Syt1 (NM_001033680) Rat Tagged ORF Clone

CAT#: RR203399L4

Lenti ORF clone of Syt1 (mGFP-tagged ORF) - Rat synaptotagmin I (Syt1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: mGFP
• Symbol: Syt1
• Synonyms: P65
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RR203399).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_001033680
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033680.2, NP_001028852.2
• RefSeq Size: 1896 bp
• RefSeq ORF: 1266 bp
• Locus ID: 25716
• Cytogenetics: 7q21
• Gene Summary: integral component of synaptic vesicle membranes; important for exocytosis and vesicle trafficking to the active zone of synapses; may be the calcium sensor for calcium-dependent exocytosis [RGD, Feb 2006]