C1rl (NM_001002804) Rat Tagged ORF Clone

CAT#: RR203459

C1rl (Myc-DDK-tagged ORF) - Rat complement component 1, r subcomponent-like (C1rl), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1rl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203459 representing NM_001002804
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTGGTTGCTCCTTTGGGGAATCCTCCATACTTGCCCCACACAGGCCTCTGTGCTTTTAGCCCAGC
  AGTTTCCTCAGCAACTGACATCCCCCGGATACCCAGAGCCATATATCAAAGGCCAGGAGAGCCATGCTGA
  CATCGAGGCCCCGGAGGGGTTTGCCGTGAGACTGATCTTCCAGGACTTTGATCTGGAGCCGTCCCCAGGC
  TGTGAAGGCGACTCTGTCACCATTTCAACCAGAGGAACAGATGCAACCCGGCTGTGTGGGCAGCAAGGTT
  CCTCTCTGGGGAGCCCTCCCAATCAAATGGAGTTTGTATCCTCCGGGAGGAGCCTTCGGCTGACCTTCCG
  GGCACACTCCTCCAAGAACAAAGTCACACACCTCCACAAAGGCTTCCTGGCTCTCTACCAAGCCGCTGTG
  AGCCAACCCAATGGGGACGCTGAGGCTTTCACCACACCTGGAGCCAACCCTCCCGAGATTCAGAACCACT
  GCCCAGGTCCCTATTATAAGGAAGAACAGACAGGGACACTTAGCTGCCCATCCTCTCGGAAGTGGAAGGA
  CAGACAGCGTGGAGAAGAGGTTCCTGAGTGTGTGCCGGTCTGTGGAAGACCAGTTGTCCCCATTGCCGAG
  AACCCAAACACTTTTGGTTCTTCCAGAGCCAAGCCTGGCAACTTCCCCTGGCAAGCCTTCACCAGTATCT
  ATGGCCGTGGGGGCGGAGCCCTGCTGGGTGACAGATGGATCCTGACGGCTGCCCATACCATCTTCCCCAA
  GGATAGCATCTATCTCAGAAAGAACAAGACTGTGAATGTGTTTCTGGGTCACACAGACGTAGACGAGCTG
  CTGAAACTGGGGAACCATCCTGTCCGAAGGGTGGTGGTTCACCCAGACTACCGCCAAGAGGAGTCCCACA
  ACTTTGATGGGGATATCGCCCTCCTGGAGCTTGAGCACAGGGTCCCTCTGGGGCCCAGTCTCCTCCCGGT
  CTGTCTGCCGGACAACGAGACCCTCTACCACAGCGGCCTGTGGGGTTACATCAGTGGGTTCGGTGTGGAG
  ATGGGCTGGTTGACGACAAAGTTGAAATACTCAAAGCTGCCTGTAGCTCCCCGGGAGGCCTGTGAAGCCT
  GGCTCCGCCAGAGGCAGCGAACTGAGGTGTTTTCTGACAATATGTTCTGTGTCGGGGAAGAGATGCAGGT
  CAACAGTGTCTGCCAGGGAGACAGTGGCAGCGTCTACGTGGTGTGGGACGACCGTGCTCTTCGCTGGGTG
  GCCACGGGCATTGTATCCTGGGGCGTCGGGTGTGGCAAGGGGTACGGCTTCTACACCAAAGTGCTCAGCT
  ACGTGGATTGGATTAAGGGGGTGATTGAATGTAAAGACCGATGCCCGGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203459 representing NM_001002804
  Red=Cloning site Green=Tags(s)
  
  MCWLLLWGILHTCPTQASVLLAQQFPQQLTSPGYPEPYIKGQESHADIEAPEGFAVRLIFQDFDLEPSPG
  CEGDSVTISTRGTDATRLCGQQGSSLGSPPNQMEFVSSGRSLRLTFRAHSSKNKVTHLHKGFLALYQAAV
  SQPNGDAEAFTTPGANPPEIQNHCPGPYYKEEQTGTLSCPSSRKWKDRQRGEEVPECVPVCGRPVVPIAE
  NPNTFGSSRAKPGNFPWQAFTSIYGRGGGALLGDRWILTAAHTIFPKDSIYLRKNKTVNVFLGHTDVDEL
  LKLGNHPVRRVVVHPDYRQEESHNFDGDIALLELEHRVPLGPSLLPVCLPDNETLYHSGLWGYISGFGVE
  MGWLTTKLKYSKLPVAPREACEAWLRQRQRTEVFSDNMFCVGEEMQVNSVCQGDSGSVYVVWDDRALRWV
  ATGIVSWGVGCGKGYGFYTKVLSYVDWIKGVIECKDRCPEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002804
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002804.1, NP_001002804.1
• RefSeq Size: 1386 bp
• RefSeq ORF: 1386 bp
• Locus ID: 408246
• Cytogenetics: 4q42
• MW: 51.1 kDa
• Gene Summary: human homolog catalyzes the cleavage of the haptoglobin precursor protein [RGD, Feb 2006]