Ogt (NM_017107) Rat Tagged ORF Clone

CAT#: RR203412

Ogt (Myc-DDK-tagged ORF) - Rat O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (Ogt), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ogt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203412 representing NM_017107
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTTCCGTGGGCAACGTGGCCGACAGCACAGGGTTAGCTGAGTTGGCACATCGGGAATATCAGG
  CAGGAGACTTTGAGGCAGCTGAGAGACACTGCATGCAGCTCTGGAGACAAGAGCCTGACAATACTGGTGT
  TCTTTTATTACTTTCATCTATACATTTCCAGTGTCGAAGGCTGGACAGATCTGCTCACTTTAGCACCTTG
  GCAATTAAACAGAATCCCCTTCTAGCAGAAGCCTATTCGAATTTGGGAAATGTATACAAGGAAAGAGGGC
  AATTACAGGAAGCAATTGAGCATTATCGACATGCTTTGCGGCTCAAGCCTGATTTCATTGATGGTTATAT
  TAACCTGGCAGCAGCCTTGGTAGCAGCAGGTGACATGGAGGGAGCAGTGCAAGCATACGTCTCTGCTCTT
  CAGTACAATCCTGATTTGTACTGTGTTCGCAGTGACCTGGGGAACCTGCTCAAAGCCCTGGGTCGCTTGG
  AAGAAGCCAAGGCATGTTATTTGAAAGCAATTGAGACGCAACCAAACTTTGCTGTAGCCTGGAGTAATCT
  CGGCTGTGTTTTCAATGCACAAGGGGAGATTTGGCTGGCAATTCATCACTTTGAAAAGGCTGTCACCCTT
  GACCCAAATTTTCTGGATGCTTATATCAATTTAGGAAATGTTTTGAAAGAGGCACGGATTTTTGACAGAG
  CTGTGGCAGCTTATCTTCGTGCCTTAAGTTTGAGCCCAAATCATGCGGTGGTACACGGCAACCTGGCTTG
  TGTGTACTATGAGCAAGGCCTTATAGACCTGGCCATTGATACCTACAGGCGAGCTATAGAACTGCAGCCT
  CATTTCCCTGATGCATACTGCAACCTAGCAAATGCTCTCAAAGAGAAGGGCAGTGTTGCTGAAGCAGAAG
  ATTGTTATAACACAGCTCTTCGTCTGTGTCCTACCCATGCAGACTCTTTGAATAACCTTGCCAACATCAA
  ACGGGAACAGGGCAATATTGAAGAGGCAGTTCGCCTGTATCGCAAAGCATTAGAGGTCTTCCCAGAGTTT
  GCTGCTGCACATTCCAATTTAGCAAGTGTACTGCAACAGCAGGGCAAGCTGCAGGAAGCACTGATGCACT
  ATAAGGAAGCCATAAGAATCAGTCCTACATTTGCTGATGCTTATTCCAATATGGGAAACACTTTAAAGGA
  GATGCAGGATGTTCAGGGAGCTTTGCAGTGTTATACTCGTGCCATCCAGATTAATCCTGCCTTCGCAGAT
  GCACACAGCAATCTGGCTTCCATTCACAAGGATTCAGGGAATATTCCAGAAGCAATAGCTTCTTACCGCA
  CAGCTCTGAAACTTAAGCCTGACTTTCCTGATGCTTATTGTAACTTGGCTCATTGCCTACAGATTGTCTG
  TGATTGGACAGACTATGATGAGCGGATGAAGAAATTGGTTAGTATTGTAGCTGAGCAGCTAGAGAAGAAT
  AGGCTGCCTTCTGTCCATCCTCATCACAGCATGCTGTACCCTCTTTCCCATGGCTTCAGGAAGGCCATTG
  CAGAGAGGCATGGGAATCTCTGCTTGGATAAGATTAATGTCCTTCATAAACCACCATATGAGCATCCAAA
  AGACTTGAAGCTCAGTGATGGCCGATTGCGTGTAGGCTATGTGAGTTCTGACTTTGGGAATCATCCTACT
  TCACACCTTATGCAGTCTATTCCAGGCATGCACAACCCTGATAAGTTTGAGGTATTCTGCTATGCCTTGA
  GCCCGGATGACGGTACAAACTTCCGAGTGAAGGTGATGGCGGAAGCCAATCACTTCATTGATCTTTCTCA
  GATTCCGTGCAATGGAAAAGCAGCTGACCGCATCCACCAAGATGGAATTCACATCCTTGTCAATATGAAT
  GGGTACACCAAGGGTGCCCGAAATGAGCTCTTTGCTCTCAGGCCAGCTCCTATTCAGGCTATGTGGCTGG
  GCTACCCTGGGACTAGTGGCGCACTGTTCATGGATTACATCATCACTGATCAGGAAACTTCCCCAGCTGA
  AGTTGCAGAGCAGTATTCTGAAAAACTGGCTTATATGCCCCATACGTTCTTTATTGGTGATCATGCTAAT
  ATGTTCCCACACCTGAAGAAAAAAGCAGTCATCGATTTTAAATCTAATGGGCACATTTATGACAATCGAA
  TAGTTTTGAATGGCATCGATCTCAAAGCATTTCTCGATAGTCTACCCGATGTGAAGATTGTCAAGATGAA
  ATGTCCTGATGGAGGTGACAATGCAGACACCACTAACACAGCTCTTAACATGCCCGTTATTCCCATGAAT
  ACGATTGCAGAAGCAGTAATTGAAATGATTAACAGAGGGCAGATTCAGATAACAATTAATGGATTCAGTA
  TTAGCAATGGACTGGCTACTACACAGATCAATAACAAGGCTGCAACTGGAGAGGAAGTTCCCCGTACCAT
  TATTGTAACCACCCGTTCCCAGTACGGGCTACCAGAAGATGCCATTGTGTACTGTAACTTTAATCAGTTA
  TATAAAATTGACCCTTCTACCCTGCAGATGTGGGCAAATATTCTGAAACGTGTGCCTAATAGCGTGCTTT
  GGCTGTTGCGTTTTCCAGCAGTAGGAGAGCCCAATATTCAACAATATGCACAAAATATGGGCCTTCCCCA
  GAACCGTATCATTTTCTCACCTGTGGCTCCTAAAGAGGAGCATGTCAGGAGAGGTCAGCTGGCTGATGTC
  TGCCTGGATACTCCTTTGTGTAATGGACATACCACAGGGATGGATGTTCTCTGGGCAGGAACACCCATGG
  TGACTATGCCAGGAGAGACTCTTGCCTCTCGAGTTGCAGCTTCTCAGCTCACTTGTCTAGGATGTCTTGA
  GCTCATTGCTAAAAGCAGACAGGAATATGAAGACATAGCCGTGAAACTGGGGACCGATCTAGAATACCTG
  AAGAAAATTCGTGGCAAAGTATGGAAACAGAGAATATCTAGCCCTCTGTTCAACACCAAACAATACACAA
  TGGAATTAGAGCGGCTGTATCTGCAGATGTGGGAGCATTATGCAGCTGGCAACAAACCCGACCACATGAT
  TAAGCCTGTTGAAGTCACTGAGTCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203412 representing NM_017107
  Red=Cloning site Green=Tags(s)
  
  MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTL
  AIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSAL
  QYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTL
  DPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQP
  HFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEF
  AAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFAD
  AHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVAEQLEKN
  RLPSVHPHHSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPT
  SHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIHILVNMN
  GYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMPHTFFIGDHAN
  MFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNADTTNTALNMPVIPMN
  TIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQL
  YKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADV
  CLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKSRQEYEDIAVKLGTDLEYL
  KKIRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPVEVTESA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017107
• ORF Size: 3108 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017107.1, NM_017107.2, NP_058803.2
• RefSeq Size: 5308
• RefSeq ORF: 3111
• Locus ID: 26295
• MW: 115.7 kDa
• Gene Summary: the catalytic p110 subunit of the enzyme involved in the dynamic O-GlcNAcylation of nuclear and cytosolic proteins [RGD, Feb 2006]