Shmt1 (NM_001047842) Rat Tagged ORF Clone

CAT#: RR203948

Shmt1 (Myc-DDK-tagged ORF) - Rat serine hydroxymethyltransferase 1 (soluble) (Shmt1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Shmt1
• Synonyms: LRRGT00032; mShmt; Shmt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203948 representing NM_001047842
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGACTGTGGAGGCATGGGTGCAGATATTCCTGCTTCCAGTTGAGAAGTCCGATCCTGTGTCGA
  GGCTGGACCCTGACAGGATGGGACATGTGAAAGGGCCAGTTCAGATGATCCAGAACTGCAGAATCTCCAC
  GACACAGGGCAACAACAGGATAACCCAGAGGAGTCCTGGATACACTCCAGATAGTCCTACAAAGTCCTTC
  TCACTGTGGAACAGCCAAAATCTGCTAGTTCAGTCCATGAAACTGGCTGCCTCAGCAGTCTCAATCTGGT
  TCTGGGGTTCTTGGAGGATTCCTAGAGAGCTGCTGGTTTTCAGTTTACATTGGAATCTTAAGGAAGTTGG
  ACTTAGCACCAGCCCCAGCAACAGCACAAACTTGCCAGCAAGAGTAAAAGGCAAGCAGGCAGAAAAGCAA
  AGCTTCCTTCTTCTAAGTTCTTTTATCTGGGTTGCCATCAGAAGCTACAGCTCCTTTTGTCAGGTTTTGC
  CCAAGTTTCTGCTCCGAGCTCGGGCGAATGGCGCCTGGGCGGCTGAGGGCGGGCTCTACCTGGACCCGCC
  CACTGATTGGGCCGCTCTAGGGGTGGGGCGGGGCCAGCCGCGCTGTGCAATGGCAGAGAGGGATGCCACC
  GTGTGGGCCTCTCATGAGAAGATGTTGACACAGCCCCTGAAGGAGAGCGATGCTGAGGTTTACAGCATCA
  TTAAAAAGGAGAGCAACCGGCAGAGGGTCGGATTGGAGCTGATTGCCTCGGAGAACTTTGCCAGCAGAGC
  TGTGTTGGAGGCCCTAGGATCTTGCTTAAATAACAAATACTCTGAGGGGTACCCAGGCCAAAGGTATTAT
  GGTGGGACTGAGTTCATCGATGAGCTGGAGACGCTGTGTCAGAAGCGTGCATTACAGGCCTACCATCTGG
  ATCCTCAGTGCTGGGGCGTCAATGTCCAGCCTTATTCAGGCTCCCCTGCAAACTTTGCTGTGTATACTGC
  CCTAGTAGAGCCCCATGGGCGAATCATGGGTTTGGACCTGCCAGATGGTGGCCATCTGACTCATGGCTTC
  ATGACAGACAAGAAGAAAATCTCTGCCACCTCTATCTTCTTTGAATCCATGCCTTATAAGGTATACCCAG
  ACACTGGGTACATCAACTATGACCAGCTAGAGGAGAATGCCAGCCTCTTCCACCCGAAGCTGATCATTGC
  AGGGACCAGCTGCTACTCCCGGAACCTGGACTATGCGCGGCTGCGAAAGATTGCGGATGATAATGGGGCA
  TATCTCATGGCAGACATGGCACACATCAGTGGGCTCGTGGCAGCTGGTGTGGTGCCCTCACCCTTTGAAC
  ACTGTCATGTAGTAACCACTACAACCCACAAGACCCTGAGAGGCTGCCGTGCTGGTATGATATTCTACAG
  AAAGGGTGTGCGCAGTGTTGATCCCAAGACTGGCGAAGAGACTTACTATGAACTGGAGTCCCTCATCAAC
  TCGGCCGTGTTCCCAGGCCTGCAGGGCGGCCCTCACAACCATGCCATTGCTGGTGTTGCTGTGGCCCTGA
  AGCAAGCCATGACTACAGAATTCAAAATCTACCAGCTCCAGGTGTTGGCCAACTGCAGGGCTCTGTCTGA
  TGCACTCACAGAGCTAGGCTACAAAATAGTCACAGGTGGCTCTGATAATCACTTGATCCTAATGGATCTG
  CGTCCTAAGGGTACTGATGGCGGAAGGGCTGAGAAGGTACTGGAAGCCTGTTCTATCGCCTGCAACAAGA
  ATACCTGCCCAGGTGACAAGAGTGCGTTACGGCCCAGTGGCCTGCGACTGGGAACTCCAGCATTGACATC
  CCGAGGACTTTTGGAAGAAGACTTCCAAAAAATAGCACACTTTATCCACAGAGGGATAGAGTTGACTCTG
  CAGATTCAGAGCCACATGACCATGAGGGCCACACTGAAAGAGTTCAAGGAGAAGCTGACAGGGGATGAGA
  AGTTCCAGAGTGCTGTGGCAGCTCTCCGGGAAGAAGTGGAGAATTTTGCTTCCAACTTCTCACTACCTGG
  ACTTCCTGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203948 representing NM_001047842
  Red=Cloning site Green=Tags(s)
  
  MELTVEAWVQIFLLPVEKSDPVSRLDPDRMGHVKGPVQMIQNCRISTTQGNNRITQRSPGYTPDSPTKSF
  SLWNSQNLLVQSMKLAASAVSIWFWGSWRIPRELLVFSLHWNLKEVGLSTSPSNSTNLPARVKGKQAEKQ
  SFLLLSSFIWVAIRSYSSFCQVLPKFLLRARANGAWAAEGGLYLDPPTDWAALGVGRGQPRCAMAERDAT
  VWASHEKMLTQPLKESDAEVYSIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYY
  GGTEFIDELETLCQKRALQAYHLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGF
  MTDKKKISATSIFFESMPYKVYPDTGYINYDQLEENASLFHPKLIIAGTSCYSRNLDYARLRKIADDNGA
  YLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVRSVDPKTGEETYYELESLIN
  SAVFPGLQGGPHNHAIAGVAVALKQAMTTEFKIYQLQVLANCRALSDALTELGYKIVTGGSDNHLILMDL
  RPKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSGLRLGTPALTSRGLLEEDFQKIAHFIHRGIELTL
  QIQSHMTMRATLKEFKEKLTGDEKFQSAVAALREEVENFASNFSLPGLPDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001047842
• ORF Size: 2043 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001047842.1, NP_001041307.1
• RefSeq Size: 2046
• RefSeq ORF: 2046
• Locus ID: 287379
• MW: 75.4 kDa