Syt1 (NM_001033680) Rat Tagged ORF Clone

CAT#: RR203399

  • TrueORF®

Syt1 (Myc-DDK-tagged ORF) - Rat synaptotagmin I (Syt1), (10 ug)


  "NM_001033680" in other vectors (3)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Syt1
Synonyms P65
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR203399 representing NM_001033680
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAGTGCCAGTCATCCTGAGGCCCTGGCCGCCCCTGTCACCACTGTTGCGACCCTTGTCCCACACA
ATGCCACTGAGCCAGCCAGTCCTGGGGAAGGGAAGGAAGATGCCTTTTCCAAGCTGAAGCAGAAGTTTAT
GAATGAGCTGCATAAAATTCCATTGCCACCGTGGGCCTTAATAGCCATAGCCATAGTTGCGGTCCTTTTA
GTCGTAACCTGCTGCTTTTGTGTCTGTAAGAAATGTTTGTTCAAAAAGAAAAACAAGAAGAAGGGGAAGG
AAAAGGGAGGAAAGAACGCCATTAACATGAAAGACGTGAAAGACTTAGGGAAGACCATGAAGGATCAGGC
CCTTAAGGATGACGATGCTGAAACCGGACTGACTGATGGAGAAGAAAAGGAAGAGCCCAAGGAAGAGGAG
AAACTGGGAAAGCTCCAATATTCACTGGACTATGACTTCCAGAATAACCAGCTGTTGGTGGGAATCATCC
AGGCTGCTGAACTGCCCGCCCTGGACATGGGGGGTACATCCGATCCATACGTCAAAGTCTTCCTGCTGCC
TGACAAAAAGAAGAAATTTGAGACTAAAGTCCACCGGAAAACCCTCAATCCAGTCTTCAATGAACAATTT
ACTTTCAAGGTGCCCTACTCGGAATTAGGTGGCAAAACCCTGGTGATGGCTGTGTATGACTTTGATCGCT
TCTCCAAGCATGACATCATCGGAGAGTTCAAAGTTCCTATGAACACCGTGGATTTTGGCCATGTGACCGA
GGAGTGGCGTGATCTCCAGAGCGCTGAGAAAGAAGAGCAAGAGAAACTGGGTGACATCTGCTTCTCCCTC
CGCTACGTCCCTACTGCCGGCAAACTGACTGTTGTCATTCTGGAAGCCAAGAACCTGAAGAAGATGGATG
TGGGTGGCTTATCTGATCCCTACGTGAAGATTCACCTGATGCAGAACGGTAAGAGGCTGAAGAAGAAAAA
GACGACGATTAAGAAGAACACACTCAACCCCTACTACAACGAGTCCTTCAGCTTTGAAGTTCCGTTCGAG
CAAATCCAGAAAGTGCAAGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGACGCCATCG
GCAAAGTCTTCGTTGGTTACAACAGCACTGGGGCGGAGCTGCGACACTGGTCAGACATGCTGGCCAACCC
CCGGCGACCCATCGCACAGTGGCACACTCTGCAGGTAGAGGAGGAGGTTGATGCCATGCTGGCTGTCAAG
AAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR203399 representing NM_001033680
Red=Cloning site Green=Tags(s)

MVSASHPEALAAPVTTVATLVPHNATEPASPGEGKEDAFSKLKQKFMNELHKIPLPPWALIAIAIVAVLL
VVTCCFCVCKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEE
KLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQF
TFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSL
RYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFE
QIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVK
KK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001033680
ORF Size 1263 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001033680.1, NM_001033680.2, NP_001028852.2
RefSeq Size 1896 bp
RefSeq ORF 1266 bp
Locus ID 25716
Cytogenetics 7q21
MW 47.4 kDa
Gene Summary integral component of synaptic vesicle membranes; important for exocytosis and vesicle trafficking to the active zone of synapses; may be the calcium sensor for calcium-dependent exocytosis [RGD, Feb 2006]

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