Melk (NM_001108662) Rat Tagged ORF Clone

CAT#: RR203510

Melk (Myc-DDK-tagged ORF) - Rat maternal embryonic leucine zipper kinase (Melk), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Melk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203510 representing NM_001108662
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGATTATGACGAGCTCCTTAAATACTATGAATTATATGAAACTATTGGGACAGGTGGCTTTGCAA
  AGGTCAAACTTGCCTGTCATATCCTCACTGGAGAAATGGTAGCTATAAAAATCATGGATAAGAATGCACT
  AGGGAGCGATTTGCCCCGAGTCAAAACTGAAATTGATGCCCTGAAGAATCTGAGACATCAGCATATATGT
  CAGCTCTACCATGTGCTAGAGACAAAGAACAAAATATTCATGGTGCTTGAGTACTGTCCAGGAGGAGAGC
  TGTTTGACTACATCATCTCCCAGGATCGCCTGTCGGAAGAGGAGACCCGAGTCATCTTCCGCCAGATCCT
  GTCTGCAGTTGCATATGTGCACAGCCAGGGCTATGCCCACAGGGACCTCAAGCCAGAAAATTTATTGTTT
  GACGAAAATCATAAGCTAAAGCTGATTGACTTTGGTCTCTGTGCAAAACCCAAGGGCAACAAGGACTACC
  ATCTACAGACATGCTGTGGGAGTCTGGCGTATGCAGCACCTGAACTAATACAAGGGAAATCGTATCTTGG
  ATCAGAGGCAGATGTTTGGAGCATGGGCATCCTCCTATATGTGCTTATGTGTGGATTCCTCCCGTTTGAT
  GATGATAATGTCATGGCTTTGTACAAGAAGATAATGAGAGGGAAGTATGAAGTTCCCAAGTGGCTGTCTC
  CCAGCAGCATCCTGCTTCTCCAGCAGATGCTTCAGGTGGACCCAAAGAGACGGATTTCTATGAAAAATCT
  CTTGAACCATCCCTGGATCCTGCAAGATTACAGCTGTCCCATTGAGTGGCAAAGCAAGGCTTCTTTGACT
  CACCTTGATGAGGATTGCATAACAGAGCTTTCTGTACATCACCGCAACAGCAGGCAAACAATGGAGGATT
  TAATTTCGTCGTGGCAGTATGATCACCTCACAGCCACCTACCTTCTGCTTCTAGCCAAGAAGGCTCGAGG
  GAAGCCAGCTCGTCTGCAGCTTCTGTCCTTCTCTGGTGGGACCACCAGCACCACTCCAAAGGCAATCAAA
  GAACCTGAGCCTGGAAGATATGTCCACAAGTGGCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203510 representing NM_001108662
  Red=Cloning site Green=Tags(s)
  
  MKDYDELLKYYELYETIGTGGFAKVKLACHILTGEMVAIKIMDKNALGSDLPRVKTEIDALKNLRHQHIC
  QLYHVLETKNKIFMVLEYCPGGELFDYIISQDRLSEEETRVIFRQILSAVAYVHSQGYAHRDLKPENLLF
  DENHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFD
  DDNVMALYKKIMRGKYEVPKWLSPSSILLLQQMLQVDPKRRISMKNLLNHPWILQDYSCPIEWQSKASLT
  HLDEDCITELSVHHRNSRQTMEDLISSWQYDHLTATYLLLLAKKARGKPARLQLLSFSGGTTSTTPKAIK
  EPEPGRYVHKWR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108662
• ORF Size: 1086 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001108662.1, NP_001102132.1
• RefSeq Size: 2842
• RefSeq ORF: 1089
• Locus ID: 362510
• MW: 41.6 kDa