Echs1 (NM_078623) Rat Tagged ORF Clone

CAT#: RR203801

Echs1 (Myc-DDK-tagged ORF) - Rat enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Echs1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203801 representing NM_078623
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCTGCGTGCTCTGCTGCCCAGAGCCTGCAACTCGCTGTTGTCCCCAGTTCGCTGCCCAGAAT
  TCCGGCGCTTCGCCTCGGGTGCTAACTTTCAGTACATCATCACGGAAAAGAAAGGAAAGAATAGCAGCGT
  GGGGCTGATCCAGTTGAACCGTCCCAAAGCACTCAATGCACTTTGCAATGGACTGATTGAGGAGCTCAAC
  CAAGCACTGGAGACCTTTGAGGAAGATCCCGCTGTGGGCGCCATTGTGCTCACTGGTGGGGAGAAGGCCT
  TTGCAGCCGGAGCTGACATCAAGGAAATGCAGAACCGGACATTTCAGGACTGTTACTCAGGCAAGTTCCT
  GAGCCACTGGGACCATATCACCCGGATCAAGAAACCGGTCATCGCGGCTGTCAATGGCTATGCTCTTGGT
  GGGGGCTGTGAACTTGCCATGATGTGCGATATCATCTATGCTGGTGAGAAAGCCCAGTTTGGACAGCCAG
  AAATCCTCCTGGGGACCATCCCAGGTGCAGGGGGCACTCAGAGACTCACCCGAGCAGTCGGCAAATCACT
  AGCAATGGAGATGGTCCTCACTGGTGACCGAATTTCAGCACAGGATGCCAAGCAAGCAGGTCTTGTAAGC
  AAGATTTTTCCCGTTGAAACACTGGTTGAAGAGGCCATCCAATGTGCAGAAAAGATCGCCAACAATTCCA
  AGATCATAGTAGCCATGGCGAAAGAATCTGTGAATGCAGCCTTTGAAATGACGTTAACAGAAGGAAATAA
  GCTGGAGAAGAAGCTCTTCTATTCCACCTTTGCCACTGATGACCGGAGAGAAGGGATGTCTGCCTTTGTG
  GAGAAAAGGAAGGCCAACTTCAAAGACCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203801 representing NM_078623
  Red=Cloning site Green=Tags(s)
  
  MAALRALLPRACNSLLSPVRCPEFRRFASGANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELN
  QALETFEEDPAVGAIVLTGGEKAFAAGADIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALG
  GGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS
  KIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFV
  EKRKANFKDH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_078623
• ORF Size: 870 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_078623.1, NM_078623.2, NP_511178.1
• RefSeq Size: 1560
• RefSeq ORF: 873
• Locus ID: 140547
• MW: 31.5 kDa
• Gene Summary: catalyzes the conversion of (3S)-3-hydroxyacyl-CoA to trans-2(or 3)-enoyl-CoA and H2O in fatty acid beta-oxidation [RGD, Feb 2006]