Aldh1b1 (NM_001011975) Rat Tagged ORF Clone

CAT#: RR203802

Aldh1b1 (Myc-DDK-tagged ORF) - Rat aldehyde dehydrogenase 1 family, member B1 (Aldh1b1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh1b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203802 representing NM_001011975
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAACGCCCGATTCTTGGTGCCCCGGCTCCTCTGCCTCCAGGGCAGGACCACCTCATATTCTACAG
  CGGCTGCTCTCCCAAACCCAATCCCAAACCCAGAGATTCGCTACAACCAGCTGTTCATCAACAACGAGTG
  GCATGATGCGGTCAGCAAAAAGACCTTCCCCACGGTGAACCCCACGACAGGCGAGGTCATTGGGCATGTG
  GCTGAAGGTGACCGGGCAGATGTGGATCTGGCTGTGAGAGCAGCCCGAGAAGCCTTCCGCCTGGGGTCCC
  CGTGGCGCAGGATGGATGCCTCAGAGCGGGGCCGGCTGCTGAACCGCCTAGCTGATCTCGTGGAACGGGA
  TCGAGTGTACTTGGCCTCACTGGAGACCCTGGATAATGGGAAACCTTTTCAGGAGTCTTATGTCTTGGAC
  CTGGATGAAGTCATCAAGGTATACCGTTACCTTGCTGGCTGGGCTGACAAATGGCATGGTAAGACCATCC
  CAATGGACGGCGAACATTTCTGCTTCACCCGCCATGAGCCAGTGGGTGTCTGTGGCCAGATAATCCCTTG
  GAACTTCCCATTGGTCATGCAGGGTTGGAAGCTGGCCCCAGCACTCGCCACAGGCAACACCGTGGTCATG
  AAGGTGGCAGAGCAAACCCCACTTTCTGCTCTGTACTTGGCCTCCCTCATCAAGGAAGCGGGGTTTCCCC
  CAGGAGTGGTGAATATCATCACCGGCTACGGCCCCACTGCAGGAGCAGCCATTGCCCAGCACATGGATGT
  GGATAAAGTCGCCTTCACCGGCTCTACCGAGGTGGGCCACCTGATTCAGAAGGCCGCCGGTGATTCCAAC
  CTCAAGAGAGTCACCCTGGAGCTGGGTGGGAAAAGCCCCAGCATCGTGCTGGCAGACGCTGACATGGATC
  ACGCCGTAGACCAGTGTCACGAAGCCCTCTTTTTCAACATGGGTCAGTGCTGCTGTGCAGGATCCCGGAC
  ATTCGTGGAAGAGTCCATCTATCATGAGTTTCTCGAGAGAACTGTGGAGAAGGCCAAGAAGAGGAAAGTG
  GGGAACCCCTTTGAGTTGGACACTCAACAGGGACCTCAGGTGGACAAGGAGCAATTTGAAAAGATCCTGG
  GCTACATCCGGCTTGGACAGAAGGAAGGGGCAAAGCTTCTCTGTGGTGGGGAGCGTTTCGGGGAACGCGG
  CTTCTTCATCAAGCCCACAGTCTTTGGGAATGTTCAGGATGACATGAGGATCGCCAGGGAGGAGATCTTT
  GGGCCTGTGCAGCCTCTGTTCAAGTTCAAGAAGATCGAGGAAGTAATCCAGAGAGCCAACAATACCAGGT
  ACGGCTTGGCTGCGGCTGTATTCACCCGAGACCTGGACAAGGCCTTATACTTTTCCCAGGCCCTGCAAGC
  CGGGACCGTGTGGGTGAACACCTACAATATCGTCACCTGCCACACGCCATTCGGAGGCTTTAAGGAATCC
  GGCAATGGCAGAGAGCTGGGGGAAGATGGGCTTAAAGCCTACACGGAGGTGAAGACTGTCACCATCAAGG
  TTTCTGAGAAGAATTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203802 representing NM_001011975
  Red=Cloning site Green=Tags(s)
  
  MLNARFLVPRLLCLQGRTTSYSTAAALPNPIPNPEIRYNQLFINNEWHDAVSKKTFPTVNPTTGEVIGHV
  AEGDRADVDLAVRAAREAFRLGSPWRRMDASERGRLLNRLADLVERDRVYLASLETLDNGKPFQESYVLD
  LDEVIKVYRYLAGWADKWHGKTIPMDGEHFCFTRHEPVGVCGQIIPWNFPLVMQGWKLAPALATGNTVVM
  KVAEQTPLSALYLASLIKEAGFPPGVVNIITGYGPTAGAAIAQHMDVDKVAFTGSTEVGHLIQKAAGDSN
  LKRVTLELGGKSPSIVLADADMDHAVDQCHEALFFNMGQCCCAGSRTFVEESIYHEFLERTVEKAKKRKV
  GNPFELDTQQGPQVDKEQFEKILGYIRLGQKEGAKLLCGGERFGERGFFIKPTVFGNVQDDMRIAREEIF
  GPVQPLFKFKKIEEVIQRANNTRYGLAAAVFTRDLDKALYFSQALQAGTVWVNTYNIVTCHTPFGGFKES
  GNGRELGEDGLKAYTEVKTVTIKVSEKNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011975
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011975.1, NP_001011975.1
• RefSeq Size: 2221 bp
• RefSeq ORF: 1560 bp
• Locus ID: 298079
• Cytogenetics: 5q22
• MW: 57.6 kDa