTCTTC
  CCAGCGGATTCCGAGCACAATAAACTTAAAGCCAGCCAAGCCAGGGACTTGTTGTCAAAGATGTTAGTGA
  TTGACCCAGCGAAGAGGATATCGGTGGATGACGCATTGCAGCATCCGTACATCAACGTTTGGTACGACCC
  TGCTGAAGTGGAGGCGCCTCCGCCTCAGATATATGACAAGCAATTGGATGAAAGGGAGCACACCATCGAA
  GAATGGAAAGAACTCATCTACAAGGAAGTAATGAACTCAGAAGAGAAGACTAAGAACGGCGTAGTCAAAG
  GCCAGCCCTCACCTTCAGGTGCAGCAGTGAACAGCAGTGAGAGTCTCCCTCCATCCTCATCTGTCAACGA
  CATCTCCTCCATGTCCACCGACCAGACCCTCGCATCCGACACTGACAGCAGCCTGGAAGCCTCGGCGGGA
  CCGCTGGGTTGTTGCAGGCCGCTGGGTTGTTGCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203224 representing NM_012806
  Red=Cloning site Green=Tags(s)
  
  MSKSKVDNQFYSVEVGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAVLDRNVAIKKLSRPFQNQTHAKRA
  YRELVLMKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIQMELDHERMSYLLYQMLSAIK
  HLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDIWS
  VGCIMGEMVRHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLF
  PADSEHNKLKASQARDLLSKMLVIDPAKRISVDDALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHTIE
  EWKELIYKEVMNSEEKTKNGVVKGQPSPSGAAVNSSESLPPSSSVNDISSMSTDQTLASDTDSSLEASAG
  PLGCCRPLGCCR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012806
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq Size: 2786
• RefSeq ORF: 1281
• Locus ID: 25272
• MW: 48.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]