Mnda (NM_001012029) Rat Tagged ORF Clone

CAT#: RR203952

Ifi204 (Myc-DDK-tagged ORF) - Rat interferon activated gene 204 (Ifi204), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mnda
• Synonyms: Ifi204; rHin-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203952 representing NM_001012029
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAATGAATATAAGAGAATCGTTCTGTTGAAAGGACTTGAACCTCTCAGTAGTCATCATTTTAGCT
  TATTTAAGTCATTGCTGGCCAGTGATTTAAAACTGGAGAGACACATGCAGGAGCAATACACCAAGGTCCA
  GATTGCTGACATGATGGAAGATGAATTCCCAGATGATGCTGGATTGGGCAAATTCATCAAATTTTGTGAA
  GACTTACCAGCTCTTAGAAAACGTGCTGAAATTCTTAAAAGAGAGAGAATACAAGTAAGAGGAGAAACCC
  CACGGGAAATAAATAGGCGAGAACCAGGTCCGTCAAGACCTTCATCAACTGCAAGCCATATGATAGTGTC
  TGAAGGATGGGAGACTTCCACAGCTCAGGCAGAGACCTCCACAGCTCAGGGAGAGCCTTCTACAGCTCAG
  AAAAGAAAAAGTATGAGCGAAGGAAAGACTGAAGTGAAAAAGACCAAGGCATCAGAGAGACCAGATCAGC
  CTCCCTGTCCTGAAGAAGCCACAGCCAAGTGTCTGTCACCAATACCCCAGACTTCGTCTTTGGCTCCATG
  TAACTCTCCTTTGGCTAAGTGTTCAATGGAGAACCAAAATATACAGACCCAAAACCAGAACATTCCCAGA
  GGCGCTGTTCTCCAAACAGAACCCCTGACAGTGATGGTGCTCAAAGCAACAGACCCATTTGAATATGAAT
  CAGCAGAACATGGAGTGAAGAACATGTTTCTTGCTACAGTGGTTACTGTAGACCGGTATTTCCATGTGAA
  AGTTTTCAACAGCAACTTGAAAGAGAAGTTCAAAAAAAATAATTTTATCATCATTTCCAATTACTTCAAG
  AGGACAGACATCCTAGAGATCAATGAAGCTTCCCTTGTGTCAGAGGCTGCTCCTGACAAAAAGATTGCAG
  TGCCCAACAAACGTATCAAAGAGGCAAAAAAATCTCCTAAAATCCGTGATATTAAAGAGGGTACTTCAAG
  GGATCTGTTTTATGGAGTGTTTACATTAATCAAGAAAAAAGTGTGCCCAAAGAACACAATCTATGAACTA
  AAAGATGATACAGGAAATATAGAAGTGGTGGGGAGTGGAAAATGGTACAACATCAACTGTAACGAAGGAG
  ATAACTTCCAACTCTTCTGCTTTCACCTGAAAACAATTGACAGGCAACAAAAATTAGTGTGTGGAAAGCA
  CAGTTTTATCAAGGTCACCAGAGCTCGGGAAAAAAAGGAAGCAGCCACTGCCCATCCAAGCACAAAAAAG
  GAAGAAGGAACTCACTACCCTAAAGACAAATTCAATGCTTTTAAAGAAGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203952 representing NM_001012029
  Red=Cloning site Green=Tags(s)
  
  MSNEYKRIVLLKGLEPLSSHHFSLFKSLLASDLKLERHMQEQYTKVQIADMMEDEFPDDAGLGKFIKFCE
  DLPALRKRAEILKRERIQVRGETPREINRREPGPSRPSSTASHMIVSEGWETSTAQAETSTAQGEPSTAQ
  KRKSMSEGKTEVKKTKASERPDQPPCPEEATAKCLSPIPQTSSLAPCNSPLAKCSMENQNIQTQNQNIPR
  GAVLQTEPLTVMVLKATDPFEYESAEHGVKNMFLATVVTVDRYFHVKVFNSNLKEKFKKNNFIIISNYFK
  RTDILEINEASLVSEAAPDKKIAVPNKRIKEAKKSPKIRDIKEGTSRDLFYGVFTLIKKKVCPKNTIYEL
  KDDTGNIEVVGSGKWYNINCNEGDNFQLFCFHLKTIDRQQKLVCGKHSFIKVTRAREKKEAATAHPSTKK
  EEGTHYPKDKFNAFKEEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012029
• ORF Size: 1314 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012029.1, NP_001012029.1
• RefSeq Size: 1768 bp
• RefSeq ORF: 1317 bp
• Locus ID: 304988
• Cytogenetics: 13q24
• MW: 49.7 kDa