Tuba4a (NM_001007004) Rat Tagged ORF Clone

CAT#: RR203662

Tuba4a (Myc-DDK-tagged ORF) - Rat tubulin, alpha 4A (Tuba4a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tuba4a
• Synonyms: Tuba4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203662 representing NM_001007004
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTGAGTGCATTTCAGTCCATGTGGGCCAGGCAGGTGTCCAGATGGGCAATGCCTGCTGGGAGCTCT
  ACTGCCTGGAACATGGGATTCAGCCTGATGGGCAGATGCCTAGCGATAAGACCATTGGTGGAGGGGATGA
  CTCCTTCACCACCTTCTTCTGTGAAACTGGAGCTGGAAAACATGTGCCTCGGGCAGTCTTTGTGGATCTG
  GAGCCTACTGTAATTGATGAGATCCGAAATGGCCCGTACCGCCAGCTCTTCCACCCAGAACAGCTTATCA
  CTGGGAAGGAAGATGCAGCCAACAACTATGCCCGTGGTCACTACACCATTGGCAAGGAGATCATCGACCC
  AGTGCTGGACCGGATCCGCAAGCTGTCTGATCAGTGTACAGGACTCCAGGGCTTTCTGGTGTTCCACAGC
  TTTGGAGGAGGCACTGGCTCTGGCTTCACCTCTCTGCTGATGGAACGGCTTTCTGTTGACTATGGCAAGA
  AGTCCAAGCTGGAGTTCTCCATCTACCCAGCCCCACAAGTGTCCACGGCTGTAGTGGAGCCCTATAACTC
  CATCCTGACCACCCATACCACCCTGGAACACTCGGACTGTGCCTTCATGGTGGACAACGAGGCCATTTAT
  GACATCTGTCGCCGTAACCTGGATATTGAGCGCCCAACCTATACCAACCTCAACCGCCTCATCAGCCAAA
  TTGTCTCCTCCATCACAGCTTCCTTGCGCTTTGATGGGGCCCTCAATGTGGACCTGACAGAGTTCCAGAC
  CAACCTGGTACCCTACCCTCGCATCCACTTCCCCTTGGCTACCTACGCACCAGTCATTTCTGCAGAGAAG
  GCCTACCATGAGCAGCTGTCAGTGGCAGAGATCACCAATGCCTGTTTTGAGCCTGCCAACCAGATGGTGA
  AATGTGACCCTCGGCATGGCAAGTACATGGCCTGCTGCCTGCTGTACCGGGGAGATGTGGTGCCCAAGGA
  TGTGAACGCTGCCATCGCTGCCATCAAGACGAAGCGCAGCATCCAGTTTGTGGACTGGTGCCCCACAGGC
  TTCAAGGTCGGTATCAACTACCAGCCTCCCACCGTGGTGCCTGGGGGCGACCTGGCCAAGGTGCAGCGTG
  CAGTGTGCATGCTGAGCAACACAACCGCCATTGCTGAAGCCTGGGCCCGCCTGGACCACAAGTTTGACTT
  GATGTATGCCAAGAGGGCTTTTGTACACTGGTATGTGGGTGAGGGAATGGAGGAGGGGGAGTTCTCCGAG
  GCTCGAGAGGATATGGCTGCCCTGGAGAAAGATTATGAGGAAGTAGGCATCGACTCCTATGAGGACGAGG
  ATGAGGGAGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203662 representing NM_001007004
  Red=Cloning site Green=Tags(s)
  
  MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDL
  EPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHS
  FGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY
  DICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK
  AYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTG
  FKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
  AREDMAALEKDYEEVGIDSYEDEDEGEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007004
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007004.1, NP_001007005.1
• RefSeq Size: 1477 bp
• RefSeq ORF: 1347 bp
• Locus ID: 316531
• Cytogenetics: 9q33
• MW: 49.9 kDa