Me1 (NM_012600) Rat Tagged ORF Clone

CAT#: RR203845

Me1 (Myc-DDK-tagged ORF) - Rat malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Me1
• Synonyms: MOD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203845 representing NM_012600
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCCCGAGCCCCCCGACGCCGACACGCCCACCAGCGCGGCTACCTGCTGACGCGGGACCCGCATC
  TCAACAAGGACTTGGCTTTTACTCTGGAAGAGAGGCAACAGCTGAACATTCATGGCTTGTTGCCACCCTG
  CATTGTCAACCAGGAGATCCAGGTCCTTAGAGTAATTAAGAATTTCGAGCGTCTGAACTCTGACTTCGAC
  AGGTATCTTCTGTTAATGGATCTGCAAGATAGGAATGAGAAGCTCTTCTACAGTGTGCTTATGTCTAATG
  TTGAAAAGTTCATGCCTATCGTTTACACTCCCACCGTGGGTCTTGCATGCCAACAATACAGTTTGGCATT
  CCGGAAGCCAAGAGGCCTCTTTATCAGTATCCACGACAAAGGGCATATTGCTTCAGTTCTTAACGCATGG
  CCAGAAGATGTTGTCAAGGCTATTGTGGTGACTGATGGAGAGCGAATCCTCGGCTTGGGCGACCTTGGTT
  GTAACGGGATGGGCATCCCTGTGGGTAAACTGGCCCTGTACACAGCGTGCGGAGGGGTGAATCCACAACA
  GTGTCTACCCATCACTTTGGACGTGGGCACAGAAAATGAGGAGTTACTTAAAGATCCCTTGTATATTGGG
  CTGCGGCACAGGCGAGTGAGAGGCCCTGAATATGATGCGTTTTTGGATGAATTCATGGAGGCAGCGTCTT
  CCAAATATGGCATGAATTGCCTTATTCAGTTTGAAGATTTTGCCAATCTGAATGCATTTCGTCTCCTGAA
  CAAGTATCGAAACAAGTATTGCACATTTAACGATGATATTCAAGGAACAGCGTCTGTGGCAGTTGCCGGC
  CTTCTTGCTGCTCTTCGGATAACCAAGAACAAGCTCTCTGATCAGACAGTGCTGTTCCAGGGAGCCGGCG
  AGGCTGCCTTGGGGATTGCTCATCTGATTGTTATGGCCATGGAGAAGGAAGGTTTATCAAAGGAGAAAGC
  TAGACAAAAGATATGGTTGGTTGACTCAAAAGGATTAATAGTTAAGGGGCGTGCTTCTCTCACAGAAGAG
  AAAGAGGTGTTTGCCCATGAACATGAAGAAATGAAGAACCTAGAAGCCATTGTTCAGAAGATAAAACCAA
  CCGCTCTCATAGGAGTTGCTGCAATTGGTGGTGCTTTCACAGAACAAATTCTCAAGGATATGGCTGCCTT
  CAACGAGCGGCCCATCATCTTTGCTTTGAGTAATCCGACCAGCAAAGCTGAGTGTTCTGCAGAGGAGTGC
  TATAAAGTGACCAAGGGCCGTGCGATCTTTGCCAGCGGCAGTCCTTTTGATCCAGTCACTCTTCCAGATG
  GACGGACTCTGTTTCCTGGCCAAGGCAACAACTCCTATGTGTTCCCTGGAGTTGCTCTTGGGGTAGTGGC
  CTGTGGACTGAGACACATCAATGATTCGGTCTTCCTCACCACGGCTGAGGTCATATCCCAGCAAGTGTCA
  GATAAACACCTAGAAGAAGGCCGGCTCTATCCTCCTTTGAACACCATCCGAGATGTTTCCTTGAAAATCG
  CAGTAAAGATTGTGCAAGATGCATACAAAGAAAAGATGGCCACTGTTTATCCTGAACCCCAAAACAAAGA
  AGAATTTGTCTCCTCCCAGATGTACAGCACTAATTATGACCAGATCCTACCTGATTGTTATTCGTGGCCT
  GAAGAAGTCCAGAAAATACAGACCAAAGTCAATCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203845 representing NM_012600
  Red=Cloning site Green=Tags(s)
  
  MDPRAPRRRHAHQRGYLLTRDPHLNKDLAFTLEERQQLNIHGLLPPCIVNQEIQVLRVIKNFERLNSDFD
  RYLLLMDLQDRNEKLFYSVLMSNVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAW
  PEDVVKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPLYIG
  LRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANLNAFRLLNKYRNKYCTFNDDIQGTASVAVAG
  LLAALRITKNKLSDQTVLFQGAGEAALGIAHLIVMAMEKEGLSKEKARQKIWLVDSKGLIVKGRASLTEE
  KEVFAHEHEEMKNLEAIVQKIKPTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSNPTSKAECSAEEC
  YKVTKGRAIFASGSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHINDSVFLTTAEVISQQVS
  DKHLEEGRLYPPLNTIRDVSLKIAVKIVQDAYKEKMATVYPEPQNKEEFVSSQMYSTNYDQILPDCYSWP
  EEVQKIQTKVNQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012600
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012600.2, NP_036732.2
• RefSeq Size: 3075
• RefSeq ORF: 1719
• Locus ID: 24552
• MW: 64 kDa
• Gene Summary: catalyzes the NADP+ dependent conversion of S-malate to pyruvate [RGD, Feb 2006]