Vcl (NM_001107248) Rat Tagged ORF Clone

CAT#: RR203691

Vcl (Myc-DDK-tagged ORF) - Rat vinculin (Vcl), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vcl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203691 representing NM_001107248
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTGTTTCACACGCGCACGATCGAGAGCATCCTGGAGCCGGTGGCGCAGCAGATCTCTCACCTGG
  TGATTATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTCACCGCGCCCGTGGCCGCTGT
  GCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGGAAAGAGACCGTTCAGACCACTGAGGATCAGATTTTG
  AAGAGAGATATGCCGCCAGCATTTATTAAGGTCGAGAATGCTTGCACGAAGCTTGTTCAGGCAGCTCAGA
  TGCTTCAGTCAGACCCATACTCTGTTCCTGCGCGGGATTACCTCATCGACGGCTCCAGGGGAATCCTCTC
  TGGAACGTCTGACCTCCTGCTTACCTTTGATGAGGCTGAGGTTCGAAAAATTATAAGAGTTTGCAAAGGG
  ATTTTGGAATACCTCACAGTGGCAGAGGTAGTGGAGACTATGGAAGACTTGGTCACTTACACAAAGAACC
  TTGGGCCCGGAATGACGAAGATGGCCAAAATGATCGATGAGAGACAGCAGGAGTTGACGCACCAGGAACA
  CCGTGTGATGCTGGTGAACTCAATGAACACTGTCAAAGAGCTGCTTCCTGTTCTCATTTCAGCTATGAAG
  ATTTTTGTTACAACCAAAAACTCAAAAAACCAAGGAATAGAAGAAGCTCTGAAAAATCGAAATTTTACTG
  TAGGAAAGATGAGTGCTGAGATTAACGAGATCATTCGTGTGTTACAACTCACCTCCTGGGATGAGGACGC
  CTGGGCCAGCAAGGACACTGAAGCCATGAAGAGAGCGCTGGCCTCCATAGACTCGAAACTGAACCAGGCC
  AAAGGTTGGCTCCGTGACCCCAACGCTTCCCCAGGGGACGCCGGTGAGCAGGCCATCAGGCAGATCTTGG
  ACGAAGCTGGAAAAGTTGGTGAACTTTGTGCAGGCAAGGAACGCAGGGAGATCCTAGGAACCTGCAAGAT
  GCTAGGGCAGATGACTGACCAAGTGGCTGACCTCCGAGCCAGAGGACAAGGAGCCTCGCCAGTGGCCATG
  CAGAAGGCCCAGCAAGTGTCTCAGGGGCTCGACGTGCTTACTGCGAAAGTGGAAAATGCAGCTCGAAAGC
  TGGAAGCCATGACGAACTCAAAGCAGAGTATTGCGAAGAAGATTGACGCTGCGCAGAATTGGCTGGCAGA
  TCCCAATGGTGGACCTGAAGGAGAAGAACAGATCCGAGGGGCTTTGGCTGAAGCTCGGAAGATTGCTGAG
  TTATGTGATGATCCTAAGGAGAGGGACGACATCCTCCGCTCTCTTGGAGAGATAGCGGCTCTGACCTCTA
  AATTAGGAGACTTGCGAAGACAGGGGAAAGGAGACTCCCCAGAGGCTCGAGCCTTGGCTAAACAAGTGGC
  AACCGCGCTACAGAACCTGCAGACCAAAACCAATAGGGCTGTGGCCAACAGCAGGCCTGCCAAGGCAGCC
  GTCCACCTGGAAGGCAAGATTGAGCAGGCACAGCGGTGGATTGATAACCCTACAGTGGATGACCGAGGAG
  TCGGTCAGGCTGCCATCCGTGGACTTGTGGCTGAAGGGCATCGGCTGGCCAATGTCATGATGGGACCTTA
  TCGCCAAGATCTTCTCGCCAAGTGTGACCGTGTAGACCAGCTAGCAGCTCAGCTGGCTGACCTGGCTGCC
  CGAGGGGAGGGGGAGAGTCCTCAGGCGAGAGCGCTTGCTTCTCAGCTTCAGGACTCCTTAAAGGATCTTA
  AAACCCAGATGCAGGAAGCTATGACTCAAGAGGTGTCCGACGTTTTCAGCGATACTACAACTCCCATCAA
  GCTGTTGGCAGTGGCGGCCACTGCTCCTCCTGATGCGCCCAATAGGGAAGAGGTATTTGACGAGAGAGCA
  GCCAACTTTGAAAACCATTCAGGAAGGCTTGGGGCCACGGCAGAGAAGGCAGCTGCTGTTGGTGCTGCCA
  ATAAATCGACAGTGGAAGGCATTCAGGCATCCGTGAAGACAGCCCGAGAACTCACTCCCCAGGTGATCTC
  AGCTGCTCGAATCTTACTGAGGAACCCTGGTAACCAAGCTGCGTATGAACATTTTGAGACCATGAAGAAC
  CAGTGGATTGATAATGTTGAGAAAATGACAGGGCTGGTGGACGAGGCCATTGATACCAAATCTCTGTTGG
  ATGCTTCTGAAGAAGCAATTAAAAAAGACCTGGACAAGTGTAAGGTAGCCATGGCCAATATTCAGCCTCA
  GATGCTGGTCGCTGGAGCAACCAGTATTGCTCGTCGGGCCAACCGGATTCTGCTGGTTGCTAAGAGGGAG
  GTAGAGAACTCTGAGGATCCGAAGTTCCGAGAGGCTGTGAAAGCTGCCTCTGATGAACTGAGCAAAACAA
  TCTCCCCCATGGTTATGGATGCCAAGGCTGTGGCTGGAAACATCTCTGACCCTGGCCTGCAAAAGAGCTT
  CCTGGACTCAGGATATCGGATCCTGGGAGCTGTGGCCAAAGTTAGAGAAGCCTTCCAACCTCAGGAGCCT
  GACTTCCCGCCTCCTCCACCAGACCTTGAACAGCTACGTCTAACTGATGAGCTGGCTCCTCCCAAACCAC
  CTCTGCCTGAGGGTGAAGTCCCTCCACCCAGGCCACCCCCACCAGAAGAGAAGGATGAAGAGTTCCCTGA
  GCAGAAAGCCGGGGAGGTGATTAACCAGCCGATGATGATGGCCGCCAGGCAGCTCCACGATGAAGCTCGG
  AAGTGGTCTAGCAAGGGCAATGACATCATCGCAGCAGCCAAGCGCATGGCTCTGCTGATGGCGGAGATGT
  CTCGGCTGGTAAGAGGGGGCAGTGGTACCAAGCGGGCACTTATTCAGTGTGCCAAGGATATCGCCAAGGC
  CTCTGATGAGGTGACGAGGTTGGCCAAGGAGGTTGCCAAGCAGTGCACAGATAAGCGGATTAGAACCAAT
  CTCTTACAGGTCTGTGAGCGAATCCCAACTATAAGCACCCAGCTCAAAATCCTGTCCACAGTGAAGGCCA
  CCATGCTGGGCCGGACCAACATCAGTGACGAGGAGTCTGAGCAGGCCACAGAGATGCTGGTTCATAATGC
  CCAGAACCTCATGCAGTCTGTGAAAGAGACTGTGCGAGAGGCCGAAGCTGCTTCAATCAAGATTCGAACA
  GACGCCGGATTTACTCTGCGCTGGGTCAGAAAGACTCCCTGGTACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203691 representing NM_001107248
  Red=Cloning site Green=Tags(s)
  
  MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQIL
  KRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKG
  ILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMK
  IFVTTKNSKNQGIEEALKNRNFTVGKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQA
  KGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAM
  QKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAE
  LCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAA
  VHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLAAQLADLAA
  RGEGESPQARALASQLQDSLKDLKTQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERA
  ANFENHSGRLGATAEKAAAVGAANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKN
  QWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKRE
  VENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEP
  DFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEAR
  KWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTN
  LLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRT
  DAGFTLRWVRKTPWYQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107248
• ORF Size: 3198 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001107248.1, NP_001100718.1
• RefSeq Size: 5270
• RefSeq ORF: 3201
• Locus ID: 305679
• MW: 116.6 kDa