Lss (NM_031049) Rat Tagged ORF Clone

CAT#: RR203867

Lss (Myc-DDK-tagged ORF) - Rat lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (Lss), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lss
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203867 representing NM_031049
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGGGCACGTGTCTGCGGCGTCGTGGGGGACCCTATAAAACTGAGCCCGCCACCGATCTCACCC
  GCTGGCGGCTCCATAATGAGTTGGGTCGGCAGAGATGGACTTATTATCAAGCGGAGGAGGACCCTGGTCG
  AGAACAGACGGGGCTAGAGGCCCACTCTTTGGGACTGGACACAACAAGTTATTTCAAGAACTTACCTAAA
  GCTCAAACAGCCCATGAGGGGGCCCTGAACGGAGTAACCTTTTATGCCAAGCTGCAGGCTGAGGATGGAC
  ACTGGGCTGGTGATTATGGTGGTCCACTCTTCCTCTTGCCAGGTCTCCTGATTACGTGTCACATAGCACA
  CATCCCCCTGCCGGCTGGATACAGAGAGGAAATGGTACGGTACTTGCGCTCAGTGCAGCTTCCCAATGGC
  GGCTGGGGCTTGCACATTGAGGACAAGTCCACGGTGTTTGGGACTGCCCTGAGCTATGTGTCTCTCAGAA
  TCCTGGGTATTGGACCTGATGATCCTGACCTTGTGCGTGCTCGGAACATTCTTCACAAAAAAGGTGGTGC
  AGTGGCCATCCCTTCCTGGGGGAAGTTCTGGCTGGCTGTCCTGAATGTTTACAGCTGGGAAGGAATCAAT
  ACCCTCTTCCCTGAGATGTGGCTGCTTCCTGAATGGTTTCCTGCACATCCCTCCACTCTGTGGTGTCACT
  GCCGGCAGGTCTATCTGCCCATGAGCTACTGCTACGCCACTCGGCTAAGTGCCTCAGAGGACCCACTGGT
  TCAGAGCCTCCGCCAGGAACTCTATGTGGAGGATTATGCCAGCATCGATTGGCCAGCACAGAAGAACAAC
  GTGTGCCCCGATGACATGTACACGCCACACAGCTGGCTGCTGCACGTGGTATATGGACTCCTCAACCTGT
  ATGAACGTTTCCACAGTACCAGCCTGCGGAAGTGGGCCATCCAGTTGCTGTATGAACATGTCGCAGCTGA
  TGATCGGTTCACGAAATGCATCAGCATTGGCCCGATCTCAAAAACTGTCAACATGCTTATTCGTTGGTCA
  GTGGACGGACCATCCTCCCCTGCCTTCCAGGAGCACGTCTCGAGGATCAAAGATTATCTTTGGCTGGGCC
  TTGACGGCATGAAAATGCAGGGCACCAATGGATCACAGACCTGGGACACTTCATTTGCTGTCCAAGCCCT
  GCTGGAGGCAGGTGCACACCGAAGACCTGAGTTTTTGCCCTGCCTGCAGAAGGCTCACGAGTTCCTGCGG
  CTTTCCCAGGTCCCAGACAACAATCCTGACTACCAGAAGTATTATCGCCATATGCACAAGGGTGGTTTCC
  CCTTCAGCACACTGGACTGTGGCTGGATCGTTGCTGACTGCACGGCCGAGGCTTTGAAGGCTGTGCTGCT
  CCTGCAGGAGCGGTGTCCCTCAATCACCGAGCATGTCCCCCGAGAGCGACTCTACGATGCTGTGGCTGTG
  TTGTTGAGCATGAGGAATTCCGATGGAGGGTTTGCTACCTATGAGACTAAGCGTGGCGGGTATTTGCTGG
  AGCTGCTGAACCCCTCAGAGGTCTTTGGAGACATCATGATTGACTACACGTATGTAGAGTGTACCTCCGC
  AGTGATGCAGGCCCTGAGGCACTTCCGCGAGTACTTCCCAGACCACAGGGCTACAGAGATCAGGGAGACC
  CTCAATCAAGGCCTGGACTTCTGCCGAAAGAAGCAGAGAGCGGATGGCTCGTGGGAGGGCTCCTGGGGGG
  TTTGCTTCACCTATGGCACCTGGTTTGGCTTGGAAGCATTCGCTTGCATGGGACATATCTACCAAAATAG
  GACTGCTTGTGCAGAAGTAGCTCAGGCCTGCCACTTCCTCCTGTCGCGGCAGATGGCGGATGGGGGCTGG
  GGGGAGGACTTTGAGTCCTGTGAGCAGCGGCGGTACGTGCAGAGTGCCGGGTCCCAGGTCCATAGTACGT
  GCTGGGCCCTGCTGGGTTTGATGGCTGTCAGGCATCCCGACATCTCTGCTCAGGAGAGAGGAATCAGATG
  TCTGCTAGGGAAACAGCTCCCCAACGGAGACTGGCCTCAGGAGAACATCTCTGGGGTCTTCAACAAGTCC
  TGTGCCATCAGCTACACAAATTACAGAAACATCTTCCCCATCTGGGCCCTGGGCCGCTTCTCCAGCCTGT
  ATCCTGACAATACCCTTGCTGGCCACATTATCCTGACAATACCCTTGCTGGCCACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203867 representing NM_031049
  Red=Cloning site Green=Tags(s)
  
  MTEGTCLRRRGGPYKTEPATDLTRWRLHNELGRQRWTYYQAEEDPGREQTGLEAHSLGLDTTSYFKNLPK
  AQTAHEGALNGVTFYAKLQAEDGHWAGDYGGPLFLLPGLLITCHIAHIPLPAGYREEMVRYLRSVQLPNG
  GWGLHIEDKSTVFGTALSYVSLRILGIGPDDPDLVRARNILHKKGGAVAIPSWGKFWLAVLNVYSWEGIN
  TLFPEMWLLPEWFPAHPSTLWCHCRQVYLPMSYCYATRLSASEDPLVQSLRQELYVEDYASIDWPAQKNN
  VCPDDMYTPHSWLLHVVYGLLNLYERFHSTSLRKWAIQLLYEHVAADDRFTKCISIGPISKTVNMLIRWS
  VDGPSSPAFQEHVSRIKDYLWLGLDGMKMQGTNGSQTWDTSFAVQALLEAGAHRRPEFLPCLQKAHEFLR
  LSQVPDNNPDYQKYYRHMHKGGFPFSTLDCGWIVADCTAEALKAVLLLQERCPSITEHVPRERLYDAVAV
  LLSMRNSDGGFATYETKRGGYLLELLNPSEVFGDIMIDYTYVECTSAVMQALRHFREYFPDHRATEIRET
  LNQGLDFCRKKQRADGSWEGSWGVCFTYGTWFGLEAFACMGHIYQNRTACAEVAQACHFLLSRQMADGGW
  GEDFESCEQRRYVQSAGSQVHSTCWALLGLMAVRHPDISAQERGIRCLLGKQLPNGDWPQENISGVFNKS
  CAISYTNYRNIFPIWALGRFSSLYPDNTLAGHIILTIPLLAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031049
• ORF Size: 2199 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq Size: 2851
• RefSeq ORF: 2202
• Locus ID: 81681
• MW: 83.3 kDa
• Gene Summary: enzyme that catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol [RGD, Feb 2006]